Frequency and Spectrum of Mutations Produced by a Single Cis-Syn Thymine-Thymine Cyclobutane Dimer in a Single-Stranded Vector

We have constructed a single-stranded vector that contains a uniquely located cis-syn T-T cyclobutane dimer by ligating a synthetic oligomer containing this UV photoproduct into M13mp7 viral DNA linearized with EcoRI. In the absence of SOS induction, transfection of a uvrA6 mutant of Escherichia col...

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Veröffentlicht in:	Proc. Natl. Acad. Sci. U.S.A.; (United States) 1988-11, Vol.85 (21), p.8141-8145
Hauptverfasser:	Banerjee, Swapan K., Christensen, Roshan B., Lawrence, Christopher W., LeClerc, J. Eugene
Format:	Artikel
Sprache:	eng
Schlagworte:	AMINO ACID SEQUENCE BACTERIA Bacteriophages Base Sequence Biological and medical sciences BIOLOGICAL EFFECTS BIOLOGICAL RADIATION EFFECTS BIOLOGICAL RECOVERY BIOLOGICAL REPAIR Blacklights CHEMISTRY Cloning, Molecular Cyclobutanes Dimers DNA DNA REPAIR DNA, Bacterial - radiation effects DNA, Single-Stranded - radiation effects ELECTROMAGNETIC RADIATION ESCHERICHIA COLI Escherichia coli - genetics Fundamental and applied biological sciences. Psychology GENETIC EFFECTS Genetic mutation GENETIC RADIATION EFFECTS Genetic SOS response Genetic vectors MICROORGANISMS Molecular and cellular biology Molecular genetics Molecular Sequence Data MOLECULAR STRUCTURE Molecules Mutagenesis. Repair Mutation MUTATION FREQUENCY MUTATIONS Nucleic Acid Conformation NUCLEIC ACIDS Nucleotides Oligomers ORGANIC COMPOUNDS PHOTOCHEMISTRY RADIATION EFFECTS RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT RADIATIONS RADIOINDUCTION RECOVERY REPAIR 560130 > Radiation Effects on Microorganisms Scaffolds Transfection ULTRAVIOLET RADIATION Ultraviolet Rays
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