Production of Platelet-Derived Endothelial Cell Growth Factor by Normal and Transformed Human Cells in Culture
Platelet-derived endothelial cell growth factor (PD-ECGF) is a 45-kDa endothelial cell mitogen which has angiogenic properties in vivo. We report here that human foreskin fibroblasts, a human squamous cell carcinoma cell line, and 2 out of the 3 human thyroid carcinoma cell lines investigated produc...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1989-10, Vol.86 (19), p.7427-7431 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Usuki, Kensuke Heldin, Nils-Erik Miyazono, Kohei Ishikawa, Fuyuki Takaku, Fumimaro Westermark, Bengt Heldin, Carl-Henrik |
| description | Platelet-derived endothelial cell growth factor (PD-ECGF) is a 45-kDa endothelial cell mitogen which has angiogenic properties in vivo. We report here that human foreskin fibroblasts, a human squamous cell carcinoma cell line, and 2 out of the 3 human thyroid carcinoma cell lines investigated produce PD-ECGF, whereas 21 other cell lines examined do not. The positive cell lines contained a 1.8-kilobase PD-ECGF mRNA, and a 45-kDa protein could be demonstrated in lysates of the cell lines by immunoblotting and immunoprecipitation using a specific antiserum against PD-ECGF. Furthermore, the cell lysates contained mitogenic activity for endothelial cells that was neutralized by the PD-ECGF antiserum. PD-ECGF was found to be secreted only slowly from the producer cells, consistent with the previous finding that the primary translation product lacks a signal sequence. The restricted expression and intracellular sequestration of PD-ECGF imply a strictly controlled function in endothelial cell proliferation and angiogenesis. Aberrant production of PD-ECGF may play a role in tumor angiogenesis. |
| doi_str_mv | 10.1073/pnas.86.19.7427 |
| format | Article |
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We report here that human foreskin fibroblasts, a human squamous cell carcinoma cell line, and 2 out of the 3 human thyroid carcinoma cell lines investigated produce PD-ECGF, whereas 21 other cell lines examined do not. The positive cell lines contained a 1.8-kilobase PD-ECGF mRNA, and a 45-kDa protein could be demonstrated in lysates of the cell lines by immunoblotting and immunoprecipitation using a specific antiserum against PD-ECGF. Furthermore, the cell lysates contained mitogenic activity for endothelial cells that was neutralized by the PD-ECGF antiserum. PD-ECGF was found to be secreted only slowly from the producer cells, consistent with the previous finding that the primary translation product lacks a signal sequence. The restricted expression and intracellular sequestration of PD-ECGF imply a strictly controlled function in endothelial cell proliferation and angiogenesis. Aberrant production of PD-ECGF may play a role in tumor angiogenesis.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.86.19.7427</identifier><identifier>PMID: 2678104</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Analytical, structural and metabolic biochemistry ; Animals ; Antiserum ; Biological and medical sciences ; Blood Platelets - metabolism ; Cell Division - drug effects ; Cell growth ; Cell Line ; Cell lines ; Cell Transformation, Neoplastic ; Cells ; Cells, Cultured ; Endothelial cells ; Endothelial Growth Factors ; Endothelium, Vascular - cytology ; Endothelium, Vascular - drug effects ; Endothelium, Vascular - physiology ; Fibroblasts ; Foreskin ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Growth Substances - biosynthesis ; Growth Substances - genetics ; Growth Substances - pharmacology ; Humans ; Kinetics ; Male ; Protein hormones. Growth factors. Cytokines ; Proteins ; Skin - metabolism ; Squamous cell carcinoma ; Swine ; Thyroid cancer ; Transcription, Genetic ; Tumor cell line</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1989-10, Vol.86 (19), p.7427-7431</ispartof><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c522t-6b8ac957594370c7965f9ae087782b5ea1183be2e0b71540a9ab3034c5a8a1753</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/86/19.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/34659$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/34659$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19351507$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2678104$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Usuki, Kensuke</creatorcontrib><creatorcontrib>Heldin, Nils-Erik</creatorcontrib><creatorcontrib>Miyazono, Kohei</creatorcontrib><creatorcontrib>Ishikawa, Fuyuki</creatorcontrib><creatorcontrib>Takaku, Fumimaro</creatorcontrib><creatorcontrib>Westermark, Bengt</creatorcontrib><creatorcontrib>Heldin, Carl-Henrik</creatorcontrib><title>Production of Platelet-Derived Endothelial Cell Growth Factor by Normal and Transformed Human Cells in Culture</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Platelet-derived endothelial cell growth factor (PD-ECGF) is a 45-kDa endothelial cell mitogen which has angiogenic properties in vivo. We report here that human foreskin fibroblasts, a human squamous cell carcinoma cell line, and 2 out of the 3 human thyroid carcinoma cell lines investigated produce PD-ECGF, whereas 21 other cell lines examined do not. The positive cell lines contained a 1.8-kilobase PD-ECGF mRNA, and a 45-kDa protein could be demonstrated in lysates of the cell lines by immunoblotting and immunoprecipitation using a specific antiserum against PD-ECGF. Furthermore, the cell lysates contained mitogenic activity for endothelial cells that was neutralized by the PD-ECGF antiserum. PD-ECGF was found to be secreted only slowly from the producer cells, consistent with the previous finding that the primary translation product lacks a signal sequence. The restricted expression and intracellular sequestration of PD-ECGF imply a strictly controlled function in endothelial cell proliferation and angiogenesis. Aberrant production of PD-ECGF may play a role in tumor angiogenesis.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Antiserum</subject><subject>Biological and medical sciences</subject><subject>Blood Platelets - metabolism</subject><subject>Cell Division - drug effects</subject><subject>Cell growth</subject><subject>Cell Line</subject><subject>Cell lines</subject><subject>Cell Transformation, Neoplastic</subject><subject>Cells</subject><subject>Cells, Cultured</subject><subject>Endothelial cells</subject><subject>Endothelial Growth Factors</subject><subject>Endothelium, Vascular - cytology</subject><subject>Endothelium, Vascular - drug effects</subject><subject>Endothelium, Vascular - physiology</subject><subject>Fibroblasts</subject><subject>Foreskin</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Growth Substances - biosynthesis</subject><subject>Growth Substances - genetics</subject><subject>Growth Substances - pharmacology</subject><subject>Humans</subject><subject>Kinetics</subject><subject>Male</subject><subject>Protein hormones. Growth factors. Cytokines</subject><subject>Proteins</subject><subject>Skin - metabolism</subject><subject>Squamous cell carcinoma</subject><subject>Swine</subject><subject>Thyroid cancer</subject><subject>Transcription, Genetic</subject><subject>Tumor cell line</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtvEzEUhS1EVUJgjYQE8ga6mtSP8dhesEChD6QKuihr647jIVM542B7Cv33eEhIYQMr2zrfuQ8fhF5QsqBE8tPtAGmhmgXVC1kz-QjNKNG0ampNHqMZIUxWqmb1E_Q0pVtCiBaKHKNj1khFST1Dw3UMq9HmPgw4dPjaQ3be5eqDi_2dW-GzYRXy2vkePF467_FFDN_zGp-DzSHi9h5_CnFTRBhW-CbCkLryLsbLcQPDL0vCfbmMPo_RPUNHHfjknu_POfpyfnazvKyuPl98XL6_qqxgLFdNq8BqIYWuuSRW6kZ0GhxRUirWCgeUKt465kgrqagJaGg54bUVoIBKwefo3a7udmzLNNYNOYI329hvIN6bAL35Wxn6tfka7gzTikhZ_G_3_hi-jS5ls-mTLcvA4MKYjNRMsEb_H6SCy0aWNebodAfaGFKKrjsMQ4mZojRTlEY1hmozRVkcr_7c4cDvsyv6m70OyYLvyufbPj2U1VxQQaY6J3tuavBbfmhkutH77H7kQr7-J1mAlzvgNpX0DwSvG6H5TyLuyVk</recordid><startdate>19891001</startdate><enddate>19891001</enddate><creator>Usuki, Kensuke</creator><creator>Heldin, Nils-Erik</creator><creator>Miyazono, Kohei</creator><creator>Ishikawa, Fuyuki</creator><creator>Takaku, Fumimaro</creator><creator>Westermark, Bengt</creator><creator>Heldin, Carl-Henrik</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19891001</creationdate><title>Production of Platelet-Derived Endothelial Cell Growth Factor by Normal and Transformed Human Cells in Culture</title><author>Usuki, Kensuke ; Heldin, Nils-Erik ; Miyazono, Kohei ; Ishikawa, Fuyuki ; Takaku, Fumimaro ; Westermark, Bengt ; Heldin, Carl-Henrik</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c522t-6b8ac957594370c7965f9ae087782b5ea1183be2e0b71540a9ab3034c5a8a1753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Antiserum</topic><topic>Biological and medical sciences</topic><topic>Blood Platelets - metabolism</topic><topic>Cell Division - drug effects</topic><topic>Cell growth</topic><topic>Cell Line</topic><topic>Cell lines</topic><topic>Cell Transformation, Neoplastic</topic><topic>Cells</topic><topic>Cells, Cultured</topic><topic>Endothelial cells</topic><topic>Endothelial Growth Factors</topic><topic>Endothelium, Vascular - cytology</topic><topic>Endothelium, Vascular - drug effects</topic><topic>Endothelium, Vascular - physiology</topic><topic>Fibroblasts</topic><topic>Foreskin</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Growth Substances - biosynthesis</topic><topic>Growth Substances - genetics</topic><topic>Growth Substances - pharmacology</topic><topic>Humans</topic><topic>Kinetics</topic><topic>Male</topic><topic>Protein hormones. Growth factors. Cytokines</topic><topic>Proteins</topic><topic>Skin - metabolism</topic><topic>Squamous cell carcinoma</topic><topic>Swine</topic><topic>Thyroid cancer</topic><topic>Transcription, Genetic</topic><topic>Tumor cell line</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Usuki, Kensuke</creatorcontrib><creatorcontrib>Heldin, Nils-Erik</creatorcontrib><creatorcontrib>Miyazono, Kohei</creatorcontrib><creatorcontrib>Ishikawa, Fuyuki</creatorcontrib><creatorcontrib>Takaku, Fumimaro</creatorcontrib><creatorcontrib>Westermark, Bengt</creatorcontrib><creatorcontrib>Heldin, Carl-Henrik</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Usuki, Kensuke</au><au>Heldin, Nils-Erik</au><au>Miyazono, Kohei</au><au>Ishikawa, Fuyuki</au><au>Takaku, Fumimaro</au><au>Westermark, Bengt</au><au>Heldin, Carl-Henrik</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Production of Platelet-Derived Endothelial Cell Growth Factor by Normal and Transformed Human Cells in Culture</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1989-10-01</date><risdate>1989</risdate><volume>86</volume><issue>19</issue><spage>7427</spage><epage>7431</epage><pages>7427-7431</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>Platelet-derived endothelial cell growth factor (PD-ECGF) is a 45-kDa endothelial cell mitogen which has angiogenic properties in vivo. We report here that human foreskin fibroblasts, a human squamous cell carcinoma cell line, and 2 out of the 3 human thyroid carcinoma cell lines investigated produce PD-ECGF, whereas 21 other cell lines examined do not. The positive cell lines contained a 1.8-kilobase PD-ECGF mRNA, and a 45-kDa protein could be demonstrated in lysates of the cell lines by immunoblotting and immunoprecipitation using a specific antiserum against PD-ECGF. Furthermore, the cell lysates contained mitogenic activity for endothelial cells that was neutralized by the PD-ECGF antiserum. PD-ECGF was found to be secreted only slowly from the producer cells, consistent with the previous finding that the primary translation product lacks a signal sequence. The restricted expression and intracellular sequestration of PD-ECGF imply a strictly controlled function in endothelial cell proliferation and angiogenesis. Aberrant production of PD-ECGF may play a role in tumor angiogenesis.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>2678104</pmid><doi>10.1073/pnas.86.19.7427</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1989-10, Vol.86 (19), p.7427-7431 |
| issn | 0027-8424 1091-6490 |
| language | eng |
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| source | Jstor Complete Legacy; MEDLINE; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Analytical, structural and metabolic biochemistry Animals Antiserum Biological and medical sciences Blood Platelets - metabolism Cell Division - drug effects Cell growth Cell Line Cell lines Cell Transformation, Neoplastic Cells Cells, Cultured Endothelial cells Endothelial Growth Factors Endothelium, Vascular - cytology Endothelium, Vascular - drug effects Endothelium, Vascular - physiology Fibroblasts Foreskin Fundamental and applied biological sciences. Psychology Gene Expression Growth Substances - biosynthesis Growth Substances - genetics Growth Substances - pharmacology Humans Kinetics Male Protein hormones. Growth factors. Cytokines Proteins Skin - metabolism Squamous cell carcinoma Swine Thyroid cancer Transcription, Genetic Tumor cell line |
| title | Production of Platelet-Derived Endothelial Cell Growth Factor by Normal and Transformed Human Cells in Culture |
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