Stable genetic transformation of a beneficial arthropod, Metaseiulus occidentalis (Acari: Phytoseiidae), by a microinjection technique
A microinjection technique has resulted in stable transformation of the western predatory mite Metaseiulus occidentalis. Early preblastoderm eggs within gravid females were microinjected. The needle was inserted through the cuticle of gravid females into the egg, or the tissue immediately surroundin...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1992-08, Vol.89 (16), p.7732-7736 |
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| creator | Presnail, James K. Hoy, Marjorie A. |
| description | A microinjection technique has resulted in stable transformation of the western predatory mite Metaseiulus occidentalis. Early preblastoderm eggs within gravid females were microinjected. The needle was inserted through the cuticle of gravid females into the egg, or the tissue immediately surrounding the egg. This maternal injection method resulted in relatively high levels of survival and transformation. Transformation was achieved without the aid of any transposase-producing helper plasmid. The predatory mite was transformed with a plasmid containing the Escherichia coli beta-galactosidase gene (lacZ) regulated by the Drosophila hsp70 heat-shock promoter. Putatively transformed lines were isolated based on beta-galactosidase activity in first-generation larvae. Transformation was confirmed in the sixth generation by polymerase chain reaction amplification of a region spanning the Drosophila/E. coli sequences. Amplification of a nested region, also spanning the interspecific boundary, provided further evidence for stable transformation. Maternal microinjection may be adaptable to other beneficial arthropods, particularly other phytoseiid mites. Genetic transformation of M. occidentalis may improve its efficiency as a biological control agent as well as provide a method for investigating details of its physiology and ecology. |
| doi_str_mv | 10.1073/pnas.89.16.7732 |
| format | Article |
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Early preblastoderm eggs within gravid females were microinjected. The needle was inserted through the cuticle of gravid females into the egg, or the tissue immediately surrounding the egg. This maternal injection method resulted in relatively high levels of survival and transformation. Transformation was achieved without the aid of any transposase-producing helper plasmid. The predatory mite was transformed with a plasmid containing the Escherichia coli beta-galactosidase gene (lacZ) regulated by the Drosophila hsp70 heat-shock promoter. Putatively transformed lines were isolated based on beta-galactosidase activity in first-generation larvae. Transformation was confirmed in the sixth generation by polymerase chain reaction amplification of a region spanning the Drosophila/E. coli sequences. Amplification of a nested region, also spanning the interspecific boundary, provided further evidence for stable transformation. Maternal microinjection may be adaptable to other beneficial arthropods, particularly other phytoseiid mites. Genetic transformation of M. occidentalis may improve its efficiency as a biological control agent as well as provide a method for investigating details of its physiology and ecology.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.89.16.7732</identifier><identifier>PMID: 1502192</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Acari ; Animals ; arthropode utile ; Arthropods ; Arthropods - genetics ; artropodos utiles ; auxiliaire de lutte biologique ; Base Sequence ; beta-Galactosidase - genetics ; beta-Galactosidase - metabolism ; Biological and medical sciences ; biological control organisms ; Biotechnology ; breeding methods ; control de insectos ; depredadores ; Drosophila ; Drosophila melanogaster - genetics ; Eggs ; Escherichia coli - enzymology ; Escherichia coli - genetics ; Female ; Female animals ; Fundamental and applied biological sciences. Psychology ; Genetic engineering ; Genetic technics ; Genetic Techniques ; genetic transformation ; Genetic vectors ; Heat-Shock Proteins - genetics ; Heat-Shock Proteins - metabolism ; insect control ; insecte nuisible ; insectos daninos ; Invertebrates ; Larva ; lutte antiinsecte ; Metaseiulus occidentalis ; methode d' amelioration ; Methods. Procedures. Technologies ; metodos de mejoramiento ; Microinjections ; Mites ; Molecular Sequence Data ; Oligodeoxyribonucleotides ; organismos para control biologico ; Ova ; pest insects ; pests of plants ; Phytoseiidae ; plagas de plantas ; Plasmids ; Polymerase chain reaction ; Polymerase Chain Reaction - methods ; predateur ; predators ; Promoter Regions, Genetic ; ravageur des plantes ; Recombinant Fusion Proteins - metabolism ; Restriction Mapping ; transformacion genetica ; transformation genetique ; Transformation, Genetic ; Transgenic animals ; Transgenic animals and transgenic plants ; useful arthropods</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1992-08, Vol.89 (16), p.7732-7736</ispartof><rights>Copyright 1992 The National Academy of Sciences of the United States of America</rights><rights>1992 INIST-CNRS</rights><rights>Copyright National Academy of Sciences Aug 15, 1992</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c638t-f58725c3dfa49bf8c6abbdb6f92c01d26375a66003ee96ebf833baf100d4c6463</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/89/16.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2360165$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2360165$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5546152$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1502192$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Presnail, James K.</creatorcontrib><creatorcontrib>Hoy, Marjorie A.</creatorcontrib><creatorcontrib>University of California, Berkeley, CA</creatorcontrib><title>Stable genetic transformation of a beneficial arthropod, Metaseiulus occidentalis (Acari: Phytoseiidae), by a microinjection technique</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>A microinjection technique has resulted in stable transformation of the western predatory mite Metaseiulus occidentalis. Early preblastoderm eggs within gravid females were microinjected. The needle was inserted through the cuticle of gravid females into the egg, or the tissue immediately surrounding the egg. This maternal injection method resulted in relatively high levels of survival and transformation. Transformation was achieved without the aid of any transposase-producing helper plasmid. The predatory mite was transformed with a plasmid containing the Escherichia coli beta-galactosidase gene (lacZ) regulated by the Drosophila hsp70 heat-shock promoter. Putatively transformed lines were isolated based on beta-galactosidase activity in first-generation larvae. Transformation was confirmed in the sixth generation by polymerase chain reaction amplification of a region spanning the Drosophila/E. coli sequences. Amplification of a nested region, also spanning the interspecific boundary, provided further evidence for stable transformation. Maternal microinjection may be adaptable to other beneficial arthropods, particularly other phytoseiid mites. Genetic transformation of M. occidentalis may improve its efficiency as a biological control agent as well as provide a method for investigating details of its physiology and ecology.</description><subject>Acari</subject><subject>Animals</subject><subject>arthropode utile</subject><subject>Arthropods</subject><subject>Arthropods - genetics</subject><subject>artropodos utiles</subject><subject>auxiliaire de lutte biologique</subject><subject>Base Sequence</subject><subject>beta-Galactosidase - genetics</subject><subject>beta-Galactosidase - metabolism</subject><subject>Biological and medical sciences</subject><subject>biological control organisms</subject><subject>Biotechnology</subject><subject>breeding methods</subject><subject>control de insectos</subject><subject>depredadores</subject><subject>Drosophila</subject><subject>Drosophila melanogaster - genetics</subject><subject>Eggs</subject><subject>Escherichia coli - enzymology</subject><subject>Escherichia coli - genetics</subject><subject>Female</subject><subject>Female animals</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Genetic Techniques</subject><subject>genetic transformation</subject><subject>Genetic vectors</subject><subject>Heat-Shock Proteins - genetics</subject><subject>Heat-Shock Proteins - metabolism</subject><subject>insect control</subject><subject>insecte nuisible</subject><subject>insectos daninos</subject><subject>Invertebrates</subject><subject>Larva</subject><subject>lutte antiinsecte</subject><subject>Metaseiulus occidentalis</subject><subject>methode d' amelioration</subject><subject>Methods. Procedures. Technologies</subject><subject>metodos de mejoramiento</subject><subject>Microinjections</subject><subject>Mites</subject><subject>Molecular Sequence Data</subject><subject>Oligodeoxyribonucleotides</subject><subject>organismos para control biologico</subject><subject>Ova</subject><subject>pest insects</subject><subject>pests of plants</subject><subject>Phytoseiidae</subject><subject>plagas de plantas</subject><subject>Plasmids</subject><subject>Polymerase chain reaction</subject><subject>Polymerase Chain Reaction - methods</subject><subject>predateur</subject><subject>predators</subject><subject>Promoter Regions, Genetic</subject><subject>ravageur des plantes</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>Restriction Mapping</subject><subject>transformacion genetica</subject><subject>transformation genetique</subject><subject>Transformation, Genetic</subject><subject>Transgenic animals</subject><subject>Transgenic animals and transgenic plants</subject><subject>useful arthropods</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkktvEzEUhUcIVEphzQaQhRAPqUn9njFiU1W8pCKQStfWHY-dOJqMg-1B5A_wu3FISIAFrLw43zk-9r1VdZ_gKcE1O1sNkKaNmhI5rWtGb1THBCsykVzhm9UxxrSeNJzy29WdlBYYYyUafFQdEYEpUfS4-n6Voe0tmtnBZm9QjjAkF-ISsg8DCg4BaovmvPHQI4h5HsMqdKfog82QrB_7MaFgjO_skKH3CT0_NxD9S_Rpvs6hEL4D--IUtesStfQmBj8srPkZn62ZD_7LaO9Wtxz0yd7bnSfV9ZvXny_eTS4_vn1_cX45MZI1eeJEU1NhWOeAq9Y1RkLbdq10ihpMOipZLUBKjJm1StpCMNaCIxh33Egu2Un1apu7Gtul7UzpHKHXq-iXENc6gNd_KoOf61n4qrmqG1HsT3f2GErrlPXSJ2P7HgYbxqRrRqjgWP0XJJLWXFFWwMd_gYswxqH8gaaYUNlISQt0toXK56UUrdsXJlhv1kBv1kA3quTqzRoUx8Pf33ngt3Mv-pOdDslA78rUjU97TAguidhgz3bYJv-XerhHu7Hvs_2WC_non2QBHmyBRcoh7gnKJCZSHPwOgoZZLGWur4hSrMxSMk7ZD02V6J8</recordid><startdate>19920815</startdate><enddate>19920815</enddate><creator>Presnail, James K.</creator><creator>Hoy, Marjorie A.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><general>National Academy of Sciences</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7QO</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19920815</creationdate><title>Stable genetic transformation of a beneficial arthropod, Metaseiulus occidentalis (Acari: Phytoseiidae), by a microinjection technique</title><author>Presnail, James K. ; Hoy, Marjorie A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c638t-f58725c3dfa49bf8c6abbdb6f92c01d26375a66003ee96ebf833baf100d4c6463</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Acari</topic><topic>Animals</topic><topic>arthropode utile</topic><topic>Arthropods</topic><topic>Arthropods - genetics</topic><topic>artropodos utiles</topic><topic>auxiliaire de lutte biologique</topic><topic>Base Sequence</topic><topic>beta-Galactosidase - genetics</topic><topic>beta-Galactosidase - metabolism</topic><topic>Biological and medical sciences</topic><topic>biological control organisms</topic><topic>Biotechnology</topic><topic>breeding methods</topic><topic>control de insectos</topic><topic>depredadores</topic><topic>Drosophila</topic><topic>Drosophila melanogaster - genetics</topic><topic>Eggs</topic><topic>Escherichia coli - enzymology</topic><topic>Escherichia coli - genetics</topic><topic>Female</topic><topic>Female animals</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>Genetic Techniques</topic><topic>genetic transformation</topic><topic>Genetic vectors</topic><topic>Heat-Shock Proteins - genetics</topic><topic>Heat-Shock Proteins - metabolism</topic><topic>insect control</topic><topic>insecte nuisible</topic><topic>insectos daninos</topic><topic>Invertebrates</topic><topic>Larva</topic><topic>lutte antiinsecte</topic><topic>Metaseiulus occidentalis</topic><topic>methode d' amelioration</topic><topic>Methods. Procedures. Technologies</topic><topic>metodos de mejoramiento</topic><topic>Microinjections</topic><topic>Mites</topic><topic>Molecular Sequence Data</topic><topic>Oligodeoxyribonucleotides</topic><topic>organismos para control biologico</topic><topic>Ova</topic><topic>pest insects</topic><topic>pests of plants</topic><topic>Phytoseiidae</topic><topic>plagas de plantas</topic><topic>Plasmids</topic><topic>Polymerase chain reaction</topic><topic>Polymerase Chain Reaction - methods</topic><topic>predateur</topic><topic>predators</topic><topic>Promoter Regions, Genetic</topic><topic>ravageur des plantes</topic><topic>Recombinant Fusion Proteins - metabolism</topic><topic>Restriction Mapping</topic><topic>transformacion genetica</topic><topic>transformation genetique</topic><topic>Transformation, Genetic</topic><topic>Transgenic animals</topic><topic>Transgenic animals and transgenic plants</topic><topic>useful arthropods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Presnail, James K.</creatorcontrib><creatorcontrib>Hoy, Marjorie A.</creatorcontrib><creatorcontrib>University of California, Berkeley, CA</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Presnail, James K.</au><au>Hoy, Marjorie A.</au><aucorp>University of California, Berkeley, CA</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Stable genetic transformation of a beneficial arthropod, Metaseiulus occidentalis (Acari: Phytoseiidae), by a microinjection technique</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1992-08-15</date><risdate>1992</risdate><volume>89</volume><issue>16</issue><spage>7732</spage><epage>7736</epage><pages>7732-7736</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>A microinjection technique has resulted in stable transformation of the western predatory mite Metaseiulus occidentalis. Early preblastoderm eggs within gravid females were microinjected. The needle was inserted through the cuticle of gravid females into the egg, or the tissue immediately surrounding the egg. This maternal injection method resulted in relatively high levels of survival and transformation. Transformation was achieved without the aid of any transposase-producing helper plasmid. The predatory mite was transformed with a plasmid containing the Escherichia coli beta-galactosidase gene (lacZ) regulated by the Drosophila hsp70 heat-shock promoter. Putatively transformed lines were isolated based on beta-galactosidase activity in first-generation larvae. Transformation was confirmed in the sixth generation by polymerase chain reaction amplification of a region spanning the Drosophila/E. coli sequences. Amplification of a nested region, also spanning the interspecific boundary, provided further evidence for stable transformation. Maternal microinjection may be adaptable to other beneficial arthropods, particularly other phytoseiid mites. Genetic transformation of M. occidentalis may improve its efficiency as a biological control agent as well as provide a method for investigating details of its physiology and ecology.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>1502192</pmid><doi>10.1073/pnas.89.16.7732</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1992-08, Vol.89 (16), p.7732-7736 |
| issn | 0027-8424 1091-6490 |
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| recordid | cdi_pnas_primary_89_16_7732 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Acari Animals arthropode utile Arthropods Arthropods - genetics artropodos utiles auxiliaire de lutte biologique Base Sequence beta-Galactosidase - genetics beta-Galactosidase - metabolism Biological and medical sciences biological control organisms Biotechnology breeding methods control de insectos depredadores Drosophila Drosophila melanogaster - genetics Eggs Escherichia coli - enzymology Escherichia coli - genetics Female Female animals Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Genetic Techniques genetic transformation Genetic vectors Heat-Shock Proteins - genetics Heat-Shock Proteins - metabolism insect control insecte nuisible insectos daninos Invertebrates Larva lutte antiinsecte Metaseiulus occidentalis methode d' amelioration Methods. Procedures. Technologies metodos de mejoramiento Microinjections Mites Molecular Sequence Data Oligodeoxyribonucleotides organismos para control biologico Ova pest insects pests of plants Phytoseiidae plagas de plantas Plasmids Polymerase chain reaction Polymerase Chain Reaction - methods predateur predators Promoter Regions, Genetic ravageur des plantes Recombinant Fusion Proteins - metabolism Restriction Mapping transformacion genetica transformation genetique Transformation, Genetic Transgenic animals Transgenic animals and transgenic plants useful arthropods |
| title | Stable genetic transformation of a beneficial arthropod, Metaseiulus occidentalis (Acari: Phytoseiidae), by a microinjection technique |
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