Evidence for Use of Rare Codons in the dnaG Gene and Other Regulatory Genes of Escherichia coli
Amino acid sequence and composition data of Escherichia coli dnaG primase protein and its tryptic peptides have confirmed that the dnaG gene contains an unusually high number of codons that are not frequently used in most E. coli genes. In 25 E. coli proteins analyzed the codons AUA, UCG, CCU, CCC,...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1983-02, Vol.80 (3), p.687-691 |
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| creator | Konigsberg, William Godson, G. Nigel |
| description | Amino acid sequence and composition data of Escherichia coli dnaG primase protein and its tryptic peptides have confirmed that the dnaG gene contains an unusually high number of codons that are not frequently used in most E. coli genes. In 25 E. coli proteins analyzed the codons AUA, UCG, CCU, CCC, ACG, CAA, AAT, and AGG are infrequently used, occurring as 4% of the total codons in the reading frame and 11% and 10% in the nonreading frames. In dnaG they occur as 11% in the reading frame and 12% in the nonreading frames. The rpsU and rpoD genes, which flank the dnaG gene [Smiley, B. L., Lupski, J. R., Svec, P. S., McMacken, R. & Godson, G. N. (1982) Proc. Natl. Acad. Sci. USA 79, 4550-4554], however, have normal codon usage. Translational modulation using isoaccepting tRNA availability may therefore be part of the mechanism of keeping the dnaG gene expression low, while expression of the adjacent rpsU and rpoD genes on the same mRNA transcript is high. |
| doi_str_mv | 10.1073/pnas.80.3.687 |
| format | Article |
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Translational modulation using isoaccepting tRNA availability may therefore be part of the mechanism of keeping the dnaG gene expression low, while expression of the adjacent rpsU and rpoD genes on the same mRNA transcript is high.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.80.3.687</identifier><identifier>PMID: 6338495</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Amino Acid Sequence ; Amino acids ; Bacterial Proteins - genetics ; Base Sequence ; Biochemistry ; Codon ; Codons ; DNA Primase ; Escherichia coli - genetics ; Genes ; Genes, Bacterial ; Genes, Regulator ; Genetic Code ; Gin ; Messenger RNA ; Nucleotide sequences ; Reading frames ; Reading tables ; Repressor Proteins - genetics ; RNA Nucleotidyltransferases - genetics ; Synonyms ; Transcription Factors - genetics</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1983-02, Vol.80 (3), p.687-691</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c451t-5724a696c4b1d5fdf7c32e7ceeb8d11b8f13aed8346561077a5ad8cd2358d5843</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/80/3.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/13735$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/13735$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6338495$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Konigsberg, William</creatorcontrib><creatorcontrib>Godson, G. Nigel</creatorcontrib><title>Evidence for Use of Rare Codons in the dnaG Gene and Other Regulatory Genes of Escherichia coli</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Amino acid sequence and composition data of Escherichia coli dnaG primase protein and its tryptic peptides have confirmed that the dnaG gene contains an unusually high number of codons that are not frequently used in most E. coli genes. In 25 E. coli proteins analyzed the codons AUA, UCG, CCU, CCC, ACG, CAA, AAT, and AGG are infrequently used, occurring as 4% of the total codons in the reading frame and 11% and 10% in the nonreading frames. In dnaG they occur as 11% in the reading frame and 12% in the nonreading frames. The rpsU and rpoD genes, which flank the dnaG gene [Smiley, B. L., Lupski, J. R., Svec, P. S., McMacken, R. & Godson, G. N. (1982) Proc. Natl. Acad. Sci. USA 79, 4550-4554], however, have normal codon usage. Translational modulation using isoaccepting tRNA availability may therefore be part of the mechanism of keeping the dnaG gene expression low, while expression of the adjacent rpsU and rpoD genes on the same mRNA transcript is high.</description><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Bacterial Proteins - genetics</subject><subject>Base Sequence</subject><subject>Biochemistry</subject><subject>Codon</subject><subject>Codons</subject><subject>DNA Primase</subject><subject>Escherichia coli - genetics</subject><subject>Genes</subject><subject>Genes, Bacterial</subject><subject>Genes, Regulator</subject><subject>Genetic Code</subject><subject>Gin</subject><subject>Messenger RNA</subject><subject>Nucleotide sequences</subject><subject>Reading frames</subject><subject>Reading tables</subject><subject>Repressor Proteins - genetics</subject><subject>RNA Nucleotidyltransferases - genetics</subject><subject>Synonyms</subject><subject>Transcription Factors - genetics</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcFv0zAUhy0EGmVw5IJA8gVuKXaenTgHDqgqBWnSpImdLdd-WT2ldrGTif33OLQbcOFkyd_3s9_Tj5DXnC05a-HjIZi8VGwJy0a1T8iCs45XjejYU7JgrG4rJWrxnLzI-ZYx1knFzshZA6BEJxdEr--8w2CR9jHR64w09vTKJKSr6GLI1Ac67pC6YDZ0gwGpCY5elqtEr_BmGswY0_1vkufoOtuCvN15Q20c_EvyrDdDxlen85xcf1l_X32tLi4331afLyorJB8r2dbCNF1jxZY72bu-tVBjaxG3ynG-VT0Hg06BaGRT1m6NNE5ZV4NUTioB5-TT8d3DtN2jsxjGZAZ9SH5v0r2Oxut_SfA7fRPvNHQgxJz_cMqn-GPCPOq9zxaHwQSMU9aKgVIdNEWsjqJNMeeE_eMfnOm5ED0XUnwNuhRS_Hd_D_Zonxoo_P2Jz7EH-hDX_TQMI_4ci_f2P17Bb474NpdC_swELUj4Bacpp8k</recordid><startdate>19830201</startdate><enddate>19830201</enddate><creator>Konigsberg, William</creator><creator>Godson, G. Nigel</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19830201</creationdate><title>Evidence for Use of Rare Codons in the dnaG Gene and Other Regulatory Genes of Escherichia coli</title><author>Konigsberg, William ; Godson, G. Nigel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c451t-5724a696c4b1d5fdf7c32e7ceeb8d11b8f13aed8346561077a5ad8cd2358d5843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>Bacterial Proteins - genetics</topic><topic>Base Sequence</topic><topic>Biochemistry</topic><topic>Codon</topic><topic>Codons</topic><topic>DNA Primase</topic><topic>Escherichia coli - genetics</topic><topic>Genes</topic><topic>Genes, Bacterial</topic><topic>Genes, Regulator</topic><topic>Genetic Code</topic><topic>Gin</topic><topic>Messenger RNA</topic><topic>Nucleotide sequences</topic><topic>Reading frames</topic><topic>Reading tables</topic><topic>Repressor Proteins - genetics</topic><topic>RNA Nucleotidyltransferases - genetics</topic><topic>Synonyms</topic><topic>Transcription Factors - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Konigsberg, William</creatorcontrib><creatorcontrib>Godson, G. Nigel</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Konigsberg, William</au><au>Godson, G. Nigel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evidence for Use of Rare Codons in the dnaG Gene and Other Regulatory Genes of Escherichia coli</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1983-02-01</date><risdate>1983</risdate><volume>80</volume><issue>3</issue><spage>687</spage><epage>691</epage><pages>687-691</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Amino acid sequence and composition data of Escherichia coli dnaG primase protein and its tryptic peptides have confirmed that the dnaG gene contains an unusually high number of codons that are not frequently used in most E. coli genes. In 25 E. coli proteins analyzed the codons AUA, UCG, CCU, CCC, ACG, CAA, AAT, and AGG are infrequently used, occurring as 4% of the total codons in the reading frame and 11% and 10% in the nonreading frames. In dnaG they occur as 11% in the reading frame and 12% in the nonreading frames. The rpsU and rpoD genes, which flank the dnaG gene [Smiley, B. L., Lupski, J. R., Svec, P. S., McMacken, R. & Godson, G. N. (1982) Proc. Natl. Acad. Sci. USA 79, 4550-4554], however, have normal codon usage. Translational modulation using isoaccepting tRNA availability may therefore be part of the mechanism of keeping the dnaG gene expression low, while expression of the adjacent rpsU and rpoD genes on the same mRNA transcript is high.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>6338495</pmid><doi>10.1073/pnas.80.3.687</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1983-02, Vol.80 (3), p.687-691 |
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| source | MEDLINE; Jstor Complete Legacy; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Amino Acid Sequence Amino acids Bacterial Proteins - genetics Base Sequence Biochemistry Codon Codons DNA Primase Escherichia coli - genetics Genes Genes, Bacterial Genes, Regulator Genetic Code Gin Messenger RNA Nucleotide sequences Reading frames Reading tables Repressor Proteins - genetics RNA Nucleotidyltransferases - genetics Synonyms Transcription Factors - genetics |
| title | Evidence for Use of Rare Codons in the dnaG Gene and Other Regulatory Genes of Escherichia coli |
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