Photoaffinity-labeling and fluorescence-distribution studies of gonadotropin-releasing hormone receptors in ovarian granulosa cells

Photoaffinity labeling of rat ovarian granulosa cells and membrane preparations with a bioactive photoaffinity derivative of gonadotropin-releasing hormone resulted in identification of two specific components with apparent molecular weights of 60,000 and 54,000. Fluorescent visualization of gonadot...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1982-03, Vol.79 (6), p.1747-1750
Hauptverfasser:	Hazum, E, Nimrod, A
Format:	Artikel
Sprache:	eng
Schlagworte:	affinity labelling Affinity Labels Buserelin Cell membranes Cultured cells Female Fluorescence Gels gonadoliberin Gonadotropin-Releasing Hormone - analogs & derivatives Gonadotropin-Releasing Hormone - metabolism Granulosa cells Granulosa Cells - metabolism Hormones Hormones - metabolism Humans Internalization LHRH receptors Microscopy, Fluorescence Molecular Weight ovary rats Receptors Receptors, Cell Surface - isolation & purification Receptors, Cell Surface - metabolism Receptors, LHRH Sex hormones
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