Antibodies to a peptide from the maize auxin-binding protein have auxin agonist activity
The major auxin-binding protein in maize membranes is thought to function as a physiological receptor. From earlier information, including the use of site-directed irreversible inhibitors, several of the amino acids likely to form part of the active auxin-binding site were provisionally assigned. In...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1992-08, Vol.89 (15), p.7208-7212 |
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| creator | Venis, M.A. (Horticulture Research International, East Malling, Kent, UK) Napier, R.M Barbier-Brygoo, H Maurel, C Perrot-Rechenmann, C Guern, J |
| description | The major auxin-binding protein in maize membranes is thought to function as a physiological receptor. From earlier information, including the use of site-directed irreversible inhibitors, several of the amino acids likely to form part of the active auxin-binding site were provisionally assigned. Inspection of the amino acid sequence of the auxin-binding protein showed a short region containing all but one of these amino acids. We find that antisera raised against a synthetic peptide encompassing this region recognize all isoforms of the maize auxin-binding protein together with homologous polypeptides in other species. We further find that the antibodies hyperpolarize protoplast transmembrane potential in an auxin-like manner. We conclude that these antibodies display auxin agonist activity and that we have identified an essential portion of the auxin-binding site. |
| doi_str_mv | 10.1073/pnas.89.15.7208 |
| format | Article |
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From earlier information, including the use of site-directed irreversible inhibitors, several of the amino acids likely to form part of the active auxin-binding site were provisionally assigned. Inspection of the amino acid sequence of the auxin-binding protein showed a short region containing all but one of these amino acids. We find that antisera raised against a synthetic peptide encompassing this region recognize all isoforms of the maize auxin-binding protein together with homologous polypeptides in other species. We further find that the antibodies hyperpolarize protoplast transmembrane potential in an auxin-like manner. We conclude that these antibodies display auxin agonist activity and that we have identified an essential portion of the auxin-binding site.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.89.15.7208</identifier><identifier>PMID: 1323130</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>activity ; Agonists ; Amino Acid Sequence ; Amino acids ; Analytical, structural and metabolic biochemistry ; Antibodies ; ANTICORPS ; ANTICUERPOS ; Antiserum ; auxin-binding protein ; AUXINAS ; AUXINE ; Auxins ; Biological and medical sciences ; Cell membranes ; Cellular Biology ; Corn ; Fundamental and applied biological sciences. Psychology ; Indoleacetic Acids - metabolism ; Intracellular Membranes - drug effects ; Intracellular Membranes - physiology ; Life Sciences ; LIGAND ; LIGANDOS ; MEMBRANA ; MEMBRANE ; membrane potential ; Membrane Proteins - isolation & purification ; Membranes ; Microsomes - drug effects ; Microsomes - physiology ; Molecular Sequence Data ; Molecular Weight ; Naphthaleneacetic Acids - pharmacology ; Other biological molecules ; PEPTIDE ; Peptides - chemical synthesis ; Peptides - immunology ; PEPTIDOS ; Plant Growth Regulators ; Plant growth substances ; Plant Physiological Phenomena ; Plant Proteins ; Protein isoforms ; PROTEINAS ; PROTEINE ; Proteins ; Protoplasts ; Protoplasts - physiology ; Receptors ; Receptors, Cell Surface - immunology ; Receptors, Cell Surface - isolation & purification ; Receptors, Cell Surface - metabolism ; Space life sciences ; ZEA MAYS ; Zea mays - physiology</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1992-08, Vol.89 (15), p.7208-7212</ispartof><rights>Copyright 1992 The National Academy of Sciences of the United States of America</rights><rights>1992 INIST-CNRS</rights><rights>Copyright National Academy of Sciences Aug 1, 1992</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c668t-6865b03b457655d9be0bc7b0afcdba2ef9bd65dfa7bfefadc02f4475f6431913</citedby><orcidid>0000-0002-4255-6440</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/89/15.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2359961$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2359961$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5499944$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1323130$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02707533$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Venis, M.A. (Horticulture Research International, East Malling, Kent, UK)</creatorcontrib><creatorcontrib>Napier, R.M</creatorcontrib><creatorcontrib>Barbier-Brygoo, H</creatorcontrib><creatorcontrib>Maurel, C</creatorcontrib><creatorcontrib>Perrot-Rechenmann, C</creatorcontrib><creatorcontrib>Guern, J</creatorcontrib><title>Antibodies to a peptide from the maize auxin-binding protein have auxin agonist activity</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The major auxin-binding protein in maize membranes is thought to function as a physiological receptor. From earlier information, including the use of site-directed irreversible inhibitors, several of the amino acids likely to form part of the active auxin-binding site were provisionally assigned. Inspection of the amino acid sequence of the auxin-binding protein showed a short region containing all but one of these amino acids. We find that antisera raised against a synthetic peptide encompassing this region recognize all isoforms of the maize auxin-binding protein together with homologous polypeptides in other species. We further find that the antibodies hyperpolarize protoplast transmembrane potential in an auxin-like manner. We conclude that these antibodies display auxin agonist activity and that we have identified an essential portion of the auxin-binding site.</description><subject>activity</subject><subject>Agonists</subject><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Antibodies</subject><subject>ANTICORPS</subject><subject>ANTICUERPOS</subject><subject>Antiserum</subject><subject>auxin-binding protein</subject><subject>AUXINAS</subject><subject>AUXINE</subject><subject>Auxins</subject><subject>Biological and medical sciences</subject><subject>Cell membranes</subject><subject>Cellular Biology</subject><subject>Corn</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Indoleacetic Acids - metabolism</subject><subject>Intracellular Membranes - drug effects</subject><subject>Intracellular Membranes - physiology</subject><subject>Life Sciences</subject><subject>LIGAND</subject><subject>LIGANDOS</subject><subject>MEMBRANA</subject><subject>MEMBRANE</subject><subject>membrane potential</subject><subject>Membrane Proteins - isolation & purification</subject><subject>Membranes</subject><subject>Microsomes - drug effects</subject><subject>Microsomes - physiology</subject><subject>Molecular Sequence Data</subject><subject>Molecular Weight</subject><subject>Naphthaleneacetic Acids - pharmacology</subject><subject>Other biological molecules</subject><subject>PEPTIDE</subject><subject>Peptides - chemical synthesis</subject><subject>Peptides - immunology</subject><subject>PEPTIDOS</subject><subject>Plant Growth Regulators</subject><subject>Plant growth substances</subject><subject>Plant Physiological Phenomena</subject><subject>Plant Proteins</subject><subject>Protein isoforms</subject><subject>PROTEINAS</subject><subject>PROTEINE</subject><subject>Proteins</subject><subject>Protoplasts</subject><subject>Protoplasts - physiology</subject><subject>Receptors</subject><subject>Receptors, Cell Surface - immunology</subject><subject>Receptors, Cell Surface - isolation & purification</subject><subject>Receptors, Cell Surface - metabolism</subject><subject>Space life sciences</subject><subject>ZEA MAYS</subject><subject>Zea mays - physiology</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks-L1DAUx4so67p6FkQliCgeOps0P9qAl2FRVxjw4AreQtImMxk6SU3SYde_3pSOo7sHPQXy_Xzzfe_lFcVTBBcI1vh8cDIuGr5AdFFXsLlXnCLIUckIh_eLUwirumxIRR4Wj2LcQgg5beBJcYJwhRGGp8X3pUtW-c7qCJIHEgx6SLbTwAS_A2mjwU7anxrI8dq6UlnXWbcGQ_BJWwc2cn-QgFx7Z2MCsk12b9PN4-KBkX3UTw7nWXH18cPVxWW5-vLp88VyVbaMNalkDaMKYkVozSjtuNJQtbWC0rSdkpU2XHWMdkbWymgjuxZWhpCaGkYw4gifFe_nZ4dR7XTXapeC7MUQ7E6GG-GlFbcVZzdi7feCcFbTbH832zd3TJfLlZju8gBh5vB-inpziAr-x6hjEjsbW9330mk_RlFjyDGG8L8gYlXdVAxn8NUdcOvH4PK4RAVR1XCOp9jzGWqDjzFoc6wTQTHtgJh2QDRcICqmHciOF3_P5A8_f3rWXx90GVvZmyBda-MRo4RzTkjGXh6w6f3f6q2ct_8EhBn7PunrlMnnM7mNyYcjWmHKOZtafDbLRnoh1yEX8-1r7n0Kwb8AYknkoA</recordid><startdate>19920801</startdate><enddate>19920801</enddate><creator>Venis, M.A. (Horticulture Research International, East Malling, Kent, UK)</creator><creator>Napier, R.M</creator><creator>Barbier-Brygoo, H</creator><creator>Maurel, C</creator><creator>Perrot-Rechenmann, C</creator><creator>Guern, J</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><general>National Academy of Sciences</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>M81</scope><scope>7X8</scope><scope>1XC</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-4255-6440</orcidid></search><sort><creationdate>19920801</creationdate><title>Antibodies to a peptide from the maize auxin-binding protein have auxin agonist activity</title><author>Venis, M.A. (Horticulture Research International, East Malling, Kent, UK) ; Napier, R.M ; Barbier-Brygoo, H ; Maurel, C ; Perrot-Rechenmann, C ; Guern, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c668t-6865b03b457655d9be0bc7b0afcdba2ef9bd65dfa7bfefadc02f4475f6431913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>activity</topic><topic>Agonists</topic><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Antibodies</topic><topic>ANTICORPS</topic><topic>ANTICUERPOS</topic><topic>Antiserum</topic><topic>auxin-binding protein</topic><topic>AUXINAS</topic><topic>AUXINE</topic><topic>Auxins</topic><topic>Biological and medical sciences</topic><topic>Cell membranes</topic><topic>Cellular Biology</topic><topic>Corn</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Indoleacetic Acids - metabolism</topic><topic>Intracellular Membranes - drug effects</topic><topic>Intracellular Membranes - physiology</topic><topic>Life Sciences</topic><topic>LIGAND</topic><topic>LIGANDOS</topic><topic>MEMBRANA</topic><topic>MEMBRANE</topic><topic>membrane potential</topic><topic>Membrane Proteins - isolation & purification</topic><topic>Membranes</topic><topic>Microsomes - drug effects</topic><topic>Microsomes - physiology</topic><topic>Molecular Sequence Data</topic><topic>Molecular Weight</topic><topic>Naphthaleneacetic Acids - pharmacology</topic><topic>Other biological molecules</topic><topic>PEPTIDE</topic><topic>Peptides - chemical synthesis</topic><topic>Peptides - immunology</topic><topic>PEPTIDOS</topic><topic>Plant Growth Regulators</topic><topic>Plant growth substances</topic><topic>Plant Physiological Phenomena</topic><topic>Plant Proteins</topic><topic>Protein isoforms</topic><topic>PROTEINAS</topic><topic>PROTEINE</topic><topic>Proteins</topic><topic>Protoplasts</topic><topic>Protoplasts - physiology</topic><topic>Receptors</topic><topic>Receptors, Cell Surface - immunology</topic><topic>Receptors, Cell Surface - isolation & purification</topic><topic>Receptors, Cell Surface - metabolism</topic><topic>Space life sciences</topic><topic>ZEA MAYS</topic><topic>Zea mays - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Venis, M.A. (Horticulture Research International, East Malling, Kent, UK)</creatorcontrib><creatorcontrib>Napier, R.M</creatorcontrib><creatorcontrib>Barbier-Brygoo, H</creatorcontrib><creatorcontrib>Maurel, C</creatorcontrib><creatorcontrib>Perrot-Rechenmann, C</creatorcontrib><creatorcontrib>Guern, J</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Biochemistry Abstracts 3</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Venis, M.A. (Horticulture Research International, East Malling, Kent, UK)</au><au>Napier, R.M</au><au>Barbier-Brygoo, H</au><au>Maurel, C</au><au>Perrot-Rechenmann, C</au><au>Guern, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antibodies to a peptide from the maize auxin-binding protein have auxin agonist activity</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1992-08-01</date><risdate>1992</risdate><volume>89</volume><issue>15</issue><spage>7208</spage><epage>7212</epage><pages>7208-7212</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>The major auxin-binding protein in maize membranes is thought to function as a physiological receptor. From earlier information, including the use of site-directed irreversible inhibitors, several of the amino acids likely to form part of the active auxin-binding site were provisionally assigned. Inspection of the amino acid sequence of the auxin-binding protein showed a short region containing all but one of these amino acids. We find that antisera raised against a synthetic peptide encompassing this region recognize all isoforms of the maize auxin-binding protein together with homologous polypeptides in other species. We further find that the antibodies hyperpolarize protoplast transmembrane potential in an auxin-like manner. We conclude that these antibodies display auxin agonist activity and that we have identified an essential portion of the auxin-binding site.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>1323130</pmid><doi>10.1073/pnas.89.15.7208</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0002-4255-6440</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1992-08, Vol.89 (15), p.7208-7212 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_pnas_primary_89_15_7208 |
| source | Jstor Complete Legacy; MEDLINE; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | activity Agonists Amino Acid Sequence Amino acids Analytical, structural and metabolic biochemistry Antibodies ANTICORPS ANTICUERPOS Antiserum auxin-binding protein AUXINAS AUXINE Auxins Biological and medical sciences Cell membranes Cellular Biology Corn Fundamental and applied biological sciences. Psychology Indoleacetic Acids - metabolism Intracellular Membranes - drug effects Intracellular Membranes - physiology Life Sciences LIGAND LIGANDOS MEMBRANA MEMBRANE membrane potential Membrane Proteins - isolation & purification Membranes Microsomes - drug effects Microsomes - physiology Molecular Sequence Data Molecular Weight Naphthaleneacetic Acids - pharmacology Other biological molecules PEPTIDE Peptides - chemical synthesis Peptides - immunology PEPTIDOS Plant Growth Regulators Plant growth substances Plant Physiological Phenomena Plant Proteins Protein isoforms PROTEINAS PROTEINE Proteins Protoplasts Protoplasts - physiology Receptors Receptors, Cell Surface - immunology Receptors, Cell Surface - isolation & purification Receptors, Cell Surface - metabolism Space life sciences ZEA MAYS Zea mays - physiology |
| title | Antibodies to a peptide from the maize auxin-binding protein have auxin agonist activity |
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