The Large Form of Hepatitis δ Antigen is Crucial for Assembly of Hepatitis δ Virus
The virions of hepatitis δ virus (HDV) contain two species of HDV-specific protein, a large and a small form of hepatitis δ antigen (HDAg). We examined the role of individual HDAgs in virion assembly in cotransfection experiments. First, we constructed a replication-competent HDV mutant expressing o...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1991-10, Vol.88 (19), p.8490-8494 |
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| creator | Chang, Fu-Lin Chen, Pei-Jer Tu, Su-Jen Wang, Chuan-Jen Chen, Ding-Shinn |
| description | The virions of hepatitis δ virus (HDV) contain two species of HDV-specific protein, a large and a small form of hepatitis δ antigen (HDAg). We examined the role of individual HDAgs in virion assembly in cotransfection experiments. First, we constructed a replication-competent HDV mutant expressing only the small HDAg. When cotransfected with a plasmid expressing hepatitis B virus surface antigens to the HuH-7 cells, the mutant did not produce HDV virions, whereas the wild-type HDV clone did. Therefore, though the small HDAg is important for viral replication and is incorporated into the virus, the small-form δ antigen by itself is insufficient for virion formation. When the system was co-transfected with an additional plasmid providing the large HDAg, the HDV virion was then recovered. There was also evidence suggesting that the large HDAg could be copackaged into the HBsAg particles, without the presence of the HDV genome and the small HDAg. The results indicate a crucial role of the large HDAg in HDV assembly. |
| doi_str_mv | 10.1073/pnas.88.19.8490 |
| format | Article |
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We examined the role of individual HDAgs in virion assembly in cotransfection experiments. First, we constructed a replication-competent HDV mutant expressing only the small HDAg. When cotransfected with a plasmid expressing hepatitis B virus surface antigens to the HuH-7 cells, the mutant did not produce HDV virions, whereas the wild-type HDV clone did. Therefore, though the small HDAg is important for viral replication and is incorporated into the virus, the small-form δ antigen by itself is insufficient for virion formation. When the system was co-transfected with an additional plasmid providing the large HDAg, the HDV virion was then recovered. There was also evidence suggesting that the large HDAg could be copackaged into the HBsAg particles, without the presence of the HDV genome and the small HDAg. The results indicate a crucial role of the large HDAg in HDV assembly.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.88.19.8490</identifier><identifier>PMID: 1924308</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Antigens, Viral - physiology ; Base Sequence ; Biological and medical sciences ; Cell Line ; Cell lines ; Cloning, Molecular ; DNA Mutational Analysis ; Fundamental and applied biological sciences. Psychology ; Genomes ; Hepatitis ; Hepatitis antigens ; Hepatitis B Surface Antigens - metabolism ; Hepatitis B virus ; Hepatitis delta Antigens ; Hepatitis delta virus ; Hepatitis Delta Virus - growth & development ; Hepatitis Delta Virus - immunology ; Hepatitis Delta Virus - ultrastructure ; Humans ; In Vitro Techniques ; Microbiology ; Molecular Sequence Data ; Morphogenesis ; Oligonucleotides - chemistry ; Open reading frames ; Plasmids ; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains ; RNA ; RNA, Viral - metabolism ; Structure-Activity Relationship ; Transfection ; Virion - ultrastructure ; Virions ; Virology ; Virus Replication</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1991-10, Vol.88 (19), p.8490-8494</ispartof><rights>Copyright 1991 The National Academy of Sciences of the United States of America</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c588t-22d36c945f329b269eeadd312390201f4f83a2604bdb14b4cb22d19a5b02ee683</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/88/19.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2357965$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2357965$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27903,27904,53769,53771,57995,58228</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5001934$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1924308$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chang, Fu-Lin</creatorcontrib><creatorcontrib>Chen, Pei-Jer</creatorcontrib><creatorcontrib>Tu, Su-Jen</creatorcontrib><creatorcontrib>Wang, Chuan-Jen</creatorcontrib><creatorcontrib>Chen, Ding-Shinn</creatorcontrib><title>The Large Form of Hepatitis δ Antigen is Crucial for Assembly of Hepatitis δ Virus</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The virions of hepatitis δ virus (HDV) contain two species of HDV-specific protein, a large and a small form of hepatitis δ antigen (HDAg). We examined the role of individual HDAgs in virion assembly in cotransfection experiments. First, we constructed a replication-competent HDV mutant expressing only the small HDAg. When cotransfected with a plasmid expressing hepatitis B virus surface antigens to the HuH-7 cells, the mutant did not produce HDV virions, whereas the wild-type HDV clone did. Therefore, though the small HDAg is important for viral replication and is incorporated into the virus, the small-form δ antigen by itself is insufficient for virion formation. When the system was co-transfected with an additional plasmid providing the large HDAg, the HDV virion was then recovered. There was also evidence suggesting that the large HDAg could be copackaged into the HBsAg particles, without the presence of the HDV genome and the small HDAg. The results indicate a crucial role of the large HDAg in HDV assembly.</description><subject>Antigens, Viral - physiology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Cell lines</subject><subject>Cloning, Molecular</subject><subject>DNA Mutational Analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genomes</subject><subject>Hepatitis</subject><subject>Hepatitis antigens</subject><subject>Hepatitis B Surface Antigens - metabolism</subject><subject>Hepatitis B virus</subject><subject>Hepatitis delta Antigens</subject><subject>Hepatitis delta virus</subject><subject>Hepatitis Delta Virus - growth & development</subject><subject>Hepatitis Delta Virus - immunology</subject><subject>Hepatitis Delta Virus - ultrastructure</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Morphogenesis</subject><subject>Oligonucleotides - chemistry</subject><subject>Open reading frames</subject><subject>Plasmids</subject><subject>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</subject><subject>RNA</subject><subject>RNA, Viral - metabolism</subject><subject>Structure-Activity Relationship</subject><subject>Transfection</subject><subject>Virion - ultrastructure</subject><subject>Virions</subject><subject>Virology</subject><subject>Virus Replication</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1uEzEUhS1EVUJhzQaQF4iuJr3-m9gSmyiiP1IkNoGt5fF4Ulcz42DPVPS9eA6eCY8SUhASrK6vznfu9dVB6BWBOYEFu9j1Js2lnBM1l1zBEzQjoEhR5vdTNAOgi0Jyyp-h5yndAYASEk7RKVGUM5AztNncOrw2cevwZYgdDg2-djsz-MEn_OM7XvaD37oe524VR-tNi5sQ8TIl11Xtw1_8Fx_H9AKdNKZN7uWhnqHPlx83q-ti_enqZrVcF1ZIORSU1qy0iouGUVXRUjln6poRyhRQIA1vJDO0BF7VFeEVt1V2EGVEBdS5UrIz9GE_dzdWnaut64doWr2LvjPxQQfj9Z9K72_1NtxrQQXj2f7-YI_h6-jSoDufrGtb07swJr2ghCsF8F-QlEQxKssMXuxBG0NK0TXHvxDQU156yktLqYnSU17Z8eb3Ex75fUBZf3fQTbKmbaLprU9HTADk1dMp5wdsmv9Lfdyjm7FtB_dtyOTbf5IZeL0H7tIQ4pGgTCxUKdhPDbjAvw</recordid><startdate>19911001</startdate><enddate>19911001</enddate><creator>Chang, Fu-Lin</creator><creator>Chen, Pei-Jer</creator><creator>Tu, Su-Jen</creator><creator>Wang, Chuan-Jen</creator><creator>Chen, Ding-Shinn</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19911001</creationdate><title>The Large Form of Hepatitis δ Antigen is Crucial for Assembly of Hepatitis δ Virus</title><author>Chang, Fu-Lin ; Chen, Pei-Jer ; Tu, Su-Jen ; Wang, Chuan-Jen ; Chen, Ding-Shinn</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c588t-22d36c945f329b269eeadd312390201f4f83a2604bdb14b4cb22d19a5b02ee683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Antigens, Viral - physiology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Cell lines</topic><topic>Cloning, Molecular</topic><topic>DNA Mutational Analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genomes</topic><topic>Hepatitis</topic><topic>Hepatitis antigens</topic><topic>Hepatitis B Surface Antigens - metabolism</topic><topic>Hepatitis B virus</topic><topic>Hepatitis delta Antigens</topic><topic>Hepatitis delta virus</topic><topic>Hepatitis Delta Virus - growth & development</topic><topic>Hepatitis Delta Virus - immunology</topic><topic>Hepatitis Delta Virus - ultrastructure</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Morphogenesis</topic><topic>Oligonucleotides - chemistry</topic><topic>Open reading frames</topic><topic>Plasmids</topic><topic>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</topic><topic>RNA</topic><topic>RNA, Viral - metabolism</topic><topic>Structure-Activity Relationship</topic><topic>Transfection</topic><topic>Virion - ultrastructure</topic><topic>Virions</topic><topic>Virology</topic><topic>Virus Replication</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chang, Fu-Lin</creatorcontrib><creatorcontrib>Chen, Pei-Jer</creatorcontrib><creatorcontrib>Tu, Su-Jen</creatorcontrib><creatorcontrib>Wang, Chuan-Jen</creatorcontrib><creatorcontrib>Chen, Ding-Shinn</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chang, Fu-Lin</au><au>Chen, Pei-Jer</au><au>Tu, Su-Jen</au><au>Wang, Chuan-Jen</au><au>Chen, Ding-Shinn</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Large Form of Hepatitis δ Antigen is Crucial for Assembly of Hepatitis δ Virus</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1991-10-01</date><risdate>1991</risdate><volume>88</volume><issue>19</issue><spage>8490</spage><epage>8494</epage><pages>8490-8494</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>The virions of hepatitis δ virus (HDV) contain two species of HDV-specific protein, a large and a small form of hepatitis δ antigen (HDAg). We examined the role of individual HDAgs in virion assembly in cotransfection experiments. First, we constructed a replication-competent HDV mutant expressing only the small HDAg. When cotransfected with a plasmid expressing hepatitis B virus surface antigens to the HuH-7 cells, the mutant did not produce HDV virions, whereas the wild-type HDV clone did. Therefore, though the small HDAg is important for viral replication and is incorporated into the virus, the small-form δ antigen by itself is insufficient for virion formation. When the system was co-transfected with an additional plasmid providing the large HDAg, the HDV virion was then recovered. There was also evidence suggesting that the large HDAg could be copackaged into the HBsAg particles, without the presence of the HDV genome and the small HDAg. The results indicate a crucial role of the large HDAg in HDV assembly.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>1924308</pmid><doi>10.1073/pnas.88.19.8490</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1991-10, Vol.88 (19), p.8490-8494 |
| issn | 0027-8424 1091-6490 |
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| subjects | Antigens, Viral - physiology Base Sequence Biological and medical sciences Cell Line Cell lines Cloning, Molecular DNA Mutational Analysis Fundamental and applied biological sciences. Psychology Genomes Hepatitis Hepatitis antigens Hepatitis B Surface Antigens - metabolism Hepatitis B virus Hepatitis delta Antigens Hepatitis delta virus Hepatitis Delta Virus - growth & development Hepatitis Delta Virus - immunology Hepatitis Delta Virus - ultrastructure Humans In Vitro Techniques Microbiology Molecular Sequence Data Morphogenesis Oligonucleotides - chemistry Open reading frames Plasmids Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains RNA RNA, Viral - metabolism Structure-Activity Relationship Transfection Virion - ultrastructure Virions Virology Virus Replication |
| title | The Large Form of Hepatitis δ Antigen is Crucial for Assembly of Hepatitis δ Virus |
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