Cloning and Expression of the Pst I Restriction-Modification System in Escherichia coli

Here we report the cloning and preliminary characterization of the Pst I restriction-modification system of Providencia stuartii 164. Transformants of Escherichia coli carrying the Pst I gene system inserted into the cloning vector pBR322 were selected on the basis of acquired resistance to bacterio...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1981-03, Vol.78 (3), p.1503-1507
Hauptverfasser:	Walder, Roxanne Y., Hartley, James L., Donelson, John E., Walder, Joseph A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteriophages Base Sequence Biochemistry Cloning, Molecular Deoxyribonuclease HindIII Deoxyribonucleases, Type II Site-Specific DNA DNA Restriction Enzymes - genetics DNA, Recombinant - metabolism Enzymes Escherichia coli - metabolism Gels Genes Infections Insect colonies Molecular Weight Plasmids Proteus - enzymology Providencia - enzymology Providencia - genetics RNA Transformation, Bacterial
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