Purification and Characterization of Other Distinct Bone-Inducing Factors
We purified a factor that induces bone formation >300,000-fold from guanidinium chloride extracts of demineralized bone. Fifty nanograms of highly purified protein was active in an in vivo cartilage and bone-formation assay. The activity resided in a single gel band, corresponding to a molecular...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1988-12, Vol.85 (24), p.9484-9488 |
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| container_end_page | 9488 |
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| container_issue | 24 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 85 |
| creator | Wang, Elizabeth A. Rosen, Vicki Cordes, Paul Hewick, Rodney M. Kriz, Mary Jo Luxenberg, Deborah P. Sibley, Barbara S. Wozney, John M. |
| description | We purified a factor that induces bone formation >300,000-fold from guanidinium chloride extracts of demineralized bone. Fifty nanograms of highly purified protein was active in an in vivo cartilage and bone-formation assay. The activity resided in a single gel band, corresponding to a molecular mass of ≈ 30 kDa, which yielded proteins of 30, 18, and 16 kDa on reduction. The partial amino acid sequence obtained from these proteins confirmed our identification of specific factors that induce new bone formation in vivo. |
| doi_str_mv | 10.1073/pnas.85.24.9484 |
| format | Article |
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Fifty nanograms of highly purified protein was active in an in vivo cartilage and bone-formation assay. The activity resided in a single gel band, corresponding to a molecular mass of ≈ 30 kDa, which yielded proteins of 30, 18, and 16 kDa on reduction. The partial amino acid sequence obtained from these proteins confirmed our identification of specific factors that induce new bone formation in vivo.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.85.24.9484</identifier><identifier>PMID: 3200834</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Amino Acid Sequence ; Amino acids ; Analytical, structural and metabolic biochemistry ; Animals ; Biological and medical sciences ; Bone formation ; bone morphogenetic protein ; Bone Morphogenetic Proteins ; Bones ; Cartilage ; Cattle ; Chlorides ; Fibroblast growth factors ; Fractionation ; Fundamental and applied biological sciences. Psychology ; Gels ; Human growth ; Molecular Weight ; Osteogenesis ; Protein hormones. Growth factors. Cytokines ; Proteins ; Proteins - analysis ; Proteins - isolation & purification ; Rats ; Ungulates</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1988-12, Vol.85 (24), p.9484-9488</ispartof><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c533t-5040eb53a2bdb494467cf2d41a2c8aaa639c8cf212849680f2e203e07c675f613</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/85/24.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/32658$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/32658$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6602494$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3200834$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Elizabeth A.</creatorcontrib><creatorcontrib>Rosen, Vicki</creatorcontrib><creatorcontrib>Cordes, Paul</creatorcontrib><creatorcontrib>Hewick, Rodney M.</creatorcontrib><creatorcontrib>Kriz, Mary Jo</creatorcontrib><creatorcontrib>Luxenberg, Deborah P.</creatorcontrib><creatorcontrib>Sibley, Barbara S.</creatorcontrib><creatorcontrib>Wozney, John M.</creatorcontrib><title>Purification and Characterization of Other Distinct Bone-Inducing Factors</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>We purified a factor that induces bone formation >300,000-fold from guanidinium chloride extracts of demineralized bone. Fifty nanograms of highly purified protein was active in an in vivo cartilage and bone-formation assay. The activity resided in a single gel band, corresponding to a molecular mass of ≈ 30 kDa, which yielded proteins of 30, 18, and 16 kDa on reduction. The partial amino acid sequence obtained from these proteins confirmed our identification of specific factors that induce new bone formation in vivo.</description><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Bone formation</subject><subject>bone morphogenetic protein</subject><subject>Bone Morphogenetic Proteins</subject><subject>Bones</subject><subject>Cartilage</subject><subject>Cattle</subject><subject>Chlorides</subject><subject>Fibroblast growth factors</subject><subject>Fractionation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gels</subject><subject>Human growth</subject><subject>Molecular Weight</subject><subject>Osteogenesis</subject><subject>Protein hormones. Growth factors. Cytokines</subject><subject>Proteins</subject><subject>Proteins - analysis</subject><subject>Proteins - isolation & purification</subject><subject>Rats</subject><subject>Ungulates</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0c9vFCEUB3BiNHWtnk1MNHMweprt4zccdbW6SZN60DNhGbA0s8wKTKL-9bLZsXrSE8l7Hx7wBaGnGNYYJL04JFvWiq8JW2um2D20wqBxL5iG-2gFQGSvGGEP0aNSbgFAcwVn6IwSAEXZCm0_zTmG6GyNU-psGrrNjc3WVZ_jz1NxCt11vfG5exdLjcnV7u2UfL9Nw-xi-tpdNj3l8hg9CHYs_smynqMvl-8_bz72V9cftps3V73jlNaeAwO_49SS3bBjmjEhXSADw5Y4Za0VVDvVKpgopoWCQDwB6kE6IXkQmJ6jV6e5hzx9m32pZh-L8-Nok5_mYqTiklOp_gsxB0mI1g1enKDLUynZB3PIcW_zD4PBHFM2x5SN4oYwc0y57Xi-jJ53ez_c-SXW1n-59G1xdgzZJhfLHRMCSHt6Y68Xdpz_u_vnHBPmcaz-e23yxT9lA89O4La0z_jrQoIr-gvtDqUn</recordid><startdate>19881201</startdate><enddate>19881201</enddate><creator>Wang, Elizabeth A.</creator><creator>Rosen, Vicki</creator><creator>Cordes, Paul</creator><creator>Hewick, Rodney M.</creator><creator>Kriz, Mary Jo</creator><creator>Luxenberg, Deborah P.</creator><creator>Sibley, Barbara S.</creator><creator>Wozney, John M.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M81</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19881201</creationdate><title>Purification and Characterization of Other Distinct Bone-Inducing Factors</title><author>Wang, Elizabeth A. ; Rosen, Vicki ; Cordes, Paul ; Hewick, Rodney M. ; Kriz, Mary Jo ; Luxenberg, Deborah P. ; Sibley, Barbara S. ; Wozney, John M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c533t-5040eb53a2bdb494467cf2d41a2c8aaa639c8cf212849680f2e203e07c675f613</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Bone formation</topic><topic>bone morphogenetic protein</topic><topic>Bone Morphogenetic Proteins</topic><topic>Bones</topic><topic>Cartilage</topic><topic>Cattle</topic><topic>Chlorides</topic><topic>Fibroblast growth factors</topic><topic>Fractionation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gels</topic><topic>Human growth</topic><topic>Molecular Weight</topic><topic>Osteogenesis</topic><topic>Protein hormones. Growth factors. Cytokines</topic><topic>Proteins</topic><topic>Proteins - analysis</topic><topic>Proteins - isolation & purification</topic><topic>Rats</topic><topic>Ungulates</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Elizabeth A.</creatorcontrib><creatorcontrib>Rosen, Vicki</creatorcontrib><creatorcontrib>Cordes, Paul</creatorcontrib><creatorcontrib>Hewick, Rodney M.</creatorcontrib><creatorcontrib>Kriz, Mary Jo</creatorcontrib><creatorcontrib>Luxenberg, Deborah P.</creatorcontrib><creatorcontrib>Sibley, Barbara S.</creatorcontrib><creatorcontrib>Wozney, John M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Elizabeth A.</au><au>Rosen, Vicki</au><au>Cordes, Paul</au><au>Hewick, Rodney M.</au><au>Kriz, Mary Jo</au><au>Luxenberg, Deborah P.</au><au>Sibley, Barbara S.</au><au>Wozney, John M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Purification and Characterization of Other Distinct Bone-Inducing Factors</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1988-12-01</date><risdate>1988</risdate><volume>85</volume><issue>24</issue><spage>9484</spage><epage>9488</epage><pages>9484-9488</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>We purified a factor that induces bone formation >300,000-fold from guanidinium chloride extracts of demineralized bone. Fifty nanograms of highly purified protein was active in an in vivo cartilage and bone-formation assay. The activity resided in a single gel band, corresponding to a molecular mass of ≈ 30 kDa, which yielded proteins of 30, 18, and 16 kDa on reduction. The partial amino acid sequence obtained from these proteins confirmed our identification of specific factors that induce new bone formation in vivo.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>3200834</pmid><doi>10.1073/pnas.85.24.9484</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1988-12, Vol.85 (24), p.9484-9488 |
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| language | eng |
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| source | Jstor Complete Legacy; MEDLINE; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Amino Acid Sequence Amino acids Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Bone formation bone morphogenetic protein Bone Morphogenetic Proteins Bones Cartilage Cattle Chlorides Fibroblast growth factors Fractionation Fundamental and applied biological sciences. Psychology Gels Human growth Molecular Weight Osteogenesis Protein hormones. Growth factors. Cytokines Proteins Proteins - analysis Proteins - isolation & purification Rats Ungulates |
| title | Purification and Characterization of Other Distinct Bone-Inducing Factors |
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