Localization of the Structural Gene for Human Apolipoprotein A-I on the Long Arm of Human Chromosome 11
Apolipoprotein A-I (apo A-I), the major apolipoprotein in human high density lipoproteins, is involved in the disease atherosclerosis. Cloned apo A-I cDNA (pA1-3) was used as a probe in chromosome mapping studies to detect the human apo A-I structural gene sequence in human-Chinese hamster cell hybr...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1984-01, Vol.81 (2), p.508-511 |
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| creator | Cheung, Peter Kao, Fa-Ten Law, Martha Liao Jones, Carol Puck, Theodore T. Chan, Lawrence |
| description | Apolipoprotein A-I (apo A-I), the major apolipoprotein in human high density lipoproteins, is involved in the disease atherosclerosis. Cloned apo A-I cDNA (pA1-3) was used as a probe in chromosome mapping studies to detect the human apo A-I structural gene sequence in human-Chinese hamster cell hybrids. Southern blot analysis of 13 hybrids localized the gene to human chromosome 11. Confirmation of the chromosomal assignment was obtained by analysis of a hybrid (J1) containing a single human chromosome, no. 11. Regional mapping was achieved by using deletion subclones of J1 that localized the human apo A-I structural gene to the region 11q13→ qter. Since the human apolipoprotein C-III (apo C-III) structural gene is closely linked to apo A-I, it can be assigned to the same region on the long arm of chromosome 11. By extension of methods previously described, it now appears possible to carry out fine-structure analysis of this and related gene regions on chromosome 11 and to study the biochemical concomitants of these genes and of genes on other chromosomes for analysis of their role in atherosclerosis. |
| doi_str_mv | 10.1073/pnas.81.2.508 |
| format | Article |
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Cloned apo A-I cDNA (pA1-3) was used as a probe in chromosome mapping studies to detect the human apo A-I structural gene sequence in human-Chinese hamster cell hybrids. Southern blot analysis of 13 hybrids localized the gene to human chromosome 11. Confirmation of the chromosomal assignment was obtained by analysis of a hybrid (J1) containing a single human chromosome, no. 11. Regional mapping was achieved by using deletion subclones of J1 that localized the human apo A-I structural gene to the region 11q13→ qter. Since the human apolipoprotein C-III (apo C-III) structural gene is closely linked to apo A-I, it can be assigned to the same region on the long arm of chromosome 11. By extension of methods previously described, it now appears possible to carry out fine-structure analysis of this and related gene regions on chromosome 11 and to study the biochemical concomitants of these genes and of genes on other chromosomes for analysis of their role in atherosclerosis.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.81.2.508</identifier><identifier>PMID: 6420790</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Animals ; Apolipoprotein A-I ; Apolipoproteins - genetics ; Atherosclerosis ; Cell lines ; Chromosome Deletion ; Chromosome Mapping ; Chromosomes ; Chromosomes, Human, 6-12 and X ; Complementary DNA ; Cricetinae ; DNA ; Genes ; HDL lipoproteins ; Human chromosomes ; Humans ; Hybrid Cells ; Hybridity ; Nucleic Acid Hybridization ; Somatic cells</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1984-01, Vol.81 (2), p.508-511</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c482t-af100a034e36bb22f3a14ef17078321c2e2a496f7b045b61e5c1d82679a6018a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/81/2.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/22713$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/22713$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6420790$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cheung, Peter</creatorcontrib><creatorcontrib>Kao, Fa-Ten</creatorcontrib><creatorcontrib>Law, Martha Liao</creatorcontrib><creatorcontrib>Jones, Carol</creatorcontrib><creatorcontrib>Puck, Theodore T.</creatorcontrib><creatorcontrib>Chan, Lawrence</creatorcontrib><title>Localization of the Structural Gene for Human Apolipoprotein A-I on the Long Arm of Human Chromosome 11</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Apolipoprotein A-I (apo A-I), the major apolipoprotein in human high density lipoproteins, is involved in the disease atherosclerosis. Cloned apo A-I cDNA (pA1-3) was used as a probe in chromosome mapping studies to detect the human apo A-I structural gene sequence in human-Chinese hamster cell hybrids. Southern blot analysis of 13 hybrids localized the gene to human chromosome 11. Confirmation of the chromosomal assignment was obtained by analysis of a hybrid (J1) containing a single human chromosome, no. 11. Regional mapping was achieved by using deletion subclones of J1 that localized the human apo A-I structural gene to the region 11q13→ qter. Since the human apolipoprotein C-III (apo C-III) structural gene is closely linked to apo A-I, it can be assigned to the same region on the long arm of chromosome 11. By extension of methods previously described, it now appears possible to carry out fine-structure analysis of this and related gene regions on chromosome 11 and to study the biochemical concomitants of these genes and of genes on other chromosomes for analysis of their role in atherosclerosis.</description><subject>Animals</subject><subject>Apolipoprotein A-I</subject><subject>Apolipoproteins - genetics</subject><subject>Atherosclerosis</subject><subject>Cell lines</subject><subject>Chromosome Deletion</subject><subject>Chromosome Mapping</subject><subject>Chromosomes</subject><subject>Chromosomes, Human, 6-12 and X</subject><subject>Complementary DNA</subject><subject>Cricetinae</subject><subject>DNA</subject><subject>Genes</subject><subject>HDL lipoproteins</subject><subject>Human chromosomes</subject><subject>Humans</subject><subject>Hybrid Cells</subject><subject>Hybridity</subject><subject>Nucleic Acid Hybridization</subject><subject>Somatic cells</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkbFv1DAUhy0Eao_CyFIVyQtsOd6zncQZOpxO0FY6iaFltpzUvkuVxKntVIW_Hkd3PcECk2V93-_ZTz9CPiAsEUr-ZRx0WEpcsmUO8hVZIFSYFaKC12QBwMpMCiZOydsQHgCgyiWckJNCMCgrWJDtxjW6a3_p2LqBOkvjztDb6KcmTl539MoMhlrn6fXU64GuRte1oxu9i6ZN1-yGptic2bhhS1e-n2fs3fXOu94F1xuK-I68sboL5v3hPCM_vn29W19nm-9XN-vVJmuEZDHTFgE0cGF4UdeMWa5RGIsllJIzbJhhWlSFLWsQeV2gyRu8l6woK10ASs3PyOV-7jjVvblvzBDTGmr0ba_9T-V0q_4mQ7tTW_ekuBDpkZT_fMh79ziZEFXfhsZ0nR6Mm4KSUAkhOP-viFwWOeMiidlebLwLwRt7_AyCmhtUc4NKomIqNZj8j39ucLQPlSX-6cDn2At9iSs7dV00zzF5F__wEj7f44cQnT9yxkrk_DdpM7b4</recordid><startdate>19840101</startdate><enddate>19840101</enddate><creator>Cheung, Peter</creator><creator>Kao, Fa-Ten</creator><creator>Law, Martha Liao</creator><creator>Jones, Carol</creator><creator>Puck, Theodore T.</creator><creator>Chan, Lawrence</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19840101</creationdate><title>Localization of the Structural Gene for Human Apolipoprotein A-I on the Long Arm of Human Chromosome 11</title><author>Cheung, Peter ; Kao, Fa-Ten ; Law, Martha Liao ; Jones, Carol ; Puck, Theodore T. ; Chan, Lawrence</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c482t-af100a034e36bb22f3a14ef17078321c2e2a496f7b045b61e5c1d82679a6018a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Animals</topic><topic>Apolipoprotein A-I</topic><topic>Apolipoproteins - genetics</topic><topic>Atherosclerosis</topic><topic>Cell lines</topic><topic>Chromosome Deletion</topic><topic>Chromosome Mapping</topic><topic>Chromosomes</topic><topic>Chromosomes, Human, 6-12 and X</topic><topic>Complementary DNA</topic><topic>Cricetinae</topic><topic>DNA</topic><topic>Genes</topic><topic>HDL lipoproteins</topic><topic>Human chromosomes</topic><topic>Humans</topic><topic>Hybrid Cells</topic><topic>Hybridity</topic><topic>Nucleic Acid Hybridization</topic><topic>Somatic cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cheung, Peter</creatorcontrib><creatorcontrib>Kao, Fa-Ten</creatorcontrib><creatorcontrib>Law, Martha Liao</creatorcontrib><creatorcontrib>Jones, Carol</creatorcontrib><creatorcontrib>Puck, Theodore T.</creatorcontrib><creatorcontrib>Chan, Lawrence</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cheung, Peter</au><au>Kao, Fa-Ten</au><au>Law, Martha Liao</au><au>Jones, Carol</au><au>Puck, Theodore T.</au><au>Chan, Lawrence</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Localization of the Structural Gene for Human Apolipoprotein A-I on the Long Arm of Human Chromosome 11</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1984-01-01</date><risdate>1984</risdate><volume>81</volume><issue>2</issue><spage>508</spage><epage>511</epage><pages>508-511</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Apolipoprotein A-I (apo A-I), the major apolipoprotein in human high density lipoproteins, is involved in the disease atherosclerosis. Cloned apo A-I cDNA (pA1-3) was used as a probe in chromosome mapping studies to detect the human apo A-I structural gene sequence in human-Chinese hamster cell hybrids. Southern blot analysis of 13 hybrids localized the gene to human chromosome 11. Confirmation of the chromosomal assignment was obtained by analysis of a hybrid (J1) containing a single human chromosome, no. 11. Regional mapping was achieved by using deletion subclones of J1 that localized the human apo A-I structural gene to the region 11q13→ qter. Since the human apolipoprotein C-III (apo C-III) structural gene is closely linked to apo A-I, it can be assigned to the same region on the long arm of chromosome 11. By extension of methods previously described, it now appears possible to carry out fine-structure analysis of this and related gene regions on chromosome 11 and to study the biochemical concomitants of these genes and of genes on other chromosomes for analysis of their role in atherosclerosis.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>6420790</pmid><doi>10.1073/pnas.81.2.508</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| source | Jstor Complete Legacy; MEDLINE; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Animals Apolipoprotein A-I Apolipoproteins - genetics Atherosclerosis Cell lines Chromosome Deletion Chromosome Mapping Chromosomes Chromosomes, Human, 6-12 and X Complementary DNA Cricetinae DNA Genes HDL lipoproteins Human chromosomes Humans Hybrid Cells Hybridity Nucleic Acid Hybridization Somatic cells |
| title | Localization of the Structural Gene for Human Apolipoprotein A-I on the Long Arm of Human Chromosome 11 |
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