Properties of a Saccharomyces cerevisiae mtDNA Segment Conferring High-Frequency Yeast Transformation
The bakers' yeast Saccharomyces cerevisiae is a facultative anaerobe, tolerant to mutations in its mitochondrial genome. Individual cytoplasmic petite mutants retain genetic information derived from any portion of the parental mtDNA, prompting questions concerning distribution of the DNA replic...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1982-03, Vol.79 (5), p.1578-1582 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Hyman, Bradley C. Cramer, Jane Harris Rownd, Robert H. |
| description | The bakers' yeast Saccharomyces cerevisiae is a facultative anaerobe, tolerant to mutations in its mitochondrial genome. Individual cytoplasmic petite mutants retain genetic information derived from any portion of the parental mtDNA, prompting questions concerning distribution of the DNA replication origin(s) on the yeast mitochondrial genome. The experiments described in this paper were designed to test the possibility of using high-frequency yeast transformation as a selection for yeast mtDNA sequences conferring autonomously replicating function. A complete petite mitochondrial genome was inserted into the yeast vector YIp5, and the hybrid plasmid (YRMp1) was used to transform yeast. YRMp1 promoted high-frequency transformation of both wild-type yeast cells and petite mutant hosts lacking mtDNA and was maintained in each of these strains as a high-copy-number extrachromosomal element. The stability and copy-number properties of YRMp1 are similar to those of YRp12, a recombinant plasmid containing a yeast chromosomal autonomously replicating sequence. |
| doi_str_mv | 10.1073/pnas.79.5.1578 |
| format | Article |
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Individual cytoplasmic petite mutants retain genetic information derived from any portion of the parental mtDNA, prompting questions concerning distribution of the DNA replication origin(s) on the yeast mitochondrial genome. The experiments described in this paper were designed to test the possibility of using high-frequency yeast transformation as a selection for yeast mtDNA sequences conferring autonomously replicating function. A complete petite mitochondrial genome was inserted into the yeast vector YIp5, and the hybrid plasmid (YRMp1) was used to transform yeast. YRMp1 promoted high-frequency transformation of both wild-type yeast cells and petite mutant hosts lacking mtDNA and was maintained in each of these strains as a high-copy-number extrachromosomal element. The stability and copy-number properties of YRMp1 are similar to those of YRp12, a recombinant plasmid containing a yeast chromosomal autonomously replicating sequence.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.79.5.1578</identifier><identifier>PMID: 7041124</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Cloning, Molecular ; DNA ; DNA Replication ; DNA, Fungal - genetics ; DNA, Mitochondrial - genetics ; Extrachromosomal Inheritance ; Genetic mutation ; Genetic vectors ; Genomes ; Haploidy ; Microbial genetics ; Mitochondrial DNA ; Phenotype ; Plasmids ; Replication origin ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - genetics ; Transformation, Genetic ; Yeasts</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1982-03, Vol.79 (5), p.1578-1582</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4028-5c7652140dec3834efd7db8e4b5c583f28f19baa185a739aac45005cd765a2f73</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/79/5.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/11820$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/11820$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7041124$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hyman, Bradley C.</creatorcontrib><creatorcontrib>Cramer, Jane Harris</creatorcontrib><creatorcontrib>Rownd, Robert H.</creatorcontrib><title>Properties of a Saccharomyces cerevisiae mtDNA Segment Conferring High-Frequency Yeast Transformation</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The bakers' yeast Saccharomyces cerevisiae is a facultative anaerobe, tolerant to mutations in its mitochondrial genome. Individual cytoplasmic petite mutants retain genetic information derived from any portion of the parental mtDNA, prompting questions concerning distribution of the DNA replication origin(s) on the yeast mitochondrial genome. The experiments described in this paper were designed to test the possibility of using high-frequency yeast transformation as a selection for yeast mtDNA sequences conferring autonomously replicating function. A complete petite mitochondrial genome was inserted into the yeast vector YIp5, and the hybrid plasmid (YRMp1) was used to transform yeast. YRMp1 promoted high-frequency transformation of both wild-type yeast cells and petite mutant hosts lacking mtDNA and was maintained in each of these strains as a high-copy-number extrachromosomal element. The stability and copy-number properties of YRMp1 are similar to those of YRp12, a recombinant plasmid containing a yeast chromosomal autonomously replicating sequence.</description><subject>Cloning, Molecular</subject><subject>DNA</subject><subject>DNA Replication</subject><subject>DNA, Fungal - genetics</subject><subject>DNA, Mitochondrial - genetics</subject><subject>Extrachromosomal Inheritance</subject><subject>Genetic mutation</subject><subject>Genetic vectors</subject><subject>Genomes</subject><subject>Haploidy</subject><subject>Microbial genetics</subject><subject>Mitochondrial DNA</subject><subject>Phenotype</subject><subject>Plasmids</subject><subject>Replication origin</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Transformation, Genetic</subject><subject>Yeasts</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFvEzEQhS0EKmnhygEJaU_cdhnv2rH3wKEKlCJVgNRy4GRNnHHiancd7E1F_n0dJYQgIXGyNO994zd6jL3iUHFQzbv1gKlSbSUrLpV-wiYcWl5ORQtP2QSgVqUWtXjOzlO6B4BWajhjZwoE57WYMPoWw5ri6CkVwRVY3KK1K4yh39o8shTpwSePVPTjhy-XxS0texrGYhYGRzH6YVlc--WqvIr0c0OD3RY_CNNY3EUckguxx9GH4QV75rBL9PLwXrDvVx_vZtflzddPn2eXN6UVUOtSWjWVNRewINvoRpBbqMVck5hLK3Xjau14O0fkWqJqWkQrJIC0i4xh7VRzwd7v9643854WNieN2Jl19D3GrQnozd_K4FdmGR5MI6bAdebfHvgY8jlpNL1PlroOBwqbZJSAnKuG_xq5FC1XTZ2N1d5oY0gpkjuG4WB2BZpdgUa1RppdgRl4c3rC0X5o7OTnHXdUf_PGbbpupF_jyaJ_GrP-eq_fpzHEP7G4zvc9AqPkucA</recordid><startdate>19820301</startdate><enddate>19820301</enddate><creator>Hyman, Bradley C.</creator><creator>Cramer, Jane Harris</creator><creator>Rownd, Robert H.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19820301</creationdate><title>Properties of a Saccharomyces cerevisiae mtDNA Segment Conferring High-Frequency Yeast Transformation</title><author>Hyman, Bradley C. ; Cramer, Jane Harris ; Rownd, Robert H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4028-5c7652140dec3834efd7db8e4b5c583f28f19baa185a739aac45005cd765a2f73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>Cloning, Molecular</topic><topic>DNA</topic><topic>DNA Replication</topic><topic>DNA, Fungal - genetics</topic><topic>DNA, Mitochondrial - genetics</topic><topic>Extrachromosomal Inheritance</topic><topic>Genetic mutation</topic><topic>Genetic vectors</topic><topic>Genomes</topic><topic>Haploidy</topic><topic>Microbial genetics</topic><topic>Mitochondrial DNA</topic><topic>Phenotype</topic><topic>Plasmids</topic><topic>Replication origin</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Transformation, Genetic</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hyman, Bradley C.</creatorcontrib><creatorcontrib>Cramer, Jane Harris</creatorcontrib><creatorcontrib>Rownd, Robert H.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hyman, Bradley C.</au><au>Cramer, Jane Harris</au><au>Rownd, Robert H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Properties of a Saccharomyces cerevisiae mtDNA Segment Conferring High-Frequency Yeast Transformation</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1982-03-01</date><risdate>1982</risdate><volume>79</volume><issue>5</issue><spage>1578</spage><epage>1582</epage><pages>1578-1582</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>The bakers' yeast Saccharomyces cerevisiae is a facultative anaerobe, tolerant to mutations in its mitochondrial genome. Individual cytoplasmic petite mutants retain genetic information derived from any portion of the parental mtDNA, prompting questions concerning distribution of the DNA replication origin(s) on the yeast mitochondrial genome. The experiments described in this paper were designed to test the possibility of using high-frequency yeast transformation as a selection for yeast mtDNA sequences conferring autonomously replicating function. A complete petite mitochondrial genome was inserted into the yeast vector YIp5, and the hybrid plasmid (YRMp1) was used to transform yeast. YRMp1 promoted high-frequency transformation of both wild-type yeast cells and petite mutant hosts lacking mtDNA and was maintained in each of these strains as a high-copy-number extrachromosomal element. 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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1982-03, Vol.79 (5), p.1578-1582 |
| issn | 0027-8424 1091-6490 |
| language | eng |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Cloning, Molecular DNA DNA Replication DNA, Fungal - genetics DNA, Mitochondrial - genetics Extrachromosomal Inheritance Genetic mutation Genetic vectors Genomes Haploidy Microbial genetics Mitochondrial DNA Phenotype Plasmids Replication origin Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Transformation, Genetic Yeasts |
| title | Properties of a Saccharomyces cerevisiae mtDNA Segment Conferring High-Frequency Yeast Transformation |
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