Regulation of Intracellular Levels of Calmodulin and Tubulin in Normal and Transformed Cells
Transformation of mammalian tissue culture cells by oncogenic viruses results in a 2-fold increase in the intracellular concentration of calmodulin quantitated by radioimmunoassay. The two pairs of companion cell lines used in this study were the Swiss mouse 3T3/simian virus 40-transformed 3T3 cells...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1981-02, Vol.78 (2), p.996-1000 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Chafouleas, James G. Pardue, Robert L. Brinkley, B. R. Dedman, John R. Means, Anthony R. |
| description | Transformation of mammalian tissue culture cells by oncogenic viruses results in a 2-fold increase in the intracellular concentration of calmodulin quantitated by radioimmunoassay. The two pairs of companion cell lines used in this study were the Swiss mouse 3T3/simian virus 40-transformed 3T3 cells and the normal rat kidney (NRK)/Rous sarcoma virus-transformed NRK cells. The increased intracellular levels of calmodulin in the transformed cells are due to a greater increase in the rate of synthesis (3-fold) relative to the change in the rate of degradation (1.4-fold). On the other hand, no increases were observed in tubulin levels as quantitated by a colchicine-binding assay. The lack of change in tubulin concentration was accounted for by a 2-fold increase in the rate of degradation that is compensated by a similar increase in the rate of synthesis. The consequence of such changes in both transformed cell types is a 2-fold increase in the calmodulin-to-tubulin protein ratio relative to that in their nontransformed counterparts. |
| doi_str_mv | 10.1073/pnas.78.2.996 |
| format | Article |
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R. ; Dedman, John R. ; Means, Anthony R.</creator><creatorcontrib>Chafouleas, James G. ; Pardue, Robert L. ; Brinkley, B. R. ; Dedman, John R. ; Means, Anthony R.</creatorcontrib><description>Transformation of mammalian tissue culture cells by oncogenic viruses results in a 2-fold increase in the intracellular concentration of calmodulin quantitated by radioimmunoassay. The two pairs of companion cell lines used in this study were the Swiss mouse 3T3/simian virus 40-transformed 3T3 cells and the normal rat kidney (NRK)/Rous sarcoma virus-transformed NRK cells. The increased intracellular levels of calmodulin in the transformed cells are due to a greater increase in the rate of synthesis (3-fold) relative to the change in the rate of degradation (1.4-fold). On the other hand, no increases were observed in tubulin levels as quantitated by a colchicine-binding assay. The lack of change in tubulin concentration was accounted for by a 2-fold increase in the rate of degradation that is compensated by a similar increase in the rate of synthesis. The consequence of such changes in both transformed cell types is a 2-fold increase in the calmodulin-to-tubulin protein ratio relative to that in their nontransformed counterparts.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.78.2.996</identifier><identifier>PMID: 6262788</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>3T3 cells ; Animals ; Avian Sarcoma Viruses - metabolism ; Calcium-Binding Proteins - metabolism ; Calmodulin - metabolism ; Cell Line ; Cell lines ; Cell Transformation, Viral ; Cells ; Cells, Cultured ; Cultured cells ; Gene expression regulation ; Kidney ; Kinetics ; Methionine - metabolism ; Mice ; Microtubules ; Physiological regulation ; Rats ; Simian virus 40 - metabolism ; Sulfur Radioisotopes ; Swiss 3T3 cells ; Tissue culture techniques ; Transformed cell line ; Tubulin - metabolism</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1981-02, Vol.78 (2), p.996-1000</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c449t-40758cdd057a8bee724619b9ed2810e28830b52ac9a1f02c29d62791348170343</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/78/2.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/10333$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/10333$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6262788$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chafouleas, James G.</creatorcontrib><creatorcontrib>Pardue, Robert L.</creatorcontrib><creatorcontrib>Brinkley, B. R.</creatorcontrib><creatorcontrib>Dedman, John R.</creatorcontrib><creatorcontrib>Means, Anthony R.</creatorcontrib><title>Regulation of Intracellular Levels of Calmodulin and Tubulin in Normal and Transformed Cells</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Transformation of mammalian tissue culture cells by oncogenic viruses results in a 2-fold increase in the intracellular concentration of calmodulin quantitated by radioimmunoassay. The two pairs of companion cell lines used in this study were the Swiss mouse 3T3/simian virus 40-transformed 3T3 cells and the normal rat kidney (NRK)/Rous sarcoma virus-transformed NRK cells. The increased intracellular levels of calmodulin in the transformed cells are due to a greater increase in the rate of synthesis (3-fold) relative to the change in the rate of degradation (1.4-fold). On the other hand, no increases were observed in tubulin levels as quantitated by a colchicine-binding assay. The lack of change in tubulin concentration was accounted for by a 2-fold increase in the rate of degradation that is compensated by a similar increase in the rate of synthesis. The consequence of such changes in both transformed cell types is a 2-fold increase in the calmodulin-to-tubulin protein ratio relative to that in their nontransformed counterparts.</description><subject>3T3 cells</subject><subject>Animals</subject><subject>Avian Sarcoma Viruses - metabolism</subject><subject>Calcium-Binding Proteins - metabolism</subject><subject>Calmodulin - metabolism</subject><subject>Cell Line</subject><subject>Cell lines</subject><subject>Cell Transformation, Viral</subject><subject>Cells</subject><subject>Cells, Cultured</subject><subject>Cultured cells</subject><subject>Gene expression regulation</subject><subject>Kidney</subject><subject>Kinetics</subject><subject>Methionine - metabolism</subject><subject>Mice</subject><subject>Microtubules</subject><subject>Physiological regulation</subject><subject>Rats</subject><subject>Simian virus 40 - metabolism</subject><subject>Sulfur Radioisotopes</subject><subject>Swiss 3T3 cells</subject><subject>Tissue culture techniques</subject><subject>Transformed cell line</subject><subject>Tubulin - metabolism</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UU1LxDAQDaLo-nH0Igq96K3r5KNNcvAgi1-wKIjehJC2qVayzZq0ov_e1F1lvRgCSea9N_Myg9A-hjEGTk_nrQ5jLsZkLGW-hkYYJE5zJmEdjQAITwUjbAtth_AKADITsIk2c5ITLsQIPd2b597qrnFt4urkpu28Lo21MeaTqXk3NgzxibYzV_W2aRPdVslDX3zf4751fqbtIup1G-r4NlUyiTnCLtqotQ1mb3nuoMfLi4fJdTq9u7qZnE_TkjHZpQx4JsqqgoxrURjDCcuxLKSpiMBgiBAUiozoUmpcAymJrKJ7iSkTmANldAedLfLO-yIWL83wC6vmvplp_6mcbtRfpG1e1LN7VxRLSUnUnyz13r31JnRq1oShC7o1rg-KZzllWZ5FYroglt6F4E39WwODGqahhmkoLhRRcRqRf7Rq7Je9bH_ED5f4IPtBV-TH_8Cq7q3tzEcXeQcL3mvonF_xROP6AoP3pvw</recordid><startdate>19810201</startdate><enddate>19810201</enddate><creator>Chafouleas, James G.</creator><creator>Pardue, Robert L.</creator><creator>Brinkley, B. R.</creator><creator>Dedman, John R.</creator><creator>Means, Anthony R.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19810201</creationdate><title>Regulation of Intracellular Levels of Calmodulin and Tubulin in Normal and Transformed Cells</title><author>Chafouleas, James G. ; Pardue, Robert L. ; Brinkley, B. R. ; Dedman, John R. ; Means, Anthony R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c449t-40758cdd057a8bee724619b9ed2810e28830b52ac9a1f02c29d62791348170343</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1981</creationdate><topic>3T3 cells</topic><topic>Animals</topic><topic>Avian Sarcoma Viruses - metabolism</topic><topic>Calcium-Binding Proteins - metabolism</topic><topic>Calmodulin - metabolism</topic><topic>Cell Line</topic><topic>Cell lines</topic><topic>Cell Transformation, Viral</topic><topic>Cells</topic><topic>Cells, Cultured</topic><topic>Cultured cells</topic><topic>Gene expression regulation</topic><topic>Kidney</topic><topic>Kinetics</topic><topic>Methionine - metabolism</topic><topic>Mice</topic><topic>Microtubules</topic><topic>Physiological regulation</topic><topic>Rats</topic><topic>Simian virus 40 - metabolism</topic><topic>Sulfur Radioisotopes</topic><topic>Swiss 3T3 cells</topic><topic>Tissue culture techniques</topic><topic>Transformed cell line</topic><topic>Tubulin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chafouleas, James G.</creatorcontrib><creatorcontrib>Pardue, Robert L.</creatorcontrib><creatorcontrib>Brinkley, B. R.</creatorcontrib><creatorcontrib>Dedman, John R.</creatorcontrib><creatorcontrib>Means, Anthony R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chafouleas, James G.</au><au>Pardue, Robert L.</au><au>Brinkley, B. 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The increased intracellular levels of calmodulin in the transformed cells are due to a greater increase in the rate of synthesis (3-fold) relative to the change in the rate of degradation (1.4-fold). On the other hand, no increases were observed in tubulin levels as quantitated by a colchicine-binding assay. The lack of change in tubulin concentration was accounted for by a 2-fold increase in the rate of degradation that is compensated by a similar increase in the rate of synthesis. The consequence of such changes in both transformed cell types is a 2-fold increase in the calmodulin-to-tubulin protein ratio relative to that in their nontransformed counterparts.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>6262788</pmid><doi>10.1073/pnas.78.2.996</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1981-02, Vol.78 (2), p.996-1000 |
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| source | Jstor Complete Legacy; MEDLINE; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | 3T3 cells Animals Avian Sarcoma Viruses - metabolism Calcium-Binding Proteins - metabolism Calmodulin - metabolism Cell Line Cell lines Cell Transformation, Viral Cells Cells, Cultured Cultured cells Gene expression regulation Kidney Kinetics Methionine - metabolism Mice Microtubules Physiological regulation Rats Simian virus 40 - metabolism Sulfur Radioisotopes Swiss 3T3 cells Tissue culture techniques Transformed cell line Tubulin - metabolism |
| title | Regulation of Intracellular Levels of Calmodulin and Tubulin in Normal and Transformed Cells |
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