Activation of Related Transforming Genes in Mouse and Human Mammary Carcinomas
High molecular weight DNAs of five tumors induced by mouse mammary tumor virus (MMTV), two mouse mammary tumors induced by a chemical carcinogen, and one human mammary tumor cell line (MCF-7) were assayed for the presence of transmissible activated transforming genes by transfection of NIH 3T3 mouse...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1981-08, Vol.78 (8), p.5185-5189 |
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| creator | Lane, Mary-Ann Sainten, Adrienne Cooper, Geoffrey M. |
| description | High molecular weight DNAs of five tumors induced by mouse mammary tumor virus (MMTV), two mouse mammary tumors induced by a chemical carcinogen, and one human mammary tumor cell line (MCF-7) were assayed for the presence of transmissible activated transforming genes by transfection of NIH 3T3 mouse cells. DNAs of all five MMTV-induced tumors, one chemical carcinogen-induced tumor, and the human tumor cell line induced transformation with high efficiencies (≈ 0.2 transformant per μ g of DNA). NIH cells transformed by DNAs of MMTV-induced tumors did not contain exogenous MMTV DNA sequences, indicating that MMTV-induced mammary carcinomas contained activated cellular transforming genes that were not linked to viral DNA. The transforming activities of DNAs of all five MMTV-induced tumors, the chemical carcinogen-induced mouse tumor, and the human tumor cell line were inactivated by digestion with the restriction endonucleases Pvu II and Sac I, but not by BamHI, EcoRI, HindIII, Kpn I, or Xho I. These results indicate that the same or closely related transforming genes were activated in six different mouse mammary carcinomas, induced by either MMTV or a chemical carcinogen, and in a human mammary carcinoma cell line. |
| doi_str_mv | 10.1073/pnas.78.8.5185 |
| format | Article |
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DNAs of all five MMTV-induced tumors, one chemical carcinogen-induced tumor, and the human tumor cell line induced transformation with high efficiencies (≈ 0.2 transformant per μ g of DNA). NIH cells transformed by DNAs of MMTV-induced tumors did not contain exogenous MMTV DNA sequences, indicating that MMTV-induced mammary carcinomas contained activated cellular transforming genes that were not linked to viral DNA. The transforming activities of DNAs of all five MMTV-induced tumors, the chemical carcinogen-induced mouse tumor, and the human tumor cell line were inactivated by digestion with the restriction endonucleases Pvu II and Sac I, but not by BamHI, EcoRI, HindIII, Kpn I, or Xho I. 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DNAs of all five MMTV-induced tumors, one chemical carcinogen-induced tumor, and the human tumor cell line induced transformation with high efficiencies (≈ 0.2 transformant per μ g of DNA). NIH cells transformed by DNAs of MMTV-induced tumors did not contain exogenous MMTV DNA sequences, indicating that MMTV-induced mammary carcinomas contained activated cellular transforming genes that were not linked to viral DNA. The transforming activities of DNAs of all five MMTV-induced tumors, the chemical carcinogen-induced mouse tumor, and the human tumor cell line were inactivated by digestion with the restriction endonucleases Pvu II and Sac I, but not by BamHI, EcoRI, HindIII, Kpn I, or Xho I. These results indicate that the same or closely related transforming genes were activated in six different mouse mammary carcinomas, induced by either MMTV or a chemical carcinogen, and in a human mammary carcinoma cell line.</description><subject>3T3 cells</subject><subject>9,10-Dimethyl-1,2-benzanthracene - pharmacology</subject><subject>Animals</subject><subject>BALB 3T3 cells</subject><subject>Breast Neoplasms - genetics</subject><subject>Carcinoma</subject><subject>Cell Line</subject><subject>Cell Transformation, Neoplastic</subject><subject>Cells, Cultured</subject><subject>DNA Restriction Enzymes - metabolism</subject><subject>DNA, Neoplasm - genetics</subject><subject>DNA, Viral - genetics</subject><subject>Female</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Humans</subject><subject>Mammary Neoplasms, Experimental - genetics</subject><subject>Mammary Tumor Virus, Mouse - genetics</subject><subject>Mice</subject><subject>Neoplastic cell transformation</subject><subject>NIH 3T3 cells</subject><subject>Oncogenes</subject><subject>Transformed cell line</subject><subject>Tumor cell line</subject><subject>Tumors</subject><subject>Viruses</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc9rFDEUx4NY6lq9ehCEnLzN9CWZmWQOHspiW6FVkHoO2fyoKTPJmmSK_vedYddlBQ89hcf3-3n5Pr4IvSNQE-DsfBtUrrmoRd0S0b5AKwI9qbqmh5doBUB5JRravEKvc34AgL4VcIpOO8op7dkKfb3QxT-q4mPA0eHvdlDFGnyXVMguptGHe3xlg83YB3wbp2yxCgZfT6OaZzWOKv3Ba5W0D3FU-Q06cWrI9u3-PUM_Lj_fra-rm29XX9YXN5WeU5ZKm00nuNa0M8IaIIo4Bx2FRoMhVjva6QY4aZzR3FHHO2H0Rmw0by0RuhXsDH3a7d1Om9EabUNJapDb5JdAMiov_1WC_ynv46NkFFjHZ_7jnk_x12RzkaPP2g6DCnY-UpK2IYyJ5hlGRgQIMhvrnVGnmHOy7hCGgFyakktTkgsp5NLUDHw4PuFg31dz9PPC_VUPvHTTMBT7uxwt-q9x1t_v9IdcYjqK1ULPngBQWLGc</recordid><startdate>19810801</startdate><enddate>19810801</enddate><creator>Lane, Mary-Ann</creator><creator>Sainten, Adrienne</creator><creator>Cooper, Geoffrey M.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7TM</scope><scope>7U7</scope><scope>C1K</scope><scope>5PM</scope></search><sort><creationdate>19810801</creationdate><title>Activation of Related Transforming Genes in Mouse and Human Mammary Carcinomas</title><author>Lane, Mary-Ann ; Sainten, Adrienne ; Cooper, Geoffrey M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c518t-cdb687cc26d8ed01a1ff06204c0d1ecf26c40714fdc7f2f768dcb8bc75e18c583</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1981</creationdate><topic>3T3 cells</topic><topic>9,10-Dimethyl-1,2-benzanthracene - pharmacology</topic><topic>Animals</topic><topic>BALB 3T3 cells</topic><topic>Breast Neoplasms - genetics</topic><topic>Carcinoma</topic><topic>Cell Line</topic><topic>Cell Transformation, Neoplastic</topic><topic>Cells, Cultured</topic><topic>DNA Restriction Enzymes - metabolism</topic><topic>DNA, Neoplasm - genetics</topic><topic>DNA, Viral - genetics</topic><topic>Female</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Humans</topic><topic>Mammary Neoplasms, Experimental - genetics</topic><topic>Mammary Tumor Virus, Mouse - genetics</topic><topic>Mice</topic><topic>Neoplastic cell transformation</topic><topic>NIH 3T3 cells</topic><topic>Oncogenes</topic><topic>Transformed cell line</topic><topic>Tumor cell line</topic><topic>Tumors</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lane, Mary-Ann</creatorcontrib><creatorcontrib>Sainten, Adrienne</creatorcontrib><creatorcontrib>Cooper, Geoffrey M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lane, Mary-Ann</au><au>Sainten, Adrienne</au><au>Cooper, Geoffrey M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Activation of Related Transforming Genes in Mouse and Human Mammary Carcinomas</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1981-08-01</date><risdate>1981</risdate><volume>78</volume><issue>8</issue><spage>5185</spage><epage>5189</epage><pages>5185-5189</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>High molecular weight DNAs of five tumors induced by mouse mammary tumor virus (MMTV), two mouse mammary tumors induced by a chemical carcinogen, and one human mammary tumor cell line (MCF-7) were assayed for the presence of transmissible activated transforming genes by transfection of NIH 3T3 mouse cells. DNAs of all five MMTV-induced tumors, one chemical carcinogen-induced tumor, and the human tumor cell line induced transformation with high efficiencies (≈ 0.2 transformant per μ g of DNA). NIH cells transformed by DNAs of MMTV-induced tumors did not contain exogenous MMTV DNA sequences, indicating that MMTV-induced mammary carcinomas contained activated cellular transforming genes that were not linked to viral DNA. The transforming activities of DNAs of all five MMTV-induced tumors, the chemical carcinogen-induced mouse tumor, and the human tumor cell line were inactivated by digestion with the restriction endonucleases Pvu II and Sac I, but not by BamHI, EcoRI, HindIII, Kpn I, or Xho I. 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| subjects | 3T3 cells 9,10-Dimethyl-1,2-benzanthracene - pharmacology Animals BALB 3T3 cells Breast Neoplasms - genetics Carcinoma Cell Line Cell Transformation, Neoplastic Cells, Cultured DNA Restriction Enzymes - metabolism DNA, Neoplasm - genetics DNA, Viral - genetics Female Gene Expression Regulation - drug effects Humans Mammary Neoplasms, Experimental - genetics Mammary Tumor Virus, Mouse - genetics Mice Neoplastic cell transformation NIH 3T3 cells Oncogenes Transformed cell line Tumor cell line Tumors Viruses |
| title | Activation of Related Transforming Genes in Mouse and Human Mammary Carcinomas |
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