Interaction of Endothelial Cell Growth Factor with Heparin: Characterization by Receptor and Antibody Recognition
Endothelial cell growth factor (ECGF) binds specifically in vitro to membrane receptors present on the surface of several cell types, including murine and human endothelial cells and fibroblasts. Monoclonal antibodies prepared against ECGF that inhibit the mitogenic activity of the growth factor pre...
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| Veröffentlicht in: | Proc. Natl. Acad. Sci. U.S.A.; (United States) 1985-09, Vol.82 (18), p.6138-6142 |
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| container_title | Proc. Natl. Acad. Sci. U.S.A.; (United States) |
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| creator | Schreiber, Alain B. Kenney, John Kowalski, William J. Friesel, Robert Mehlman, Tevie Maciag, Thomas |
| description | Endothelial cell growth factor (ECGF) binds specifically in vitro to membrane receptors present on the surface of several cell types, including murine and human endothelial cells and fibroblasts. Monoclonal antibodies prepared against ECGF that inhibit the mitogenic activity of the growth factor prevent receptor occupancy by the ligand. Heparin interacts structurally with ECGF [Maciag, T., Mehlman, T., Friesel, R. & Schreiber, A. B. (1984) Science 225, 932-935], potentiates the mitogenic activity of the polypeptide, restores the biological activity to inactivate ECGF, enhances the affinity of the ligand to cell surface receptors, and modifies antibody recognition of ECGF. These data suggest that the association between heparin and ECGF induces a conformational change in the polypeptide that increases or stabilizes the biological activity of the mitogen. |
| doi_str_mv | 10.1073/pnas.82.18.6138 |
| format | Article |
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Monoclonal antibodies prepared against ECGF that inhibit the mitogenic activity of the growth factor prevent receptor occupancy by the ligand. Heparin interacts structurally with ECGF [Maciag, T., Mehlman, T., Friesel, R. & Schreiber, A. B. (1984) Science 225, 932-935], potentiates the mitogenic activity of the polypeptide, restores the biological activity to inactivate ECGF, enhances the affinity of the ligand to cell surface receptors, and modifies antibody recognition of ECGF. These data suggest that the association between heparin and ECGF induces a conformational change in the polypeptide that increases or stabilizes the biological activity of the mitogen.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.82.18.6138</identifier><identifier>PMID: 2412230</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>550601 - Medicine- Unsealed Radionuclides in Diagnostics ; AMINES ; ANIMAL CELLS ; ANIMAL TISSUES ; ANIMALS ; ANTIBODIES ; ANTICOAGULANTS ; BETA DECAY RADIOISOTOPES ; Biological and medical sciences ; BODY ; CARBOHYDRATES ; CELL CONSTITUENTS ; Cell Division - drug effects ; Cell growth ; CELL MEMBRANES ; Cell physiology ; Cells ; Cells, Cultured ; CONFIGURATION INTERACTION ; CONFORMATIONAL CHANGES ; CONNECTIVE TISSUE CELLS ; DAYS LIVING RADIOISOTOPES ; DRUGS ; ELECTRON CAPTURE RADIOISOTOPES ; Endothelial cells ; Endothelial Growth Factors ; ENDOTHELIUM ; Endothelium - metabolism ; Epitopes ; FIBROBLASTS ; Fibroblasts - metabolism ; Fundamental and applied biological sciences. Psychology ; Growth Substances - immunology ; Growth Substances - metabolism ; HEMATOLOGIC AGENTS ; HEPARIN ; Heparin - pharmacology ; Human umbilical vein endothelial cells ; Humans ; INTERMEDIATE MASS NUCLEI ; IODINE 125 ; IODINE ISOTOPES ; ISOTOPE APPLICATIONS ; ISOTOPES ; MAMMALS ; MAN ; MEMBRANES ; MICE ; Mitosis ; Molecular and cellular biology ; MONOCLONAL ANTIBODIES ; MUCOPOLYSACCHARIDES ; NUCLEI ; ODD-EVEN NUCLEI ; ORGANIC COMPOUNDS ; ORGANIC SULFUR COMPOUNDS ; PEPTIDES ; POLYPEPTIDES ; POLYSACCHARIDES ; PRIMATES ; Protein Binding - drug effects ; Protein Conformation - drug effects ; PROTEINS ; RADIOISOTOPES ; RADIOLOGY AND NUCLEAR MEDICINE ; RADIONUCLIDE KINETICS ; RADIORECEPTOR ASSAY ; Receptors ; Receptors, Mitogen - metabolism ; Receptors, Vascular Endothelial Growth Factor ; Responses to growth factors, tumor promotors, other factors ; RODENTS ; SACCHARIDES ; SOMATIC CELLS ; TISSUES ; TRACER TECHNIQUES ; Ungulates ; VERTEBRATES</subject><ispartof>Proc. Natl. Acad. Sci. U.S.A.; (United States), 1985-09, Vol.82 (18), p.6138-6142</ispartof><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c517t-7a747581476aa9157d2491a697df5295ec3db23f8ef2adbc0cb794b1f6b8d7d03</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/82/18.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/25955$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/25955$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27903,27904,53769,53771,57995,58228</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8493711$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2412230$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/6300448$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Schreiber, Alain B.</creatorcontrib><creatorcontrib>Kenney, John</creatorcontrib><creatorcontrib>Kowalski, William J.</creatorcontrib><creatorcontrib>Friesel, Robert</creatorcontrib><creatorcontrib>Mehlman, Tevie</creatorcontrib><creatorcontrib>Maciag, Thomas</creatorcontrib><creatorcontrib>Institute of Biological Sciences, Palo Alto, CA</creatorcontrib><title>Interaction of Endothelial Cell Growth Factor with Heparin: Characterization by Receptor and Antibody Recognition</title><title>Proc. Natl. Acad. Sci. U.S.A.; (United States)</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Endothelial cell growth factor (ECGF) binds specifically in vitro to membrane receptors present on the surface of several cell types, including murine and human endothelial cells and fibroblasts. Monoclonal antibodies prepared against ECGF that inhibit the mitogenic activity of the growth factor prevent receptor occupancy by the ligand. Heparin interacts structurally with ECGF [Maciag, T., Mehlman, T., Friesel, R. & Schreiber, A. B. (1984) Science 225, 932-935], potentiates the mitogenic activity of the polypeptide, restores the biological activity to inactivate ECGF, enhances the affinity of the ligand to cell surface receptors, and modifies antibody recognition of ECGF. These data suggest that the association between heparin and ECGF induces a conformational change in the polypeptide that increases or stabilizes the biological activity of the mitogen.</description><subject>550601 - Medicine- Unsealed Radionuclides in Diagnostics</subject><subject>AMINES</subject><subject>ANIMAL CELLS</subject><subject>ANIMAL TISSUES</subject><subject>ANIMALS</subject><subject>ANTIBODIES</subject><subject>ANTICOAGULANTS</subject><subject>BETA DECAY RADIOISOTOPES</subject><subject>Biological and medical sciences</subject><subject>BODY</subject><subject>CARBOHYDRATES</subject><subject>CELL CONSTITUENTS</subject><subject>Cell Division - drug effects</subject><subject>Cell growth</subject><subject>CELL MEMBRANES</subject><subject>Cell physiology</subject><subject>Cells</subject><subject>Cells, Cultured</subject><subject>CONFIGURATION INTERACTION</subject><subject>CONFORMATIONAL CHANGES</subject><subject>CONNECTIVE TISSUE CELLS</subject><subject>DAYS LIVING RADIOISOTOPES</subject><subject>DRUGS</subject><subject>ELECTRON CAPTURE RADIOISOTOPES</subject><subject>Endothelial cells</subject><subject>Endothelial Growth Factors</subject><subject>ENDOTHELIUM</subject><subject>Endothelium - metabolism</subject><subject>Epitopes</subject><subject>FIBROBLASTS</subject><subject>Fibroblasts - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Growth Substances - immunology</subject><subject>Growth Substances - metabolism</subject><subject>HEMATOLOGIC AGENTS</subject><subject>HEPARIN</subject><subject>Heparin - pharmacology</subject><subject>Human umbilical vein endothelial cells</subject><subject>Humans</subject><subject>INTERMEDIATE MASS NUCLEI</subject><subject>IODINE 125</subject><subject>IODINE ISOTOPES</subject><subject>ISOTOPE APPLICATIONS</subject><subject>ISOTOPES</subject><subject>MAMMALS</subject><subject>MAN</subject><subject>MEMBRANES</subject><subject>MICE</subject><subject>Mitosis</subject><subject>Molecular and cellular biology</subject><subject>MONOCLONAL ANTIBODIES</subject><subject>MUCOPOLYSACCHARIDES</subject><subject>NUCLEI</subject><subject>ODD-EVEN NUCLEI</subject><subject>ORGANIC COMPOUNDS</subject><subject>ORGANIC SULFUR COMPOUNDS</subject><subject>PEPTIDES</subject><subject>POLYPEPTIDES</subject><subject>POLYSACCHARIDES</subject><subject>PRIMATES</subject><subject>Protein Binding - drug effects</subject><subject>Protein Conformation - drug effects</subject><subject>PROTEINS</subject><subject>RADIOISOTOPES</subject><subject>RADIOLOGY AND NUCLEAR MEDICINE</subject><subject>RADIONUCLIDE KINETICS</subject><subject>RADIORECEPTOR ASSAY</subject><subject>Receptors</subject><subject>Receptors, Mitogen - metabolism</subject><subject>Receptors, Vascular Endothelial Growth Factor</subject><subject>Responses to growth factors, tumor promotors, other factors</subject><subject>RODENTS</subject><subject>SACCHARIDES</subject><subject>SOMATIC CELLS</subject><subject>TISSUES</subject><subject>TRACER TECHNIQUES</subject><subject>Ungulates</subject><subject>VERTEBRATES</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kd1rFDEUxYModV19FgRlENGn2eZzkgg-lKVfUBBEn0Mmk-mkzCbbJGutf72Z7rDWF59yyfndew_3APAawRWCnBxvvU4rgVdIrBpExBOwQFCiuqESPgULCDGvBcX0OXiR0g2EUDIBj8ARpghjAhfg9tJnG7XJLvgq9NWp70Ie7Oj0WK3tOFbnMdzloTorSIjVnSv1hd3q6Pznaj3oqdVG91s_DGjvq2_W2O2Eat9VJz67NnQPv-Hauwl6CZ71ekz21fwuwY-z0-_ri_rq6_nl-uSqNgzxXHPNKWcCUd5oLRHjHaYS6UbyrmdYMmtI12LSC9tj3bUGmpZL2qK-aUXHO0iW4Mt-7nbXbmxnrM9Rj2ob3UbHexW0U_8q3g3qOvxURCJYTrsE7_f9IWWnknHZmsEE763JqiEQUioK9HFeEsPtzqasNi6ZcjftbdglxRvCKMWkgMd70MSQUrT9wQiCakpSTUkqgRUSakqydLx97P_Az9EV_cOs62T02EftjUsHTFBJOEIFezdj0_yD-njPp_8Cqt-NY7a_ciHf7MmbVAL-a4hJxsgfqDbJ7A</recordid><startdate>19850901</startdate><enddate>19850901</enddate><creator>Schreiber, Alain B.</creator><creator>Kenney, John</creator><creator>Kowalski, William J.</creator><creator>Friesel, Robert</creator><creator>Mehlman, Tevie</creator><creator>Maciag, Thomas</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope><scope>5PM</scope></search><sort><creationdate>19850901</creationdate><title>Interaction of Endothelial Cell Growth Factor with Heparin: Characterization by Receptor and Antibody Recognition</title><author>Schreiber, Alain B. ; Kenney, John ; Kowalski, William J. ; Friesel, Robert ; Mehlman, Tevie ; Maciag, Thomas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c517t-7a747581476aa9157d2491a697df5295ec3db23f8ef2adbc0cb794b1f6b8d7d03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>550601 - Medicine- Unsealed Radionuclides in Diagnostics</topic><topic>AMINES</topic><topic>ANIMAL CELLS</topic><topic>ANIMAL TISSUES</topic><topic>ANIMALS</topic><topic>ANTIBODIES</topic><topic>ANTICOAGULANTS</topic><topic>BETA DECAY RADIOISOTOPES</topic><topic>Biological and medical sciences</topic><topic>BODY</topic><topic>CARBOHYDRATES</topic><topic>CELL CONSTITUENTS</topic><topic>Cell Division - drug effects</topic><topic>Cell growth</topic><topic>CELL MEMBRANES</topic><topic>Cell physiology</topic><topic>Cells</topic><topic>Cells, Cultured</topic><topic>CONFIGURATION INTERACTION</topic><topic>CONFORMATIONAL CHANGES</topic><topic>CONNECTIVE TISSUE CELLS</topic><topic>DAYS LIVING RADIOISOTOPES</topic><topic>DRUGS</topic><topic>ELECTRON CAPTURE RADIOISOTOPES</topic><topic>Endothelial cells</topic><topic>Endothelial Growth Factors</topic><topic>ENDOTHELIUM</topic><topic>Endothelium - metabolism</topic><topic>Epitopes</topic><topic>FIBROBLASTS</topic><topic>Fibroblasts - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Growth Substances - immunology</topic><topic>Growth Substances - metabolism</topic><topic>HEMATOLOGIC AGENTS</topic><topic>HEPARIN</topic><topic>Heparin - pharmacology</topic><topic>Human umbilical vein endothelial cells</topic><topic>Humans</topic><topic>INTERMEDIATE MASS NUCLEI</topic><topic>IODINE 125</topic><topic>IODINE ISOTOPES</topic><topic>ISOTOPE APPLICATIONS</topic><topic>ISOTOPES</topic><topic>MAMMALS</topic><topic>MAN</topic><topic>MEMBRANES</topic><topic>MICE</topic><topic>Mitosis</topic><topic>Molecular and cellular biology</topic><topic>MONOCLONAL ANTIBODIES</topic><topic>MUCOPOLYSACCHARIDES</topic><topic>NUCLEI</topic><topic>ODD-EVEN NUCLEI</topic><topic>ORGANIC COMPOUNDS</topic><topic>ORGANIC SULFUR COMPOUNDS</topic><topic>PEPTIDES</topic><topic>POLYPEPTIDES</topic><topic>POLYSACCHARIDES</topic><topic>PRIMATES</topic><topic>Protein Binding - drug effects</topic><topic>Protein Conformation - drug effects</topic><topic>PROTEINS</topic><topic>RADIOISOTOPES</topic><topic>RADIOLOGY AND NUCLEAR MEDICINE</topic><topic>RADIONUCLIDE KINETICS</topic><topic>RADIORECEPTOR ASSAY</topic><topic>Receptors</topic><topic>Receptors, Mitogen - metabolism</topic><topic>Receptors, Vascular Endothelial Growth Factor</topic><topic>Responses to growth factors, tumor promotors, other factors</topic><topic>RODENTS</topic><topic>SACCHARIDES</topic><topic>SOMATIC CELLS</topic><topic>TISSUES</topic><topic>TRACER TECHNIQUES</topic><topic>Ungulates</topic><topic>VERTEBRATES</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schreiber, Alain B.</creatorcontrib><creatorcontrib>Kenney, John</creatorcontrib><creatorcontrib>Kowalski, William J.</creatorcontrib><creatorcontrib>Friesel, Robert</creatorcontrib><creatorcontrib>Mehlman, Tevie</creatorcontrib><creatorcontrib>Maciag, Thomas</creatorcontrib><creatorcontrib>Institute of Biological Sciences, Palo Alto, CA</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proc. Natl. Acad. Sci. U.S.A.; (United States)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schreiber, Alain B.</au><au>Kenney, John</au><au>Kowalski, William J.</au><au>Friesel, Robert</au><au>Mehlman, Tevie</au><au>Maciag, Thomas</au><aucorp>Institute of Biological Sciences, Palo Alto, CA</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Interaction of Endothelial Cell Growth Factor with Heparin: Characterization by Receptor and Antibody Recognition</atitle><jtitle>Proc. Natl. Acad. Sci. U.S.A.; (United States)</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1985-09-01</date><risdate>1985</risdate><volume>82</volume><issue>18</issue><spage>6138</spage><epage>6142</epage><pages>6138-6142</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>Endothelial cell growth factor (ECGF) binds specifically in vitro to membrane receptors present on the surface of several cell types, including murine and human endothelial cells and fibroblasts. Monoclonal antibodies prepared against ECGF that inhibit the mitogenic activity of the growth factor prevent receptor occupancy by the ligand. Heparin interacts structurally with ECGF [Maciag, T., Mehlman, T., Friesel, R. & Schreiber, A. B. (1984) Science 225, 932-935], potentiates the mitogenic activity of the polypeptide, restores the biological activity to inactivate ECGF, enhances the affinity of the ligand to cell surface receptors, and modifies antibody recognition of ECGF. These data suggest that the association between heparin and ECGF induces a conformational change in the polypeptide that increases or stabilizes the biological activity of the mitogen.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>2412230</pmid><doi>10.1073/pnas.82.18.6138</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0027-8424 |
| ispartof | Proc. Natl. Acad. Sci. U.S.A.; (United States), 1985-09, Vol.82 (18), p.6138-6142 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_pnas_primary_82_18_6138 |
| source | MEDLINE; Jstor Complete Legacy; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | 550601 - Medicine- Unsealed Radionuclides in Diagnostics AMINES ANIMAL CELLS ANIMAL TISSUES ANIMALS ANTIBODIES ANTICOAGULANTS BETA DECAY RADIOISOTOPES Biological and medical sciences BODY CARBOHYDRATES CELL CONSTITUENTS Cell Division - drug effects Cell growth CELL MEMBRANES Cell physiology Cells Cells, Cultured CONFIGURATION INTERACTION CONFORMATIONAL CHANGES CONNECTIVE TISSUE CELLS DAYS LIVING RADIOISOTOPES DRUGS ELECTRON CAPTURE RADIOISOTOPES Endothelial cells Endothelial Growth Factors ENDOTHELIUM Endothelium - metabolism Epitopes FIBROBLASTS Fibroblasts - metabolism Fundamental and applied biological sciences. Psychology Growth Substances - immunology Growth Substances - metabolism HEMATOLOGIC AGENTS HEPARIN Heparin - pharmacology Human umbilical vein endothelial cells Humans INTERMEDIATE MASS NUCLEI IODINE 125 IODINE ISOTOPES ISOTOPE APPLICATIONS ISOTOPES MAMMALS MAN MEMBRANES MICE Mitosis Molecular and cellular biology MONOCLONAL ANTIBODIES MUCOPOLYSACCHARIDES NUCLEI ODD-EVEN NUCLEI ORGANIC COMPOUNDS ORGANIC SULFUR COMPOUNDS PEPTIDES POLYPEPTIDES POLYSACCHARIDES PRIMATES Protein Binding - drug effects Protein Conformation - drug effects PROTEINS RADIOISOTOPES RADIOLOGY AND NUCLEAR MEDICINE RADIONUCLIDE KINETICS RADIORECEPTOR ASSAY Receptors Receptors, Mitogen - metabolism Receptors, Vascular Endothelial Growth Factor Responses to growth factors, tumor promotors, other factors RODENTS SACCHARIDES SOMATIC CELLS TISSUES TRACER TECHNIQUES Ungulates VERTEBRATES |
| title | Interaction of Endothelial Cell Growth Factor with Heparin: Characterization by Receptor and Antibody Recognition |
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