Replication of Simian Virus 40 Origin-Containing DNA in vitro with Purified Proteins

Simian virus 40 (SV40) DNA replication dependent on the SV40 origin of replication and the SV40 large tumor (T) antigen has been reconstituted in vitro with purified protein components isolated from HeLa cells. In addition to SV40 T antigen, these components included the DNA polymerase α -primase co...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1987-04, Vol.84 (7), p.1834-1838
Hauptverfasser:	Wobbe, C. Richard, Weissbach, Lawrence, Borowiec, James A., Dean, Frank B., Murakami, Yasufumi, Bullock, Peter, Hurwitz, Jerard
Format:	Artikel
Sprache:	eng
Schlagworte:	Antigens, Polyomavirus Transforming Antigens, Viral, Tumor - metabolism Biological and medical sciences Circles Cytosol - metabolism DNA DNA Polymerase II - metabolism DNA Primase DNA Replication DNA, Viral - genetics DNA, Viral - metabolism Enzymes Fundamental and applied biological sciences. Psychology Gels HeLa cells HeLa Cells - enzymology Humans Kinetics Molecular and cellular biology Molecular genetics Oncogene Proteins, Viral - metabolism Polyomavirus Protein Kinases - metabolism Quaternary ammonium compounds Replication RNA Nucleotidyltransferases - metabolism Simian virus 40 Simian virus 40 - genetics Viral tumor antigens
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