Isolation and Amino Acid Sequence of Corticotropin-Releasing Factor from Pig Hypothalami

A polypeptide was isolated from acid extracts of porcine hypothalami on the basis of its high ability to stimulate the release of corticotropin from superfused rat pituitary cells. After an initial separation by gel filtration on Sephadex G-25, further purification was carried out by reversed-phase...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1985-12, Vol.82 (24), p.8762-8766
Hauptverfasser:	Patthy, Miklos, Horvath, Judit, Mason-Garcia, Meredith, Szoke, Balazs, Schlesinger, David H., Schally, Andrew V.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Amino acids Aminoacids, peptides. Hormones. Neuropeptides Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Chromatography, High Pressure Liquid Corticotropin-Releasing Hormone - isolation & purification Elution Endocrinology Fundamental and applied biological sciences. Psychology Hormones Hypothalamus - analysis Medical sciences Proteins Rats Sequencing Sheep Solvents Swine
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