Purification and Biochemical Characterization of Human Pluripotent Hematopoietic Colony-Stimulating Factor
Pluripotent hematopoietic colony-stimulating factor (pluripotent CSF), a protein that is constitutively produced by the human bladder carcinoma cell line 5637, has been purified from low serum (0.2% fetal calf serum)-containing conditioned medium. The purification involved sequential ammonium sulfat...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1985-03, Vol.82 (5), p.1526-1530 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Welte, Karl Platzer, Erich Lu, Li Gabrilove, Janice L. Levi, Ester Mertelsmann, Roland Malcolm A. S. Moore |
| description | Pluripotent hematopoietic colony-stimulating factor (pluripotent CSF), a protein that is constitutively produced by the human bladder carcinoma cell line 5637, has been purified from low serum (0.2% fetal calf serum)-containing conditioned medium. The purification involved sequential ammonium sulfate precipitation, ion-exchange chromatography, gel filtration, and reversed-phase high-performance liquid chromatography. The purified protein has a molecular weight of 18,000 in NaDodSO4/polyacrylamide gel electrophoresis, both by the silver staining technique and by elution of biological activity from a corresponding gel slice, and has an isoelectric point of 5.5. Pluripotent CSF supports the growth of human mixed colonies, granulocyte-macrophage colonies, and early erythroid colonies and induces differentiation of the human promyelocytic leukemic cell line HL-60 and the murine myelomonocytic leukemic cell line WEHI-3B (D+). The specific activity of the purified pluripotent CSF in the granulocyte-macrophage colony assay is 1.5× 108units/mg of protein. |
| doi_str_mv | 10.1073/pnas.82.5.1526 |
| format | Article |
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S. Moore</creator><creatorcontrib>Welte, Karl ; Platzer, Erich ; Lu, Li ; Gabrilove, Janice L. ; Levi, Ester ; Mertelsmann, Roland ; Malcolm A. S. Moore</creatorcontrib><description>Pluripotent hematopoietic colony-stimulating factor (pluripotent CSF), a protein that is constitutively produced by the human bladder carcinoma cell line 5637, has been purified from low serum (0.2% fetal calf serum)-containing conditioned medium. The purification involved sequential ammonium sulfate precipitation, ion-exchange chromatography, gel filtration, and reversed-phase high-performance liquid chromatography. The purified protein has a molecular weight of 18,000 in NaDodSO4/polyacrylamide gel electrophoresis, both by the silver staining technique and by elution of biological activity from a corresponding gel slice, and has an isoelectric point of 5.5. Pluripotent CSF supports the growth of human mixed colonies, granulocyte-macrophage colonies, and early erythroid colonies and induces differentiation of the human promyelocytic leukemic cell line HL-60 and the murine myelomonocytic leukemic cell line WEHI-3B (D+). The specific activity of the purified pluripotent CSF in the granulocyte-macrophage colony assay is 1.5× 108units/mg of protein.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.82.5.1526</identifier><identifier>PMID: 3871951</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Biological and medical sciences ; Biological Assay ; Carcinoma ; Cell differentiation, maturation, development, hematopoiesis ; Cell Line ; Cell lines ; Cell physiology ; Chromatography ; colony-stimulating factors ; Colony-Stimulating Factors - isolation & purification ; Culture Media ; Cultured cells ; Elution ; Filtration ; Fundamental and applied biological sciences. Psychology ; Gels ; Granulocyte macrophage progenitor cells ; Hematopoietic Stem Cells - physiology ; Humans ; Isoelectric Point ; Molecular and cellular biology ; Molecular Weight ; Silver staining ; Stem cells ; tumor cell lines ; Urinary Bladder Neoplasms - analysis</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1985-03, Vol.82 (5), p.1526-1530</ispartof><rights>1985 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c582t-77a0a5f6576d154c32c55d835dda0b4468d4eccacc7c7c6a4768132ccc6d9c5c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/82/5.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/25444$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/25444$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27923,27924,53790,53792,58016,58249</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9082464$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3871951$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Welte, Karl</creatorcontrib><creatorcontrib>Platzer, Erich</creatorcontrib><creatorcontrib>Lu, Li</creatorcontrib><creatorcontrib>Gabrilove, Janice L.</creatorcontrib><creatorcontrib>Levi, Ester</creatorcontrib><creatorcontrib>Mertelsmann, Roland</creatorcontrib><creatorcontrib>Malcolm A. S. Moore</creatorcontrib><title>Purification and Biochemical Characterization of Human Pluripotent Hematopoietic Colony-Stimulating Factor</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Pluripotent hematopoietic colony-stimulating factor (pluripotent CSF), a protein that is constitutively produced by the human bladder carcinoma cell line 5637, has been purified from low serum (0.2% fetal calf serum)-containing conditioned medium. The purification involved sequential ammonium sulfate precipitation, ion-exchange chromatography, gel filtration, and reversed-phase high-performance liquid chromatography. The purified protein has a molecular weight of 18,000 in NaDodSO4/polyacrylamide gel electrophoresis, both by the silver staining technique and by elution of biological activity from a corresponding gel slice, and has an isoelectric point of 5.5. Pluripotent CSF supports the growth of human mixed colonies, granulocyte-macrophage colonies, and early erythroid colonies and induces differentiation of the human promyelocytic leukemic cell line HL-60 and the murine myelomonocytic leukemic cell line WEHI-3B (D+). The specific activity of the purified pluripotent CSF in the granulocyte-macrophage colony assay is 1.5× 108units/mg of protein.</description><subject>Biological and medical sciences</subject><subject>Biological Assay</subject><subject>Carcinoma</subject><subject>Cell differentiation, maturation, development, hematopoiesis</subject><subject>Cell Line</subject><subject>Cell lines</subject><subject>Cell physiology</subject><subject>Chromatography</subject><subject>colony-stimulating factors</subject><subject>Colony-Stimulating Factors - isolation & purification</subject><subject>Culture Media</subject><subject>Cultured cells</subject><subject>Elution</subject><subject>Filtration</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gels</subject><subject>Granulocyte macrophage progenitor cells</subject><subject>Hematopoietic Stem Cells - physiology</subject><subject>Humans</subject><subject>Isoelectric Point</subject><subject>Molecular and cellular biology</subject><subject>Molecular Weight</subject><subject>Silver staining</subject><subject>Stem cells</subject><subject>tumor cell lines</subject><subject>Urinary Bladder Neoplasms - analysis</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1rFDEYxoNY6lq9ehCEOYi3GZNMvubgQRfrFgoW1HNIM5lulkwyJhmx_vVm2XXYglByCOT5Pe9HHgBeIdggyNv3k1epEbihDaKYPQErBDtUM9LBp2AFIea1IJg8A89T2kEIOyrgOThvBUcdRSuwu5mjHaxW2QZfKd9Xn2zQWzOWJ1ettyoqnU20fw5AGKrNPCpf3bjim0I2PlcbM6ocpmBNtrpaBxf8ff0t23F2xeXvqstSI8QX4GxQLpmXx_sC_Lj8_H29qa-_frlaf7yuNRU415wrqOjAKGc9okS3WFPai5b2vYK3hDDRE6O10pqXwxThTKACac36TlPdXoAPh7rTfDuaXpcRo3JyinZU8V4GZeVDxdutvAu_ZNtx3LHif3f0x_BzNinL0SZtnFPehDlJzso3dgI-CiKCKBRUFLA5gDqGlKIZlmEQlPsU5T5FKbCkcp9iMbw5XWHBj7EV_e1RV6nkNETltU0L1kGBCSMnA-7L_1OXNnKYncvmdz7p91-w6K8P-i6VIBcAU0JI-xcYWcmM</recordid><startdate>19850301</startdate><enddate>19850301</enddate><creator>Welte, Karl</creator><creator>Platzer, Erich</creator><creator>Lu, Li</creator><creator>Gabrilove, Janice L.</creator><creator>Levi, Ester</creator><creator>Mertelsmann, Roland</creator><creator>Malcolm A. S. Moore</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>C1K</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19850301</creationdate><title>Purification and Biochemical Characterization of Human Pluripotent Hematopoietic Colony-Stimulating Factor</title><author>Welte, Karl ; Platzer, Erich ; Lu, Li ; Gabrilove, Janice L. ; Levi, Ester ; Mertelsmann, Roland ; Malcolm A. S. Moore</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c582t-77a0a5f6576d154c32c55d835dda0b4468d4eccacc7c7c6a4768132ccc6d9c5c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Biological and medical sciences</topic><topic>Biological Assay</topic><topic>Carcinoma</topic><topic>Cell differentiation, maturation, development, hematopoiesis</topic><topic>Cell Line</topic><topic>Cell lines</topic><topic>Cell physiology</topic><topic>Chromatography</topic><topic>colony-stimulating factors</topic><topic>Colony-Stimulating Factors - isolation & purification</topic><topic>Culture Media</topic><topic>Cultured cells</topic><topic>Elution</topic><topic>Filtration</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gels</topic><topic>Granulocyte macrophage progenitor cells</topic><topic>Hematopoietic Stem Cells - physiology</topic><topic>Humans</topic><topic>Isoelectric Point</topic><topic>Molecular and cellular biology</topic><topic>Molecular Weight</topic><topic>Silver staining</topic><topic>Stem cells</topic><topic>tumor cell lines</topic><topic>Urinary Bladder Neoplasms - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Welte, Karl</creatorcontrib><creatorcontrib>Platzer, Erich</creatorcontrib><creatorcontrib>Lu, Li</creatorcontrib><creatorcontrib>Gabrilove, Janice L.</creatorcontrib><creatorcontrib>Levi, Ester</creatorcontrib><creatorcontrib>Mertelsmann, Roland</creatorcontrib><creatorcontrib>Malcolm A. S. 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Moore</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Purification and Biochemical Characterization of Human Pluripotent Hematopoietic Colony-Stimulating Factor</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1985-03-01</date><risdate>1985</risdate><volume>82</volume><issue>5</issue><spage>1526</spage><epage>1530</epage><pages>1526-1530</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>Pluripotent hematopoietic colony-stimulating factor (pluripotent CSF), a protein that is constitutively produced by the human bladder carcinoma cell line 5637, has been purified from low serum (0.2% fetal calf serum)-containing conditioned medium. The purification involved sequential ammonium sulfate precipitation, ion-exchange chromatography, gel filtration, and reversed-phase high-performance liquid chromatography. The purified protein has a molecular weight of 18,000 in NaDodSO4/polyacrylamide gel electrophoresis, both by the silver staining technique and by elution of biological activity from a corresponding gel slice, and has an isoelectric point of 5.5. Pluripotent CSF supports the growth of human mixed colonies, granulocyte-macrophage colonies, and early erythroid colonies and induces differentiation of the human promyelocytic leukemic cell line HL-60 and the murine myelomonocytic leukemic cell line WEHI-3B (D+). The specific activity of the purified pluripotent CSF in the granulocyte-macrophage colony assay is 1.5× 108units/mg of protein.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>3871951</pmid><doi>10.1073/pnas.82.5.1526</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1985-03, Vol.82 (5), p.1526-1530 |
| issn | 0027-8424 1091-6490 |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Biological and medical sciences Biological Assay Carcinoma Cell differentiation, maturation, development, hematopoiesis Cell Line Cell lines Cell physiology Chromatography colony-stimulating factors Colony-Stimulating Factors - isolation & purification Culture Media Cultured cells Elution Filtration Fundamental and applied biological sciences. Psychology Gels Granulocyte macrophage progenitor cells Hematopoietic Stem Cells - physiology Humans Isoelectric Point Molecular and cellular biology Molecular Weight Silver staining Stem cells tumor cell lines Urinary Bladder Neoplasms - analysis |
| title | Purification and Biochemical Characterization of Human Pluripotent Hematopoietic Colony-Stimulating Factor |
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