Specific Regulation of c-myc Oncogene Expression in a Murine B-Cell Lymphoma
The c-myc oncogene has been implicated in a wide spectrum of B-cell neoplasias. In normal cells, the level of expression of the c-myc gene correlates with growth status. In the present study, we examined the effect of receptor-mediated inhibition of growth on c-myc expression in a B-cell lymphoma. T...
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Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1984-09, Vol.81 (17), p.5546-5550 |
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creator | McCormack, James E. Pepe, Vincent H. Kent, Rachel B. Dean, Michael Marshak-Rothstein, Ann Sonenshein, Gail E. |
description | The c-myc oncogene has been implicated in a wide spectrum of B-cell neoplasias. In normal cells, the level of expression of the c-myc gene correlates with growth status. In the present study, we examined the effect of receptor-mediated inhibition of growth on c-myc expression in a B-cell lymphoma. The murine lymphoma line WEHI 231 has been characterized as an early B cell; it bears surface-bound IgM and has unrearranged c-myc genes. Following treatment of a WEHI 231 culture with anti-mouse Ig antiserum, the cells undergo one round of division and further proliferation is inhibited. We observed that this treatment specifically affected cytoplasmic levels of c-myc mRNA. An initial early increase is followed by a precipitous drop such that by 4 hr (after exposure) the amount of c-myc mRNA is below control values by a factor of ≈ 10. The drop in c-myc precedes cessation of DNA synthesis. During the 2- to 4-hr period, c-myc mRNA had a maximal half-life of between 20 and 30 min. In contrast, even 24 hr after anti-Ig exposure, the amounts of most major mRNAs, including μ heavy chain and actin, were not significantly altered. These results indicate that expression of an unrearranged c-myc gene can be selectively responsive to receptor-mediated regulatory events. |
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In normal cells, the level of expression of the c-myc gene correlates with growth status. In the present study, we examined the effect of receptor-mediated inhibition of growth on c-myc expression in a B-cell lymphoma. The murine lymphoma line WEHI 231 has been characterized as an early B cell; it bears surface-bound IgM and has unrearranged c-myc genes. Following treatment of a WEHI 231 culture with anti-mouse Ig antiserum, the cells undergo one round of division and further proliferation is inhibited. We observed that this treatment specifically affected cytoplasmic levels of c-myc mRNA. An initial early increase is followed by a precipitous drop such that by 4 hr (after exposure) the amount of c-myc mRNA is below control values by a factor of ≈ 10. The drop in c-myc precedes cessation of DNA synthesis. During the 2- to 4-hr period, c-myc mRNA had a maximal half-life of between 20 and 30 min. In contrast, even 24 hr after anti-Ig exposure, the amounts of most major mRNAs, including μ heavy chain and actin, were not significantly altered. These results indicate that expression of an unrearranged c-myc gene can be selectively responsive to receptor-mediated regulatory events.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.81.17.5546</identifier><identifier>PMID: 6206500</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Actins ; Animals ; Antibodies ; Antigen-Antibody Complex ; Antiserum ; B lymphocytes ; B-Lymphocytes - immunology ; Cell Division ; Cell growth ; Cell Membrane - immunology ; DNA Replication ; Gene expression regulation ; Immune Sera ; Immunoglobulins - immunology ; Lymphoma - genetics ; Lymphoma - immunology ; Messenger RNA ; Mice ; Oncogenes ; Plasma cells ; Poly A - genetics ; Protein Biosynthesis ; RNA ; RNA - genetics ; RNA, Messenger - genetics ; T lymphocytes</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1984-09, Vol.81 (17), p.5546-5550</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c461t-8a5e1f783c564252fa51bf10ee53a7a64614a08de3e2ccaf04b629209487707b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/81/17.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/23944$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/23944$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6206500$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>McCormack, James E.</creatorcontrib><creatorcontrib>Pepe, Vincent H.</creatorcontrib><creatorcontrib>Kent, Rachel B.</creatorcontrib><creatorcontrib>Dean, Michael</creatorcontrib><creatorcontrib>Marshak-Rothstein, Ann</creatorcontrib><creatorcontrib>Sonenshein, Gail E.</creatorcontrib><title>Specific Regulation of c-myc Oncogene Expression in a Murine B-Cell Lymphoma</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The c-myc oncogene has been implicated in a wide spectrum of B-cell neoplasias. In normal cells, the level of expression of the c-myc gene correlates with growth status. In the present study, we examined the effect of receptor-mediated inhibition of growth on c-myc expression in a B-cell lymphoma. The murine lymphoma line WEHI 231 has been characterized as an early B cell; it bears surface-bound IgM and has unrearranged c-myc genes. Following treatment of a WEHI 231 culture with anti-mouse Ig antiserum, the cells undergo one round of division and further proliferation is inhibited. We observed that this treatment specifically affected cytoplasmic levels of c-myc mRNA. An initial early increase is followed by a precipitous drop such that by 4 hr (after exposure) the amount of c-myc mRNA is below control values by a factor of ≈ 10. The drop in c-myc precedes cessation of DNA synthesis. During the 2- to 4-hr period, c-myc mRNA had a maximal half-life of between 20 and 30 min. In contrast, even 24 hr after anti-Ig exposure, the amounts of most major mRNAs, including μ heavy chain and actin, were not significantly altered. These results indicate that expression of an unrearranged c-myc gene can be selectively responsive to receptor-mediated regulatory events.</description><subject>Actins</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Antigen-Antibody Complex</subject><subject>Antiserum</subject><subject>B lymphocytes</subject><subject>B-Lymphocytes - immunology</subject><subject>Cell Division</subject><subject>Cell growth</subject><subject>Cell Membrane - immunology</subject><subject>DNA Replication</subject><subject>Gene expression regulation</subject><subject>Immune Sera</subject><subject>Immunoglobulins - immunology</subject><subject>Lymphoma - genetics</subject><subject>Lymphoma - immunology</subject><subject>Messenger RNA</subject><subject>Mice</subject><subject>Oncogenes</subject><subject>Plasma cells</subject><subject>Poly A - genetics</subject><subject>Protein Biosynthesis</subject><subject>RNA</subject><subject>RNA - genetics</subject><subject>RNA, Messenger - genetics</subject><subject>T lymphocytes</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtv1DAURi1EVYbCGgkJlBWsMr1-xc6CBYwKrTSoEo-15XGvp66SOI0T1Pn3OJphChtWlnzOd339EfKKwpKC4ud9Z9NS0yVVSylF9YQsKNS0rEQNT8kCgKlSCyaekecp3QFALTWcktOKQSUBFmT9vUcXfHDFN9xOjR1D7IroC1e2O1dcdy5uscPi4qEfMKUZhq6wxddpCPn6U7nCpinWu7a_ja19QU68bRK-PJxn5Ofnix-ry3J9_eVq9XFdOlHRsdRWIvVKcycrwSTzVtKNp4AouVW2ypKwoG-QI3POehCbitUMaqGVArXhZ-TDfm4_bVq8cdiNg21MP4TWDjsTbTD_ki7cmm38ZXhNleA5_-6QH-L9hGk0bUgu_8R2GKdkNGVM10xl8XwvuiGmNKA_vkHBzP2buf_sG6rM3H9OvPl7taN_KDzz9wc-B__QxwHGT00z4sOYzbf_NbPwei_cpTEOR4PxWgj-G4p3olg</recordid><startdate>19840901</startdate><enddate>19840901</enddate><creator>McCormack, James E.</creator><creator>Pepe, Vincent H.</creator><creator>Kent, Rachel B.</creator><creator>Dean, Michael</creator><creator>Marshak-Rothstein, Ann</creator><creator>Sonenshein, Gail E.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19840901</creationdate><title>Specific Regulation of c-myc Oncogene Expression in a Murine B-Cell Lymphoma</title><author>McCormack, James E. ; Pepe, Vincent H. ; Kent, Rachel B. ; Dean, Michael ; Marshak-Rothstein, Ann ; Sonenshein, Gail E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c461t-8a5e1f783c564252fa51bf10ee53a7a64614a08de3e2ccaf04b629209487707b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Actins</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Antigen-Antibody Complex</topic><topic>Antiserum</topic><topic>B lymphocytes</topic><topic>B-Lymphocytes - immunology</topic><topic>Cell Division</topic><topic>Cell growth</topic><topic>Cell Membrane - immunology</topic><topic>DNA Replication</topic><topic>Gene expression regulation</topic><topic>Immune Sera</topic><topic>Immunoglobulins - immunology</topic><topic>Lymphoma - genetics</topic><topic>Lymphoma - immunology</topic><topic>Messenger RNA</topic><topic>Mice</topic><topic>Oncogenes</topic><topic>Plasma cells</topic><topic>Poly A - genetics</topic><topic>Protein Biosynthesis</topic><topic>RNA</topic><topic>RNA - genetics</topic><topic>RNA, Messenger - genetics</topic><topic>T lymphocytes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>McCormack, James E.</creatorcontrib><creatorcontrib>Pepe, Vincent H.</creatorcontrib><creatorcontrib>Kent, Rachel B.</creatorcontrib><creatorcontrib>Dean, Michael</creatorcontrib><creatorcontrib>Marshak-Rothstein, Ann</creatorcontrib><creatorcontrib>Sonenshein, Gail E.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>McCormack, James E.</au><au>Pepe, Vincent H.</au><au>Kent, Rachel B.</au><au>Dean, Michael</au><au>Marshak-Rothstein, Ann</au><au>Sonenshein, Gail E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Specific Regulation of c-myc Oncogene Expression in a Murine B-Cell Lymphoma</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1984-09-01</date><risdate>1984</risdate><volume>81</volume><issue>17</issue><spage>5546</spage><epage>5550</epage><pages>5546-5550</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>The c-myc oncogene has been implicated in a wide spectrum of B-cell neoplasias. In normal cells, the level of expression of the c-myc gene correlates with growth status. In the present study, we examined the effect of receptor-mediated inhibition of growth on c-myc expression in a B-cell lymphoma. The murine lymphoma line WEHI 231 has been characterized as an early B cell; it bears surface-bound IgM and has unrearranged c-myc genes. Following treatment of a WEHI 231 culture with anti-mouse Ig antiserum, the cells undergo one round of division and further proliferation is inhibited. We observed that this treatment specifically affected cytoplasmic levels of c-myc mRNA. An initial early increase is followed by a precipitous drop such that by 4 hr (after exposure) the amount of c-myc mRNA is below control values by a factor of ≈ 10. The drop in c-myc precedes cessation of DNA synthesis. During the 2- to 4-hr period, c-myc mRNA had a maximal half-life of between 20 and 30 min. 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subjects | Actins Animals Antibodies Antigen-Antibody Complex Antiserum B lymphocytes B-Lymphocytes - immunology Cell Division Cell growth Cell Membrane - immunology DNA Replication Gene expression regulation Immune Sera Immunoglobulins - immunology Lymphoma - genetics Lymphoma - immunology Messenger RNA Mice Oncogenes Plasma cells Poly A - genetics Protein Biosynthesis RNA RNA - genetics RNA, Messenger - genetics T lymphocytes |
title | Specific Regulation of c-myc Oncogene Expression in a Murine B-Cell Lymphoma |
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