In vitro Selection of an RNA Epitope Immunologically Cross-Reactive with a Peptide
An antiserum raised against a peptide was used to select a unique RNA species from a degenerate pool of RNAs designed to resemble an autoantibody recognition site in U1 RNA. The peptide and the selected RNA epitope could compete for antibody binding, suggesting that both RNA and peptide epitopes occ...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1992-10, Vol.89 (19), p.8864-8868 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 89 |
| creator | Tsai, Donald E. Kenan, Daniel J. Keene, Jack D. |
| description | An antiserum raised against a peptide was used to select a unique RNA species from a degenerate pool of RNAs designed to resemble an autoantibody recognition site in U1 RNA. The peptide and the selected RNA epitope could compete for antibody binding, suggesting that both RNA and peptide epitopes occupy the same or overlapping antigen-combining sites. Thus, the RNA epitope functioned as a specific inhibitor of the antibody-antigen interaction. We demonstrate that the RNA epitope can be used to tag unrelated RNA molecules and also to detect the presence of the antibody. We propose that sequence-specific recognition of RNA by antibodies may involve protein-RNA contacts similar to those occurring in other nucleic acid-binding proteins. In addition, these findings are compatible with the suggestion that nucleic acid-binding autoantibodies may arise through immunological cross-reactivity between proteins and nucleic acids. |
| doi_str_mv | 10.1073/pnas.89.19.8864 |
| format | Article |
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The peptide and the selected RNA epitope could compete for antibody binding, suggesting that both RNA and peptide epitopes occupy the same or overlapping antigen-combining sites. Thus, the RNA epitope functioned as a specific inhibitor of the antibody-antigen interaction. We demonstrate that the RNA epitope can be used to tag unrelated RNA molecules and also to detect the presence of the antibody. We propose that sequence-specific recognition of RNA by antibodies may involve protein-RNA contacts similar to those occurring in other nucleic acid-binding proteins. In addition, these findings are compatible with the suggestion that nucleic acid-binding autoantibodies may arise through immunological cross-reactivity between proteins and nucleic acids.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.89.19.8864</identifier><identifier>PMID: 1384035</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Amino Acid Sequence ; Antibodies ; Antigenic determinants, haptens, artificial antigens ; Antigens ; Antiserum ; Autoantibodies ; Autoantibodies - immunology ; Base Sequence ; Biological and medical sciences ; Cellular biology ; Epitopes ; Epitopes - analysis ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; HeLa Cells ; Humans ; Immune Sera ; Immunity (Disease) ; Immunoprecipitation ; Molecular immunology ; Molecular Sequence Data ; Molecules ; Nucleic Acid Conformation ; Nucleotide sequences ; Nucleotides ; Oligodeoxyribonucleotides ; Oligoribonucleotides ; Peptides - chemical synthesis ; Peptides - immunology ; Promoter Regions, Genetic ; Restriction Mapping ; Ribonucleic acid ; RNA ; RNA - genetics ; RNA - immunology ; RNA, Small Nuclear - analysis ; RNA, Small Nuclear - genetics ; RNA, Small Nuclear - immunology ; Transcription, Genetic</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1992-10, Vol.89 (19), p.8864-8868</ispartof><rights>Copyright 1992 The National Academy of Sciences of the United States of America</rights><rights>1993 INIST-CNRS</rights><rights>Copyright National Academy of Sciences Oct 1, 1992</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4644-8562974e5d9bf5969ac6a446039b21814bae93923ca6dd01302b4a988dcaf9ab3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/89/19.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2360291$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2360291$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4398088$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1384035$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tsai, Donald E.</creatorcontrib><creatorcontrib>Kenan, Daniel J.</creatorcontrib><creatorcontrib>Keene, Jack D.</creatorcontrib><title>In vitro Selection of an RNA Epitope Immunologically Cross-Reactive with a Peptide</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>An antiserum raised against a peptide was used to select a unique RNA species from a degenerate pool of RNAs designed to resemble an autoantibody recognition site in U1 RNA. The peptide and the selected RNA epitope could compete for antibody binding, suggesting that both RNA and peptide epitopes occupy the same or overlapping antigen-combining sites. Thus, the RNA epitope functioned as a specific inhibitor of the antibody-antigen interaction. We demonstrate that the RNA epitope can be used to tag unrelated RNA molecules and also to detect the presence of the antibody. We propose that sequence-specific recognition of RNA by antibodies may involve protein-RNA contacts similar to those occurring in other nucleic acid-binding proteins. In addition, these findings are compatible with the suggestion that nucleic acid-binding autoantibodies may arise through immunological cross-reactivity between proteins and nucleic acids.</description><subject>Amino Acid Sequence</subject><subject>Antibodies</subject><subject>Antigenic determinants, haptens, artificial antigens</subject><subject>Antigens</subject><subject>Antiserum</subject><subject>Autoantibodies</subject><subject>Autoantibodies - immunology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cellular biology</subject><subject>Epitopes</subject><subject>Epitopes - analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Immune Sera</subject><subject>Immunity (Disease)</subject><subject>Immunoprecipitation</subject><subject>Molecular immunology</subject><subject>Molecular Sequence Data</subject><subject>Molecules</subject><subject>Nucleic Acid Conformation</subject><subject>Nucleotide sequences</subject><subject>Nucleotides</subject><subject>Oligodeoxyribonucleotides</subject><subject>Oligoribonucleotides</subject><subject>Peptides - chemical synthesis</subject><subject>Peptides - immunology</subject><subject>Promoter Regions, Genetic</subject><subject>Restriction Mapping</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA - genetics</subject><subject>RNA - immunology</subject><subject>RNA, Small Nuclear - analysis</subject><subject>RNA, Small Nuclear - genetics</subject><subject>RNA, Small Nuclear - immunology</subject><subject>Transcription, Genetic</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9v0zAcxS3ENErhzAWQhRCc0vlXXFviMlWDVZoAFThbTuJsrhI7s53C_nsctRTGAU4-vM_7fr9-D4BnGC0wWtKzwem4EHKB5UIIzh6AGUYSF5xJ9BDMECLLQjDCHoHHMW4RQrIU6BScYioYouUMbNYO7mwKHn4xnamT9Q76FmoHNx_P4cVgkx8MXPf96Hznr22tu-4OroKPsdgYnQ07A7_bdAM1_GyGZBvzBJy0uovm6eGdg2_vL76uLourTx_Wq_OromacsUKUnMglM2Ujq7aUXOqaa8Y4orIiWGBWaSOpJLTWvGkQpohUTEshmlq3Uld0Dt7t5w5j1ZumNi4F3akh2F6HO-W1VfcVZ2_Utd-pMsfCsv3NwR787WhiUr2Ntek67Ywfo1pSUlJB8X9BzCkqJSsz-OovcOvH4HIGiuT7l5TleXNwtofqKcNg2uPBGKmpUjVVqoRUWKqp0ux48ec_f_P7DrP--qDrmOtpg3a1jUeMUSmQmBa_PGDT_F_qvT1v_wmoduy6ZH6kTD7fk9uYfDiihHJEJKY_AbUzyoc</recordid><startdate>19921001</startdate><enddate>19921001</enddate><creator>Tsai, Donald E.</creator><creator>Kenan, Daniel J.</creator><creator>Keene, Jack D.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><general>National Academy of Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19921001</creationdate><title>In vitro Selection of an RNA Epitope Immunologically Cross-Reactive with a Peptide</title><author>Tsai, Donald E. ; Kenan, Daniel J. ; Keene, Jack D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4644-8562974e5d9bf5969ac6a446039b21814bae93923ca6dd01302b4a988dcaf9ab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Amino Acid Sequence</topic><topic>Antibodies</topic><topic>Antigenic determinants, haptens, artificial antigens</topic><topic>Antigens</topic><topic>Antiserum</topic><topic>Autoantibodies</topic><topic>Autoantibodies - immunology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cellular biology</topic><topic>Epitopes</topic><topic>Epitopes - analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Immune Sera</topic><topic>Immunity (Disease)</topic><topic>Immunoprecipitation</topic><topic>Molecular immunology</topic><topic>Molecular Sequence Data</topic><topic>Molecules</topic><topic>Nucleic Acid Conformation</topic><topic>Nucleotide sequences</topic><topic>Nucleotides</topic><topic>Oligodeoxyribonucleotides</topic><topic>Oligoribonucleotides</topic><topic>Peptides - chemical synthesis</topic><topic>Peptides - immunology</topic><topic>Promoter Regions, Genetic</topic><topic>Restriction Mapping</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA - genetics</topic><topic>RNA - immunology</topic><topic>RNA, Small Nuclear - analysis</topic><topic>RNA, Small Nuclear - genetics</topic><topic>RNA, Small Nuclear - immunology</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tsai, Donald E.</creatorcontrib><creatorcontrib>Kenan, Daniel J.</creatorcontrib><creatorcontrib>Keene, Jack D.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tsai, Donald E.</au><au>Kenan, Daniel J.</au><au>Keene, Jack D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro Selection of an RNA Epitope Immunologically Cross-Reactive with a Peptide</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1992-10-01</date><risdate>1992</risdate><volume>89</volume><issue>19</issue><spage>8864</spage><epage>8868</epage><pages>8864-8868</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>An antiserum raised against a peptide was used to select a unique RNA species from a degenerate pool of RNAs designed to resemble an autoantibody recognition site in U1 RNA. The peptide and the selected RNA epitope could compete for antibody binding, suggesting that both RNA and peptide epitopes occupy the same or overlapping antigen-combining sites. Thus, the RNA epitope functioned as a specific inhibitor of the antibody-antigen interaction. We demonstrate that the RNA epitope can be used to tag unrelated RNA molecules and also to detect the presence of the antibody. We propose that sequence-specific recognition of RNA by antibodies may involve protein-RNA contacts similar to those occurring in other nucleic acid-binding proteins. In addition, these findings are compatible with the suggestion that nucleic acid-binding autoantibodies may arise through immunological cross-reactivity between proteins and nucleic acids.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>1384035</pmid><doi>10.1073/pnas.89.19.8864</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1992-10, Vol.89 (19), p.8864-8868 |
| issn | 0027-8424 1091-6490 |
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| recordid | cdi_pnas_primary_89_19_8864 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Amino Acid Sequence Antibodies Antigenic determinants, haptens, artificial antigens Antigens Antiserum Autoantibodies Autoantibodies - immunology Base Sequence Biological and medical sciences Cellular biology Epitopes Epitopes - analysis Fundamental and applied biological sciences. Psychology Fundamental immunology HeLa Cells Humans Immune Sera Immunity (Disease) Immunoprecipitation Molecular immunology Molecular Sequence Data Molecules Nucleic Acid Conformation Nucleotide sequences Nucleotides Oligodeoxyribonucleotides Oligoribonucleotides Peptides - chemical synthesis Peptides - immunology Promoter Regions, Genetic Restriction Mapping Ribonucleic acid RNA RNA - genetics RNA - immunology RNA, Small Nuclear - analysis RNA, Small Nuclear - genetics RNA, Small Nuclear - immunology Transcription, Genetic |
| title | In vitro Selection of an RNA Epitope Immunologically Cross-Reactive with a Peptide |
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