A β -galactosidase Hybrid Protein Targeted to Nuclei as a Marker for Developmental Studies

The Escherichia coli lacZ gene has been used as an indicator gene for the study of cell lineage in vivo. To adapt this marker for gene expression studies, a sequence encoding a modified β -galactosidase and including the simian virus 40 large tumor nuclear location signal (nls-β -Gal) has been intro...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1987-10, Vol.84 (19), p.6795-6799
Hauptverfasser:	Bonnerot, Claire, Rocancourt, Didier, Briand, Pascale, Grimber, Gisèle, Nicolas, Jean-François
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals beta-Galactosidase - genetics Biological and medical sciences Biotechnology Cell Line Cell lines Cell nucleus Cell Nucleus - ultrastructure Cells, Cultured Cellular differentiation Cloning, Molecular Development Biology Embryonic stem cells Embryos Escherichia coli Escherichia coli - enzymology Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Galactosidases - genetics Gene expression Genes Genes, Bacterial Genetic Vectors Life Sciences Mice Molecular and cellular biology Molecular genetics Multipotent stem cells Nuclear membrane Plasmids Protein Kinases - genetics Recombinant Fusion Proteins - analysis Stem cells Teratoma Zygote - cytology Zygote - physiology Zygotes
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