Endothelial Cell-Derived Basic Fibroblast Growth Factor: Synthesis and Deposition into Subendothelial Extracellular Matrix
Bovine aortic and corneal endothelial cells synthesize a growth factor that remains mostly cell-associated but can also be extracted from the subendothelial extracellular matrix (ECM) deposited by these cells. The endothelial cell-derived growth factors extracted from cell lysates and from the extra...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1987-04, Vol.84 (8), p.2292-2296 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Vlodavsky, Israel Folkman, Judah Sullivan, Robert Fridman, Rafael Ishai-Michaeli, Rivka Sasse, Joachim Klagsbrun, Michael |
| description | Bovine aortic and corneal endothelial cells synthesize a growth factor that remains mostly cell-associated but can also be extracted from the subendothelial extracellular matrix (ECM) deposited by these cells. The endothelial cell-derived growth factors extracted from cell lysates and from the extracellular matrix appear to be structurally related to basic fibroblast growth factor by the criteria that they (i) bind to heparin-Sepharose and are eluted at 1.4-1.6 M NaCl, (ii) have a molecular weight of about 18,400, (iii) cross-react with anti-basic fibroblast growth factor antibodies when analyzed by electrophoretic blotting and immunoprecipitation, and (iv) are potent mitogens for bovine aortic and capillary endothelial cells. It is suggested that endothelium can store growth factors capable of autocrine growth promotion in two ways: by sequestering growth factor within the cell and by incorporating it into the underlying extracellular matrix. |
| doi_str_mv | 10.1073/pnas.84.8.2292 |
| format | Article |
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The endothelial cell-derived growth factors extracted from cell lysates and from the extracellular matrix appear to be structurally related to basic fibroblast growth factor by the criteria that they (i) bind to heparin-Sepharose and are eluted at 1.4-1.6 M NaCl, (ii) have a molecular weight of about 18,400, (iii) cross-react with anti-basic fibroblast growth factor antibodies when analyzed by electrophoretic blotting and immunoprecipitation, and (iv) are potent mitogens for bovine aortic and capillary endothelial cells. It is suggested that endothelium can store growth factors capable of autocrine growth promotion in two ways: by sequestering growth factor within the cell and by incorporating it into the underlying extracellular matrix.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.84.8.2292</identifier><identifier>PMID: 3470794</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>3T3 cells ; Animals ; Antiserum ; Aorta - metabolism ; Biological and medical sciences ; Cattle ; Cell differentiation, maturation, development, hematopoiesis ; Cell growth ; Cell physiology ; Cells ; Cells, Cultured ; Chromatography ; Clone Cells ; Cornea - metabolism ; Cultured cells ; Endothelial cells ; Endothelial growth factors ; Endothelium - cytology ; Endothelium - metabolism ; Extracellular matrix ; Extracellular Matrix - metabolism ; Fibroblast Growth Factors - biosynthesis ; Fibroblast Growth Factors - isolation & purification ; Fibroblast Growth Factors - metabolism ; Fundamental and applied biological sciences. Psychology ; Kinetics ; Molecular and cellular biology ; Ungulates</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1987-04, Vol.84 (8), p.2292-2296</ispartof><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c516t-41d7188f083f95f0822dea25ed84e909422bb4e6a0b1108a77ca1994da0294ee3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/84/8.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/29698$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/29698$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8210483$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3470794$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vlodavsky, Israel</creatorcontrib><creatorcontrib>Folkman, Judah</creatorcontrib><creatorcontrib>Sullivan, Robert</creatorcontrib><creatorcontrib>Fridman, Rafael</creatorcontrib><creatorcontrib>Ishai-Michaeli, Rivka</creatorcontrib><creatorcontrib>Sasse, Joachim</creatorcontrib><creatorcontrib>Klagsbrun, Michael</creatorcontrib><title>Endothelial Cell-Derived Basic Fibroblast Growth Factor: Synthesis and Deposition into Subendothelial Extracellular Matrix</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Bovine aortic and corneal endothelial cells synthesize a growth factor that remains mostly cell-associated but can also be extracted from the subendothelial extracellular matrix (ECM) deposited by these cells. The endothelial cell-derived growth factors extracted from cell lysates and from the extracellular matrix appear to be structurally related to basic fibroblast growth factor by the criteria that they (i) bind to heparin-Sepharose and are eluted at 1.4-1.6 M NaCl, (ii) have a molecular weight of about 18,400, (iii) cross-react with anti-basic fibroblast growth factor antibodies when analyzed by electrophoretic blotting and immunoprecipitation, and (iv) are potent mitogens for bovine aortic and capillary endothelial cells. It is suggested that endothelium can store growth factors capable of autocrine growth promotion in two ways: by sequestering growth factor within the cell and by incorporating it into the underlying extracellular matrix.</description><subject>3T3 cells</subject><subject>Animals</subject><subject>Antiserum</subject><subject>Aorta - metabolism</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Cell differentiation, maturation, development, hematopoiesis</subject><subject>Cell growth</subject><subject>Cell physiology</subject><subject>Cells</subject><subject>Cells, Cultured</subject><subject>Chromatography</subject><subject>Clone Cells</subject><subject>Cornea - metabolism</subject><subject>Cultured cells</subject><subject>Endothelial cells</subject><subject>Endothelial growth factors</subject><subject>Endothelium - cytology</subject><subject>Endothelium - metabolism</subject><subject>Extracellular matrix</subject><subject>Extracellular Matrix - metabolism</subject><subject>Fibroblast Growth Factors - biosynthesis</subject><subject>Fibroblast Growth Factors - isolation & purification</subject><subject>Fibroblast Growth Factors - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Kinetics</subject><subject>Molecular and cellular biology</subject><subject>Ungulates</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUs9v0zAUjhBodIMrByQkH9BuKc-Om9hIHKBrB9IQh8HZekkc6smNi-2Mjr8eRy1RkZA4vcP3433Pn7PsBYU5hap4s-sxzAWfizljkj3KZhQkzUsu4XE2A2BVLjjjT7PzEO4AQC4EnGVnBa-gknyW_Vr1rYsbbQ1astTW5lfam3vdkg8YTEPWpvauthgiufbuZ9yQNTbR-bfk9qFPumACwb4lV3rngonG9cT00ZHbodYnzqt99Ngk-8GiJ58xerN_lj3p0Ab9_Dgvsm_r1dflx_zmy_Wn5fubvFnQMuacthUVogNRdHKRBmOtRrbQreBaguSM1TXXJUJNKQisqgaplLxFYJJrXVxk7w6-u6He6rbRfcpi1c6bLfoH5dCov5HebNR3d68K4GVRJv3lUe_dj0GHqLYmjLdgr90QVFXxklJa_ZdI06MXAKPj_EBsvAvB624KQ0GNraqxVSW4EmpsNQlenZ4w0Y81Jvz1EcfQoO089o0JE00wClwUJwFH-wn9s0Z1g7VR7-PJvn8SE_7ygN-F9BcmApOlFMVvqs3N6w</recordid><startdate>19870401</startdate><enddate>19870401</enddate><creator>Vlodavsky, Israel</creator><creator>Folkman, Judah</creator><creator>Sullivan, Robert</creator><creator>Fridman, Rafael</creator><creator>Ishai-Michaeli, Rivka</creator><creator>Sasse, Joachim</creator><creator>Klagsbrun, Michael</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19870401</creationdate><title>Endothelial Cell-Derived Basic Fibroblast Growth Factor: Synthesis and Deposition into Subendothelial Extracellular Matrix</title><author>Vlodavsky, Israel ; Folkman, Judah ; Sullivan, Robert ; Fridman, Rafael ; Ishai-Michaeli, Rivka ; Sasse, Joachim ; Klagsbrun, Michael</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c516t-41d7188f083f95f0822dea25ed84e909422bb4e6a0b1108a77ca1994da0294ee3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>3T3 cells</topic><topic>Animals</topic><topic>Antiserum</topic><topic>Aorta - metabolism</topic><topic>Biological and medical sciences</topic><topic>Cattle</topic><topic>Cell differentiation, maturation, development, hematopoiesis</topic><topic>Cell growth</topic><topic>Cell physiology</topic><topic>Cells</topic><topic>Cells, Cultured</topic><topic>Chromatography</topic><topic>Clone Cells</topic><topic>Cornea - metabolism</topic><topic>Cultured cells</topic><topic>Endothelial cells</topic><topic>Endothelial growth factors</topic><topic>Endothelium - cytology</topic><topic>Endothelium - metabolism</topic><topic>Extracellular matrix</topic><topic>Extracellular Matrix - metabolism</topic><topic>Fibroblast Growth Factors - biosynthesis</topic><topic>Fibroblast Growth Factors - isolation & purification</topic><topic>Fibroblast Growth Factors - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Kinetics</topic><topic>Molecular and cellular biology</topic><topic>Ungulates</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vlodavsky, Israel</creatorcontrib><creatorcontrib>Folkman, Judah</creatorcontrib><creatorcontrib>Sullivan, Robert</creatorcontrib><creatorcontrib>Fridman, Rafael</creatorcontrib><creatorcontrib>Ishai-Michaeli, Rivka</creatorcontrib><creatorcontrib>Sasse, Joachim</creatorcontrib><creatorcontrib>Klagsbrun, Michael</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vlodavsky, Israel</au><au>Folkman, Judah</au><au>Sullivan, Robert</au><au>Fridman, Rafael</au><au>Ishai-Michaeli, Rivka</au><au>Sasse, Joachim</au><au>Klagsbrun, Michael</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Endothelial Cell-Derived Basic Fibroblast Growth Factor: Synthesis and Deposition into Subendothelial Extracellular Matrix</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1987-04-01</date><risdate>1987</risdate><volume>84</volume><issue>8</issue><spage>2292</spage><epage>2296</epage><pages>2292-2296</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>Bovine aortic and corneal endothelial cells synthesize a growth factor that remains mostly cell-associated but can also be extracted from the subendothelial extracellular matrix (ECM) deposited by these cells. The endothelial cell-derived growth factors extracted from cell lysates and from the extracellular matrix appear to be structurally related to basic fibroblast growth factor by the criteria that they (i) bind to heparin-Sepharose and are eluted at 1.4-1.6 M NaCl, (ii) have a molecular weight of about 18,400, (iii) cross-react with anti-basic fibroblast growth factor antibodies when analyzed by electrophoretic blotting and immunoprecipitation, and (iv) are potent mitogens for bovine aortic and capillary endothelial cells. It is suggested that endothelium can store growth factors capable of autocrine growth promotion in two ways: by sequestering growth factor within the cell and by incorporating it into the underlying extracellular matrix.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>3470794</pmid><doi>10.1073/pnas.84.8.2292</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 3T3 cells Animals Antiserum Aorta - metabolism Biological and medical sciences Cattle Cell differentiation, maturation, development, hematopoiesis Cell growth Cell physiology Cells Cells, Cultured Chromatography Clone Cells Cornea - metabolism Cultured cells Endothelial cells Endothelial growth factors Endothelium - cytology Endothelium - metabolism Extracellular matrix Extracellular Matrix - metabolism Fibroblast Growth Factors - biosynthesis Fibroblast Growth Factors - isolation & purification Fibroblast Growth Factors - metabolism Fundamental and applied biological sciences. Psychology Kinetics Molecular and cellular biology Ungulates |
| title | Endothelial Cell-Derived Basic Fibroblast Growth Factor: Synthesis and Deposition into Subendothelial Extracellular Matrix |
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