Partial Characterization of γ (Immune) Interferon mRNA Extracted from Human Lymphocytes
γ (immune) interferon (IFN-γ ) was induced in cultures of fresh human lymphocytes by combined treatment with a phorbol ester (12-O-tetradecanoylphorbol 13-acetate, TPA) and the T cell mitogen phytohemagglutinin (PHA). Compared to the IFN-γ yields obtained with PHA induction alone, the inclusion of T...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1981-06, Vol.78 (6), p.3469-3472 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Taniguchi, Tadatsugu Roy H. L. Pang Yip, Y. K. Henriksen, Dorothy Vilček, Jan |
| description | γ (immune) interferon (IFN-γ ) was induced in cultures of fresh human lymphocytes by combined treatment with a phorbol ester (12-O-tetradecanoylphorbol 13-acetate, TPA) and the T cell mitogen phytohemagglutinin (PHA). Compared to the IFN-γ yields obtained with PHA induction alone, the inclusion of TPA caused a significant enhancement of IFN-γ production. Poly(A)-containing mRNA was isolated from mononuclear cells induced with TPA and PHA. Injected into Xenopus laevis oocytes, this mRNA preparation gave rise to IFN activity with characteristic properties of human IFN-γ . Sucrose density gradient centrifugation analysis showed that IFN-γ mRNA sedimented at 15 S, suggesting that it contains a total of about 1400 nucleotides. |
| doi_str_mv | 10.1073/pnas.78.6.3469 |
| format | Article |
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Sucrose density gradient centrifugation analysis showed that IFN-γ mRNA sedimented at 15 S, suggesting that it contains a total of about 1400 nucleotides.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.78.6.3469</identifier><identifier>PMID: 6167986</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Animals ; Biochemistry ; Centrifugation ; Complementary DNA ; Cultured cells ; Epitopes ; Female ; Humans ; Interferons ; Interferons - biosynthesis ; Interferons - genetics ; Interferons - immunology ; Lymphocytes ; Lymphocytes - physiology ; Messenger RNA ; Oocytes ; Oocytes - physiology ; Phytohemagglutinins - pharmacology ; RNA ; RNA, Messenger - genetics ; Tetradecanoylphorbol Acetate - pharmacology ; Xenopus laevis</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1981-06, Vol.78 (6), p.3469-3472</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3719-f857705a0b8cd4ea92f4c106b8e2a7a10a69df8128e0dfc7a3fe1ddad9cb74b83</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/78/6.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/10855$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/10855$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6167986$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Taniguchi, Tadatsugu</creatorcontrib><creatorcontrib>Roy H. L. Pang</creatorcontrib><creatorcontrib>Yip, Y. K.</creatorcontrib><creatorcontrib>Henriksen, Dorothy</creatorcontrib><creatorcontrib>Vilček, Jan</creatorcontrib><title>Partial Characterization of γ (Immune) Interferon mRNA Extracted from Human Lymphocytes</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>γ (immune) interferon (IFN-γ ) was induced in cultures of fresh human lymphocytes by combined treatment with a phorbol ester (12-O-tetradecanoylphorbol 13-acetate, TPA) and the T cell mitogen phytohemagglutinin (PHA). Compared to the IFN-γ yields obtained with PHA induction alone, the inclusion of TPA caused a significant enhancement of IFN-γ production. Poly(A)-containing mRNA was isolated from mononuclear cells induced with TPA and PHA. Injected into Xenopus laevis oocytes, this mRNA preparation gave rise to IFN activity with characteristic properties of human IFN-γ . Sucrose density gradient centrifugation analysis showed that IFN-γ mRNA sedimented at 15 S, suggesting that it contains a total of about 1400 nucleotides.</description><subject>Animals</subject><subject>Biochemistry</subject><subject>Centrifugation</subject><subject>Complementary DNA</subject><subject>Cultured cells</subject><subject>Epitopes</subject><subject>Female</subject><subject>Humans</subject><subject>Interferons</subject><subject>Interferons - biosynthesis</subject><subject>Interferons - genetics</subject><subject>Interferons - immunology</subject><subject>Lymphocytes</subject><subject>Lymphocytes - physiology</subject><subject>Messenger RNA</subject><subject>Oocytes</subject><subject>Oocytes - physiology</subject><subject>Phytohemagglutinins - pharmacology</subject><subject>RNA</subject><subject>RNA, Messenger - genetics</subject><subject>Tetradecanoylphorbol Acetate - pharmacology</subject><subject>Xenopus laevis</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkMtO20AUhkcVCALttgskpFlVsLB7xpe5LLpAUYBIEVRVK3U3Oh7PECNforFdJX0t3oNnwiEhChKrs_j-75yjn5CvDEIGIv6-qLENhQx5GCdcfSIjBooFPFFwQEYAkQhkEiXH5KRtHwFApRKOyBFnXCjJR-TvT_RdgSUdz9Gj6awv_mNXNDVtHH1-ohfTqupre0mn9cCc9QOpft1d0cmye83n1Pmmord9hTWdrarFvDGrzrafyaHDsrVftvOU_Lme_B7fBrP7m-n4ahaYWDAVOJkKASlCJk2eWFSRSwwDnkkboUAGyFXuJIukhdwZgbGzLM8xVyYTSSbjU_Jjs3fRZ5XNja2Hv0q98EWFfqUbLPR7Uhdz_dD80zFTqYLBDze-8U3beut2KgO9blivG9ZCaq7XDQ_C-f7BXXxb6cC_bfnae6M7X7u-LDu77PYWfRgc-NmGP7Zd4_fekmkavwCj5Zt2</recordid><startdate>19810601</startdate><enddate>19810601</enddate><creator>Taniguchi, Tadatsugu</creator><creator>Roy H. L. Pang</creator><creator>Yip, Y. K.</creator><creator>Henriksen, Dorothy</creator><creator>Vilček, Jan</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>19810601</creationdate><title>Partial Characterization of γ (Immune) Interferon mRNA Extracted from Human Lymphocytes</title><author>Taniguchi, Tadatsugu ; Roy H. L. Pang ; Yip, Y. K. ; Henriksen, Dorothy ; Vilček, Jan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3719-f857705a0b8cd4ea92f4c106b8e2a7a10a69df8128e0dfc7a3fe1ddad9cb74b83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1981</creationdate><topic>Animals</topic><topic>Biochemistry</topic><topic>Centrifugation</topic><topic>Complementary DNA</topic><topic>Cultured cells</topic><topic>Epitopes</topic><topic>Female</topic><topic>Humans</topic><topic>Interferons</topic><topic>Interferons - biosynthesis</topic><topic>Interferons - genetics</topic><topic>Interferons - immunology</topic><topic>Lymphocytes</topic><topic>Lymphocytes - physiology</topic><topic>Messenger RNA</topic><topic>Oocytes</topic><topic>Oocytes - physiology</topic><topic>Phytohemagglutinins - pharmacology</topic><topic>RNA</topic><topic>RNA, Messenger - genetics</topic><topic>Tetradecanoylphorbol Acetate - pharmacology</topic><topic>Xenopus laevis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Taniguchi, Tadatsugu</creatorcontrib><creatorcontrib>Roy H. L. Pang</creatorcontrib><creatorcontrib>Yip, Y. K.</creatorcontrib><creatorcontrib>Henriksen, Dorothy</creatorcontrib><creatorcontrib>Vilček, Jan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Taniguchi, Tadatsugu</au><au>Roy H. L. Pang</au><au>Yip, Y. K.</au><au>Henriksen, Dorothy</au><au>Vilček, Jan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Partial Characterization of γ (Immune) Interferon mRNA Extracted from Human Lymphocytes</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1981-06-01</date><risdate>1981</risdate><volume>78</volume><issue>6</issue><spage>3469</spage><epage>3472</epage><pages>3469-3472</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>γ (immune) interferon (IFN-γ ) was induced in cultures of fresh human lymphocytes by combined treatment with a phorbol ester (12-O-tetradecanoylphorbol 13-acetate, TPA) and the T cell mitogen phytohemagglutinin (PHA). Compared to the IFN-γ yields obtained with PHA induction alone, the inclusion of TPA caused a significant enhancement of IFN-γ production. Poly(A)-containing mRNA was isolated from mononuclear cells induced with TPA and PHA. Injected into Xenopus laevis oocytes, this mRNA preparation gave rise to IFN activity with characteristic properties of human IFN-γ . Sucrose density gradient centrifugation analysis showed that IFN-γ mRNA sedimented at 15 S, suggesting that it contains a total of about 1400 nucleotides.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>6167986</pmid><doi>10.1073/pnas.78.6.3469</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1981-06, Vol.78 (6), p.3469-3472 |
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| language | eng |
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| source | Jstor Complete Legacy; MEDLINE; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Animals Biochemistry Centrifugation Complementary DNA Cultured cells Epitopes Female Humans Interferons Interferons - biosynthesis Interferons - genetics Interferons - immunology Lymphocytes Lymphocytes - physiology Messenger RNA Oocytes Oocytes - physiology Phytohemagglutinins - pharmacology RNA RNA, Messenger - genetics Tetradecanoylphorbol Acetate - pharmacology Xenopus laevis |
| title | Partial Characterization of γ (Immune) Interferon mRNA Extracted from Human Lymphocytes |
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