Involvement of Trypsin-Like Activity in Binding of Mouse Spermatozoa to Zonae Pellucidae

Work from a number of laboratories has shown that fertilization is blocked in the presence of protease inhibitors, although the specific site of inhibition has not been identified. The present experiments were designed to discriminate between sperm binding to zonae pellucidae as opposed to sperm pen...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 1981-10, Vol.78 (10), p.6231-6235
1. Verfasser: Saling, Patricia M.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 6235
container_issue 10
container_start_page 6231
container_title Proceedings of the National Academy of Sciences - PNAS
container_volume 78
creator Saling, Patricia M.
description Work from a number of laboratories has shown that fertilization is blocked in the presence of protease inhibitors, although the specific site of inhibition has not been identified. The present experiments were designed to discriminate between sperm binding to zonae pellucidae as opposed to sperm penetration through zonae, so as to assess the effect of protease inhibitors on these two distinct events. Exposure of capacitated mouse spermatozoa to a variety of protease inhibitors directed against trypsin blocked sperm binding to zonae in a concentration-dependent manner. A chymotrypsin-directed inhibitor was not capable of blocking sperm binding to zonae. The trypsin inhibitors did not affect sperm penetration through zonae nor gamete membrane fusion if the sperm had established a firm association with the zona surface before addition of the inhibitors. Previous incubation of zona-intact eggs with the inhibitors did not lead to a reduction in sperm binding, indicating that the activity affected by the inhibitors is borne by spermatozoa. Interaction between spermatozoa and the zona surface appeared to be the specific locus of inhibition; sperm binding to zona-free eggs (i.e., binding to the egg plasma membrane) was unaltered by the trypsin inhibitors. These results suggest a reevaluation of the function of proteases in fertilization focusing on their role in initial sperm contact with the zona pellucida.
doi_str_mv 10.1073/pnas.78.10.6231
format Article
fullrecord <record><control><sourceid>jstor_pnas_</sourceid><recordid>TN_cdi_pnas_primary_78_10_6231</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>11041</jstor_id><sourcerecordid>11041</sourcerecordid><originalsourceid>FETCH-LOGICAL-c558t-4d3c16761f20427657f939a15880d23a4b8d6340f8916e9e2629193a3d60b8be3</originalsourceid><addsrcrecordid>eNqFkc1rFDEYxoNY6lo9C4KSkz3NNl-Tj0MPtfhRWFGwgngJ2ZlMTZ1JxiSzuP71zbDbai96Ci_P73l5nzwAPMNoiZGgJ6M3aSlkGZacUPwALDBSuOJMoYdggRARlWSEPQKPU7pGCKlaokNwyBUThPAF-HrhN6Hf2MH6DEMHL-N2TM5XK_fDwrMmu43LW-g8fO186_zVzHwIU7Lw82jjYHL4HQzMAX4L3lj4yfb91LjW2CfgoDN9sk_37xH48vbN5fn7avXx3cX52apq6lrmirW0wVxw3BHEiOC16BRVBtdSopZQw9ay5ZShTirMrbKEE4UVNbTlaC3Xlh6B093ecVoPtm1Kjmh6PUY3mLjVwTh9X_Huu74KG03LF2FS_K_2_hh-TjZlPbjUlBjG25JTCypkjWr8XxDXlGNJWAFPdmATQ0rRdnfHYKTn0vRcmhZynufSiuPF3xnu-H1LRX-512fjrXpvwfE_Ad1NfZ_tr1zI5zvyOuUQ_1yGEcP0Bt6ts_4</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15361824</pqid></control><display><type>article</type><title>Involvement of Trypsin-Like Activity in Binding of Mouse Spermatozoa to Zonae Pellucidae</title><source>MEDLINE</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><source>JSTOR</source><creator>Saling, Patricia M.</creator><creatorcontrib>Saling, Patricia M.</creatorcontrib><description>Work from a number of laboratories has shown that fertilization is blocked in the presence of protease inhibitors, although the specific site of inhibition has not been identified. The present experiments were designed to discriminate between sperm binding to zonae pellucidae as opposed to sperm penetration through zonae, so as to assess the effect of protease inhibitors on these two distinct events. Exposure of capacitated mouse spermatozoa to a variety of protease inhibitors directed against trypsin blocked sperm binding to zonae in a concentration-dependent manner. A chymotrypsin-directed inhibitor was not capable of blocking sperm binding to zonae. The trypsin inhibitors did not affect sperm penetration through zonae nor gamete membrane fusion if the sperm had established a firm association with the zona surface before addition of the inhibitors. Previous incubation of zona-intact eggs with the inhibitors did not lead to a reduction in sperm binding, indicating that the activity affected by the inhibitors is borne by spermatozoa. Interaction between spermatozoa and the zona surface appeared to be the specific locus of inhibition; sperm binding to zona-free eggs (i.e., binding to the egg plasma membrane) was unaltered by the trypsin inhibitors. These results suggest a reevaluation of the function of proteases in fertilization focusing on their role in initial sperm contact with the zona pellucida.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.78.10.6231</identifier><identifier>PMID: 6947226</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Animals ; Cell membranes ; Chemical suspensions ; Female ; Fertilization ; Fertilization in Vitro ; Gametes ; Herpes zoster ; Male ; Mice ; Ova ; Ovum - physiology ; Protease inhibitors ; Protease Inhibitors - pharmacology ; Sperm-Ovum Interactions - drug effects ; Spermatozoa ; Spermatozoa - drug effects ; Spermatozoa - physiology ; Trypsin - metabolism ; Trypsin inhibitors ; trysin ; Zona pellucida ; Zona Pellucida - drug effects ; Zona Pellucida - physiology</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1981-10, Vol.78 (10), p.6231-6235</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c558t-4d3c16761f20427657f939a15880d23a4b8d6340f8916e9e2629193a3d60b8be3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/78/10.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/11041$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/11041$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6947226$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Saling, Patricia M.</creatorcontrib><title>Involvement of Trypsin-Like Activity in Binding of Mouse Spermatozoa to Zonae Pellucidae</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Work from a number of laboratories has shown that fertilization is blocked in the presence of protease inhibitors, although the specific site of inhibition has not been identified. The present experiments were designed to discriminate between sperm binding to zonae pellucidae as opposed to sperm penetration through zonae, so as to assess the effect of protease inhibitors on these two distinct events. Exposure of capacitated mouse spermatozoa to a variety of protease inhibitors directed against trypsin blocked sperm binding to zonae in a concentration-dependent manner. A chymotrypsin-directed inhibitor was not capable of blocking sperm binding to zonae. The trypsin inhibitors did not affect sperm penetration through zonae nor gamete membrane fusion if the sperm had established a firm association with the zona surface before addition of the inhibitors. Previous incubation of zona-intact eggs with the inhibitors did not lead to a reduction in sperm binding, indicating that the activity affected by the inhibitors is borne by spermatozoa. Interaction between spermatozoa and the zona surface appeared to be the specific locus of inhibition; sperm binding to zona-free eggs (i.e., binding to the egg plasma membrane) was unaltered by the trypsin inhibitors. These results suggest a reevaluation of the function of proteases in fertilization focusing on their role in initial sperm contact with the zona pellucida.</description><subject>Animals</subject><subject>Cell membranes</subject><subject>Chemical suspensions</subject><subject>Female</subject><subject>Fertilization</subject><subject>Fertilization in Vitro</subject><subject>Gametes</subject><subject>Herpes zoster</subject><subject>Male</subject><subject>Mice</subject><subject>Ova</subject><subject>Ovum - physiology</subject><subject>Protease inhibitors</subject><subject>Protease Inhibitors - pharmacology</subject><subject>Sperm-Ovum Interactions - drug effects</subject><subject>Spermatozoa</subject><subject>Spermatozoa - drug effects</subject><subject>Spermatozoa - physiology</subject><subject>Trypsin - metabolism</subject><subject>Trypsin inhibitors</subject><subject>trysin</subject><subject>Zona pellucida</subject><subject>Zona Pellucida - drug effects</subject><subject>Zona Pellucida - physiology</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1rFDEYxoNY6lo9C4KSkz3NNl-Tj0MPtfhRWFGwgngJ2ZlMTZ1JxiSzuP71zbDbai96Ci_P73l5nzwAPMNoiZGgJ6M3aSlkGZacUPwALDBSuOJMoYdggRARlWSEPQKPU7pGCKlaokNwyBUThPAF-HrhN6Hf2MH6DEMHL-N2TM5XK_fDwrMmu43LW-g8fO186_zVzHwIU7Lw82jjYHL4HQzMAX4L3lj4yfb91LjW2CfgoDN9sk_37xH48vbN5fn7avXx3cX52apq6lrmirW0wVxw3BHEiOC16BRVBtdSopZQw9ay5ZShTirMrbKEE4UVNbTlaC3Xlh6B093ecVoPtm1Kjmh6PUY3mLjVwTh9X_Huu74KG03LF2FS_K_2_hh-TjZlPbjUlBjG25JTCypkjWr8XxDXlGNJWAFPdmATQ0rRdnfHYKTn0vRcmhZynufSiuPF3xnu-H1LRX-512fjrXpvwfE_Ad1NfZ_tr1zI5zvyOuUQ_1yGEcP0Bt6ts_4</recordid><startdate>19811001</startdate><enddate>19811001</enddate><creator>Saling, Patricia M.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19811001</creationdate><title>Involvement of Trypsin-Like Activity in Binding of Mouse Spermatozoa to Zonae Pellucidae</title><author>Saling, Patricia M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c558t-4d3c16761f20427657f939a15880d23a4b8d6340f8916e9e2629193a3d60b8be3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1981</creationdate><topic>Animals</topic><topic>Cell membranes</topic><topic>Chemical suspensions</topic><topic>Female</topic><topic>Fertilization</topic><topic>Fertilization in Vitro</topic><topic>Gametes</topic><topic>Herpes zoster</topic><topic>Male</topic><topic>Mice</topic><topic>Ova</topic><topic>Ovum - physiology</topic><topic>Protease inhibitors</topic><topic>Protease Inhibitors - pharmacology</topic><topic>Sperm-Ovum Interactions - drug effects</topic><topic>Spermatozoa</topic><topic>Spermatozoa - drug effects</topic><topic>Spermatozoa - physiology</topic><topic>Trypsin - metabolism</topic><topic>Trypsin inhibitors</topic><topic>trysin</topic><topic>Zona pellucida</topic><topic>Zona Pellucida - drug effects</topic><topic>Zona Pellucida - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Saling, Patricia M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saling, Patricia M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Involvement of Trypsin-Like Activity in Binding of Mouse Spermatozoa to Zonae Pellucidae</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1981-10-01</date><risdate>1981</risdate><volume>78</volume><issue>10</issue><spage>6231</spage><epage>6235</epage><pages>6231-6235</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Work from a number of laboratories has shown that fertilization is blocked in the presence of protease inhibitors, although the specific site of inhibition has not been identified. The present experiments were designed to discriminate between sperm binding to zonae pellucidae as opposed to sperm penetration through zonae, so as to assess the effect of protease inhibitors on these two distinct events. Exposure of capacitated mouse spermatozoa to a variety of protease inhibitors directed against trypsin blocked sperm binding to zonae in a concentration-dependent manner. A chymotrypsin-directed inhibitor was not capable of blocking sperm binding to zonae. The trypsin inhibitors did not affect sperm penetration through zonae nor gamete membrane fusion if the sperm had established a firm association with the zona surface before addition of the inhibitors. Previous incubation of zona-intact eggs with the inhibitors did not lead to a reduction in sperm binding, indicating that the activity affected by the inhibitors is borne by spermatozoa. Interaction between spermatozoa and the zona surface appeared to be the specific locus of inhibition; sperm binding to zona-free eggs (i.e., binding to the egg plasma membrane) was unaltered by the trypsin inhibitors. These results suggest a reevaluation of the function of proteases in fertilization focusing on their role in initial sperm contact with the zona pellucida.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>6947226</pmid><doi>10.1073/pnas.78.10.6231</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0027-8424
ispartof Proceedings of the National Academy of Sciences - PNAS, 1981-10, Vol.78 (10), p.6231-6235
issn 0027-8424
1091-6490
language eng
recordid cdi_pnas_primary_78_10_6231
source MEDLINE; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry; JSTOR
subjects Animals
Cell membranes
Chemical suspensions
Female
Fertilization
Fertilization in Vitro
Gametes
Herpes zoster
Male
Mice
Ova
Ovum - physiology
Protease inhibitors
Protease Inhibitors - pharmacology
Sperm-Ovum Interactions - drug effects
Spermatozoa
Spermatozoa - drug effects
Spermatozoa - physiology
Trypsin - metabolism
Trypsin inhibitors
trysin
Zona pellucida
Zona Pellucida - drug effects
Zona Pellucida - physiology
title Involvement of Trypsin-Like Activity in Binding of Mouse Spermatozoa to Zonae Pellucidae
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T05%3A57%3A46IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_pnas_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Involvement%20of%20Trypsin-Like%20Activity%20in%20Binding%20of%20Mouse%20Spermatozoa%20to%20Zonae%20Pellucidae&rft.jtitle=Proceedings%20of%20the%20National%20Academy%20of%20Sciences%20-%20PNAS&rft.au=Saling,%20Patricia%20M.&rft.date=1981-10-01&rft.volume=78&rft.issue=10&rft.spage=6231&rft.epage=6235&rft.pages=6231-6235&rft.issn=0027-8424&rft.eissn=1091-6490&rft_id=info:doi/10.1073/pnas.78.10.6231&rft_dat=%3Cjstor_pnas_%3E11041%3C/jstor_pnas_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15361824&rft_id=info:pmid/6947226&rft_jstor_id=11041&rfr_iscdi=true