Increase in Proliferative Markers after Inhibition of Transglutaminase
Cystamine inhibited transglutaminase activity (R-glutaminyl-peptide:amine γ -glutamyltransferase, EC 2.3.2.13) of proliferating WI-38 cells in a dose-dependent manner over the concentration range 0.005-0.25 mM when added to the culture medium. The ε -(γ -glutamyl)lysine content in the cells was decr...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1981-08, Vol.78 (8), p.5005-5008 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 78 |
| creator | Birckbichler, P. J. Orr, G. R. Patterson, M. K. Conway, E. Carter, H. A. |
| description | Cystamine inhibited transglutaminase activity (R-glutaminyl-peptide:amine γ -glutamyltransferase, EC 2.3.2.13) of proliferating WI-38 cells in a dose-dependent manner over the concentration range 0.005-0.25 mM when added to the culture medium. The ε -(γ -glutamyl)lysine content in the cells was decreased and several proliferation markers were enhanced. ``Nonmitotic'' cells were stimulated by cystamine (about 25% of that observed with 10% fetal bovine serum) to undergo DNA synthesis with subsequent increases in nuclei number. Numerous other disulfides, thiols, and amines were ineffective when added to culture medium. The findings are supportive of the concept that growth control involves a relationship between isopeptide crosslinks and proliferation. |
| doi_str_mv | 10.1073/pnas.78.8.5005 |
| format | Article |
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The findings are supportive of the concept that growth control involves a relationship between isopeptide crosslinks and proliferation.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.78.8.5005</identifier><identifier>PMID: 6117852</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Amines ; Cell cycle ; Cell Division - drug effects ; Cell growth ; Cell nucleus ; Cells ; Cells, Cultured ; Cultured cells ; Cystamine - pharmacology ; Disulfides ; Disulfides - pharmacology ; DNA Replication - drug effects ; Enzymes ; gamma-Glutamyltransferase - antagonists & inhibitors ; Humans ; L cells ; Sulfhydryl Compounds - pharmacology ; Ungulates</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1981-08, Vol.78 (8), p.5005-5008</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c522t-23baceeb0700a8789708ca653cb674d066b58c090a9bcd03dbcbc31d208403e13</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/78/8.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/10471$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/10471$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,724,777,781,800,882,27905,27906,53772,53774,57998,58231</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6117852$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Birckbichler, P. J.</creatorcontrib><creatorcontrib>Orr, G. R.</creatorcontrib><creatorcontrib>Patterson, M. K.</creatorcontrib><creatorcontrib>Conway, E.</creatorcontrib><creatorcontrib>Carter, H. A.</creatorcontrib><title>Increase in Proliferative Markers after Inhibition of Transglutaminase</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Cystamine inhibited transglutaminase activity (R-glutaminyl-peptide:amine γ -glutamyltransferase, EC 2.3.2.13) of proliferating WI-38 cells in a dose-dependent manner over the concentration range 0.005-0.25 mM when added to the culture medium. The ε -(γ -glutamyl)lysine content in the cells was decreased and several proliferation markers were enhanced. ``Nonmitotic'' cells were stimulated by cystamine (about 25% of that observed with 10% fetal bovine serum) to undergo DNA synthesis with subsequent increases in nuclei number. Numerous other disulfides, thiols, and amines were ineffective when added to culture medium. The findings are supportive of the concept that growth control involves a relationship between isopeptide crosslinks and proliferation.</description><subject>Amines</subject><subject>Cell cycle</subject><subject>Cell Division - drug effects</subject><subject>Cell growth</subject><subject>Cell nucleus</subject><subject>Cells</subject><subject>Cells, Cultured</subject><subject>Cultured cells</subject><subject>Cystamine - pharmacology</subject><subject>Disulfides</subject><subject>Disulfides - pharmacology</subject><subject>DNA Replication - drug effects</subject><subject>Enzymes</subject><subject>gamma-Glutamyltransferase - antagonists & inhibitors</subject><subject>Humans</subject><subject>L cells</subject><subject>Sulfhydryl Compounds - pharmacology</subject><subject>Ungulates</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptUM9L5DAUDouLzo5e9yAIPXlr96Vpm_TgQUTXAZfdg3sOSfqq0U4yJqms__1OmXEYQXjwDt9PPkK-UygocPZj5VQsuChEUQPUX8iMQkvzpmrhgMwASp6LqqyOyLcYnwCgrQUcksOGUi7qckZuFs4EVBEz67I_wQ-2x6CSfcXslwrPGGKm-oQhW7hHq22y3mW-z-6DcvFhGJNa2nUBPCZfezVEPNn-Ofl7c31_dZvf_f65uLq8y01dlikvmVYGUQMHUIKLloMwqqmZ0Q2vOmgaXQsDLahWmw5Yp402jHYliAoYUjYnFxvf1aiX2Bl0KahBroJdqvAmvbLyI-Lso3zwr5KVMN2cnG_1wb-MGJNc2mhwGJRDP0bJWdPymrE1sdgQTfAxBux3GRTkNLychpdcSCGn4deCs_1mO_p26b3kSfeO7vSyH4ch4b-0Z_QpcY2fbvCnmHzYq1Vxyv4D4ZOhMw</recordid><startdate>19810801</startdate><enddate>19810801</enddate><creator>Birckbichler, P. J.</creator><creator>Orr, G. R.</creator><creator>Patterson, M. K.</creator><creator>Conway, E.</creator><creator>Carter, H. A.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19810801</creationdate><title>Increase in Proliferative Markers after Inhibition of Transglutaminase</title><author>Birckbichler, P. J. ; Orr, G. R. ; Patterson, M. K. ; Conway, E. ; Carter, H. 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J.</creatorcontrib><creatorcontrib>Orr, G. R.</creatorcontrib><creatorcontrib>Patterson, M. K.</creatorcontrib><creatorcontrib>Conway, E.</creatorcontrib><creatorcontrib>Carter, H. A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Birckbichler, P. J.</au><au>Orr, G. R.</au><au>Patterson, M. K.</au><au>Conway, E.</au><au>Carter, H. A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Increase in Proliferative Markers after Inhibition of Transglutaminase</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1981-08-01</date><risdate>1981</risdate><volume>78</volume><issue>8</issue><spage>5005</spage><epage>5008</epage><pages>5005-5008</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Cystamine inhibited transglutaminase activity (R-glutaminyl-peptide:amine γ -glutamyltransferase, EC 2.3.2.13) of proliferating WI-38 cells in a dose-dependent manner over the concentration range 0.005-0.25 mM when added to the culture medium. The ε -(γ -glutamyl)lysine content in the cells was decreased and several proliferation markers were enhanced. ``Nonmitotic'' cells were stimulated by cystamine (about 25% of that observed with 10% fetal bovine serum) to undergo DNA synthesis with subsequent increases in nuclei number. Numerous other disulfides, thiols, and amines were ineffective when added to culture medium. The findings are supportive of the concept that growth control involves a relationship between isopeptide crosslinks and proliferation.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>6117852</pmid><doi>10.1073/pnas.78.8.5005</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1981-08, Vol.78 (8), p.5005-5008 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_pnas_primary_78_8_5005_fulltext |
| source | MEDLINE; Jstor Complete Legacy; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Amines Cell cycle Cell Division - drug effects Cell growth Cell nucleus Cells Cells, Cultured Cultured cells Cystamine - pharmacology Disulfides Disulfides - pharmacology DNA Replication - drug effects Enzymes gamma-Glutamyltransferase - antagonists & inhibitors Humans L cells Sulfhydryl Compounds - pharmacology Ungulates |
| title | Increase in Proliferative Markers after Inhibition of Transglutaminase |
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