Partial Characterization of γ (Immune) Interferon mRNA Extracted from Human Lymphocytes

γ (immune) interferon (IFN-γ ) was induced in cultures of fresh human lymphocytes by combined treatment with a phorbol ester (12-O-tetradecanoylphorbol 13-acetate, TPA) and the T cell mitogen phytohemagglutinin (PHA). Compared to the IFN-γ yields obtained with PHA induction alone, the inclusion of T...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1981-06, Vol.78 (6), p.3469-3472
Hauptverfasser:	Taniguchi, Tadatsugu, Roy H. L. Pang, Yip, Y. K., Henriksen, Dorothy, Vilček, Jan
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biochemistry Centrifugation Complementary DNA Cultured cells Epitopes Female Humans Interferons Interferons - biosynthesis Interferons - genetics Interferons - immunology Lymphocytes Lymphocytes - physiology Messenger RNA Oocytes Oocytes - physiology Phytohemagglutinins - pharmacology RNA RNA, Messenger - genetics Tetradecanoylphorbol Acetate - pharmacology Xenopus laevis
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Beschreibung
Zusammenfassung:	γ (immune) interferon (IFN-γ ) was induced in cultures of fresh human lymphocytes by combined treatment with a phorbol ester (12-O-tetradecanoylphorbol 13-acetate, TPA) and the T cell mitogen phytohemagglutinin (PHA). Compared to the IFN-γ yields obtained with PHA induction alone, the inclusion of TPA caused a significant enhancement of IFN-γ production. Poly(A)-containing mRNA was isolated from mononuclear cells induced with TPA and PHA. Injected into Xenopus laevis oocytes, this mRNA preparation gave rise to IFN activity with characteristic properties of human IFN-γ . Sucrose density gradient centrifugation analysis showed that IFN-γ mRNA sedimented at 15 S, suggesting that it contains a total of about 1400 nucleotides.
ISSN:	0027-8424 1091-6490
DOI:	10.1073/pnas.78.6.3469