A 60-kDa Cytoskeletal Protein from Trypanosoma brucei brucei Can Interact with Membranes and with Microtubules
The cytoskeleton of eukaryotic cells is a major determinant of cellular architecture and of many cellular functions. In addition to or in place of the transcellular cytoskeleton, many eukaryotic cells also contain membrane-associated cytoskeletal structures (membrane skeletons), which are important...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1988-02, Vol.85 (4), p.1101-1104 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Seebeck, Thomas Küng, Valentin Wyler, Toni Müller, Michele |
| description | The cytoskeleton of eukaryotic cells is a major determinant of cellular architecture and of many cellular functions. In addition to or in place of the transcellular cytoskeleton, many eukaryotic cells also contain membrane-associated cytoskeletal structures (membrane skeletons), which are important for cellular structure and function. The membrane skeleton of the parasitic hemoflagellate Trypanosoma brucei consists of a dense array of singlet microtubules (subpellicular microtubules), which are tightly associated to the overlying cell membrane. This study reports the identification of a microtubule-associated protein from Trypanosoma brucei that constitutes a component of the link between this microtubular array and the cell membrane. The protein can bind in vitro both to microtubules and to membrane vesicles or liposomes. Furthermore, it can crosslink microtubules and membrane vesicles, suggesting that it exerts a similar function in the membrane skeleton. |
| doi_str_mv | 10.1073/pnas.85.4.1101 |
| format | Article |
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Psychology ; Gels ; Human protozoal diseases ; Infectious diseases ; Liposomes ; Liposomes - metabolism ; Medical sciences ; Microtubule associated proteins ; Microtubule-Associated Proteins - metabolism ; Microtubules ; Microtubules - metabolism ; Molecular and cellular biology ; P branes ; Parasitic diseases ; Parasitism ; Protozoal diseases ; Skeleton ; Tropical medicine ; Trypanosoma brucei brucei ; Trypanosoma brucei brucei - metabolism ; Trypanosomiasis ; Tubulin - metabolism</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1988-02, Vol.85 (4), p.1101-1104</ispartof><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c462t-8b6c9b0e6de7266fe268011d019c1e8fe65a54ec6746202efaf809895a58e67a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/85/4.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/30945$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/30945$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7629297$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3422481$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Seebeck, Thomas</creatorcontrib><creatorcontrib>Küng, Valentin</creatorcontrib><creatorcontrib>Wyler, Toni</creatorcontrib><creatorcontrib>Müller, Michele</creatorcontrib><title>A 60-kDa Cytoskeletal Protein from Trypanosoma brucei brucei Can Interact with Membranes and with Microtubules</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The cytoskeleton of eukaryotic cells is a major determinant of cellular architecture and of many cellular functions. In addition to or in place of the transcellular cytoskeleton, many eukaryotic cells also contain membrane-associated cytoskeletal structures (membrane skeletons), which are important for cellular structure and function. The membrane skeleton of the parasitic hemoflagellate Trypanosoma brucei consists of a dense array of singlet microtubules (subpellicular microtubules), which are tightly associated to the overlying cell membrane. This study reports the identification of a microtubule-associated protein from Trypanosoma brucei that constitutes a component of the link between this microtubular array and the cell membrane. The protein can bind in vitro both to microtubules and to membrane vesicles or liposomes. Furthermore, it can crosslink microtubules and membrane vesicles, suggesting that it exerts a similar function in the membrane skeleton.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Membrane - metabolism</subject><subject>Cell membranes</subject><subject>Cell nucleus</subject><subject>Cell structures and functions</subject><subject>Cytoskeletal Proteins - metabolism</subject><subject>Cytoskeleton</subject><subject>Erythrocyte membrane</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gels</subject><subject>Human protozoal diseases</subject><subject>Infectious diseases</subject><subject>Liposomes</subject><subject>Liposomes - metabolism</subject><subject>Medical sciences</subject><subject>Microtubule associated proteins</subject><subject>Microtubule-Associated Proteins - metabolism</subject><subject>Microtubules</subject><subject>Microtubules - metabolism</subject><subject>Molecular and cellular biology</subject><subject>P branes</subject><subject>Parasitic diseases</subject><subject>Parasitism</subject><subject>Protozoal diseases</subject><subject>Skeleton</subject><subject>Tropical medicine</subject><subject>Trypanosoma brucei brucei</subject><subject>Trypanosoma brucei brucei - metabolism</subject><subject>Trypanosomiasis</subject><subject>Tubulin - metabolism</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkb1vFDEQxS0ECkegpUBCcoHS7TL2ef1RRgeBSEFQhNry-sZik_04bK_g_nt8us2RAolqpHk_v3meIeQ1g5qBWr_fjS7VuqlFzRiwJ2TFwLBKCgNPyQqAq0oLLp6TFyndAYBpNJyRs7XgXGi2IuMllVDdf3B0s89Tusces-vptzhl7EYa4jTQ27jfuXFK0-BoG2eP3UPZuJFejxmj85n-6vIP-gWHNroRE3Xjdml1vrjN7dxjekmeBdcnfLXUc_L96uPt5nN18_XT9ebypvJC8lzpVnrTAsotKi5lQC41MLYFZjxDHVA2rhHopSo4cAwuaDDalK5Gqdz6nFwcfXdx-jljynbokse-L9GmOVmlQStmzH9BJrRSQusC1kewfCaliMHuYje4uLcM7OES9nAJqxsr7OES5cHbxXluB9ye8GX1RX-36C5514eyNd-lE6YkN9yoRwEP9g_qaYwNc99n_J0fzfsnWPQ3R_0u5Sn-jQNGNOs_X8GxkQ</recordid><startdate>19880201</startdate><enddate>19880201</enddate><creator>Seebeck, Thomas</creator><creator>Küng, Valentin</creator><creator>Wyler, Toni</creator><creator>Müller, Michele</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>19880201</creationdate><title>A 60-kDa Cytoskeletal Protein from Trypanosoma brucei brucei Can Interact with Membranes and with Microtubules</title><author>Seebeck, Thomas ; Küng, Valentin ; Wyler, Toni ; Müller, Michele</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c462t-8b6c9b0e6de7266fe268011d019c1e8fe65a54ec6746202efaf809895a58e67a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Membrane - metabolism</topic><topic>Cell membranes</topic><topic>Cell nucleus</topic><topic>Cell structures and functions</topic><topic>Cytoskeletal Proteins - metabolism</topic><topic>Cytoskeleton</topic><topic>Erythrocyte membrane</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gels</topic><topic>Human protozoal diseases</topic><topic>Infectious diseases</topic><topic>Liposomes</topic><topic>Liposomes - metabolism</topic><topic>Medical sciences</topic><topic>Microtubule associated proteins</topic><topic>Microtubule-Associated Proteins - metabolism</topic><topic>Microtubules</topic><topic>Microtubules - metabolism</topic><topic>Molecular and cellular biology</topic><topic>P branes</topic><topic>Parasitic diseases</topic><topic>Parasitism</topic><topic>Protozoal diseases</topic><topic>Skeleton</topic><topic>Tropical medicine</topic><topic>Trypanosoma brucei brucei</topic><topic>Trypanosoma brucei brucei - metabolism</topic><topic>Trypanosomiasis</topic><topic>Tubulin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Seebeck, Thomas</creatorcontrib><creatorcontrib>Küng, Valentin</creatorcontrib><creatorcontrib>Wyler, Toni</creatorcontrib><creatorcontrib>Müller, Michele</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Seebeck, Thomas</au><au>Küng, Valentin</au><au>Wyler, Toni</au><au>Müller, Michele</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A 60-kDa Cytoskeletal Protein from Trypanosoma brucei brucei Can Interact with Membranes and with Microtubules</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1988-02-01</date><risdate>1988</risdate><volume>85</volume><issue>4</issue><spage>1101</spage><epage>1104</epage><pages>1101-1104</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>The cytoskeleton of eukaryotic cells is a major determinant of cellular architecture and of many cellular functions. In addition to or in place of the transcellular cytoskeleton, many eukaryotic cells also contain membrane-associated cytoskeletal structures (membrane skeletons), which are important for cellular structure and function. The membrane skeleton of the parasitic hemoflagellate Trypanosoma brucei consists of a dense array of singlet microtubules (subpellicular microtubules), which are tightly associated to the overlying cell membrane. This study reports the identification of a microtubule-associated protein from Trypanosoma brucei that constitutes a component of the link between this microtubular array and the cell membrane. The protein can bind in vitro both to microtubules and to membrane vesicles or liposomes. Furthermore, it can crosslink microtubules and membrane vesicles, suggesting that it exerts a similar function in the membrane skeleton.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>3422481</pmid><doi>10.1073/pnas.85.4.1101</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1988-02, Vol.85 (4), p.1101-1104 |
| issn | 0027-8424 1091-6490 |
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| source | Jstor Complete Legacy; MEDLINE; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Animals Biological and medical sciences Cell Membrane - metabolism Cell membranes Cell nucleus Cell structures and functions Cytoskeletal Proteins - metabolism Cytoskeleton Erythrocyte membrane Fundamental and applied biological sciences. Psychology Gels Human protozoal diseases Infectious diseases Liposomes Liposomes - metabolism Medical sciences Microtubule associated proteins Microtubule-Associated Proteins - metabolism Microtubules Microtubules - metabolism Molecular and cellular biology P branes Parasitic diseases Parasitism Protozoal diseases Skeleton Tropical medicine Trypanosoma brucei brucei Trypanosoma brucei brucei - metabolism Trypanosomiasis Tubulin - metabolism |
| title | A 60-kDa Cytoskeletal Protein from Trypanosoma brucei brucei Can Interact with Membranes and with Microtubules |
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