A Human Soluble Suppressor Factor Affecting Lymphocyte Responses in vitro
A soluble suppressor factor (SSF) has been demonstrated in the supernatant of normal human peripheral blood lymphocyte cultures that exhibits suppressive activity toward the proliferative response of normal lymphocytes to concanavalin A or alloantigens in mixed lymphocyte culture (MLC) or toward pok...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1980-10, Vol.77 (10), p.6096-6100 |
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| container_issue | 10 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Shou, Lien Schwartz, Stanley A. Good, Robert A. Peng, Reiling Chen, Cheng Lung |
| description | A soluble suppressor factor (SSF) has been demonstrated in the supernatant of normal human peripheral blood lymphocyte cultures that exhibits suppressive activity toward the proliferative response of normal lymphocytes to concanavalin A or alloantigens in mixed lymphocyte culture (MLC) or toward pokeweed mitogen-stimulated immunoglobulin synthesis and secretion in vitro. Suppression of the proliferative response in MLC reached maximal levels when added SSF-containing supernatant approximated 20% by volume of the culture medium. Suppression in the MLC was found to act at the proliferative stage. SSF acts independently of cytotoxicity and is stable at 56 degrees C for 30 min but is inactivated at higher temperatures. Addition of SSF to the MLC as late as day 4 after initiation of the culture results in suppression of transformation. This factor(s) may regulate the magnitude of several immune responses in humans. |
| doi_str_mv | 10.1073/pnas.77.10.6096 |
| format | Article |
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Suppression of the proliferative response in MLC reached maximal levels when added SSF-containing supernatant approximated 20% by volume of the culture medium. Suppression in the MLC was found to act at the proliferative stage. SSF acts independently of cytotoxicity and is stable at 56 degrees C for 30 min but is inactivated at higher temperatures. Addition of SSF to the MLC as late as day 4 after initiation of the culture results in suppression of transformation. 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Suppression of the proliferative response in MLC reached maximal levels when added SSF-containing supernatant approximated 20% by volume of the culture medium. Suppression in the MLC was found to act at the proliferative stage. SSF acts independently of cytotoxicity and is stable at 56 degrees C for 30 min but is inactivated at higher temperatures. Addition of SSF to the MLC as late as day 4 after initiation of the culture results in suppression of transformation. This factor(s) may regulate the magnitude of several immune responses in humans.</description><subject>Antibody Formation - drug effects</subject><subject>B lymphocytes</subject><subject>Blood</subject><subject>Cells, Cultured</subject><subject>Cultured cells</subject><subject>Cytotoxicity, Immunologic - drug effects</subject><subject>Humans</subject><subject>Immune Tolerance</subject><subject>Immunology</subject><subject>Lymphocyte activation</subject><subject>Lymphocyte Activation - drug effects</subject><subject>Lymphocyte Culture Test, Mixed</subject><subject>Lymphocytes</subject><subject>Lymphocytes - immunology</subject><subject>Lymphokines - pharmacology</subject><subject>Mitogens</subject><subject>Mixed lymphocyte culture test</subject><subject>Plasma cells</subject><subject>T lymphocytes</subject><subject>T-Lymphocytes, Regulatory - immunology</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kctLxDAQxoMouj7O4kHpSU9dJ22aNAcPi_iCBcHHOaTtVCttU5N2cf97U3Zd9OJlhuH7fZPwDSHHFKYURHzZtdpNhfDDlIPkW2RCQdKQMwnbZAIQiTBlEdsj-859AIBMUtglu5wxKSCakIdZcD80ug2eTT1kNQbPQ9dZdM7Y4FbnvW-zssS8r9q3YL5suneTL3sMntB1pnXogqoNFlVvzSHZKXXt8GjdD8jr7c3L9X04f7x7uJ7NwzwWCQ9LqXkhaSa5Ro55xjiAFkXKIUGqkSWy8CWLhUhoUqQpCgGS6TjVOsOI8viAXK32dkPWYJFj21tdq85WjbZLZXSl_ipt9a7ezELFCUQReP_52m_N54CuV03lcqxr3aIZnBJJzGTEUw9ersDcGucslps3KKgxfDWGr4QY5zF87zj9_bUNv07b62drfTT-qH8WXPwLqHKo6x6_ek-erMgP52-0QSUTIv4Gh7WiYQ</recordid><startdate>19801001</startdate><enddate>19801001</enddate><creator>Shou, Lien</creator><creator>Schwartz, Stanley A.</creator><creator>Good, Robert A.</creator><creator>Peng, Reiling</creator><creator>Chen, Cheng Lung</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19801001</creationdate><title>A Human Soluble Suppressor Factor Affecting Lymphocyte Responses in vitro</title><author>Shou, Lien ; Schwartz, Stanley A. ; Good, Robert A. ; Peng, Reiling ; Chen, Cheng Lung</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3756-f9a6d91b96ae6ecb4600a7d8605e1ae459de45b377515d88e77094a38aabe2163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><topic>Antibody Formation - drug effects</topic><topic>B lymphocytes</topic><topic>Blood</topic><topic>Cells, Cultured</topic><topic>Cultured cells</topic><topic>Cytotoxicity, Immunologic - drug effects</topic><topic>Humans</topic><topic>Immune Tolerance</topic><topic>Immunology</topic><topic>Lymphocyte activation</topic><topic>Lymphocyte Activation - drug effects</topic><topic>Lymphocyte Culture Test, Mixed</topic><topic>Lymphocytes</topic><topic>Lymphocytes - immunology</topic><topic>Lymphokines - pharmacology</topic><topic>Mitogens</topic><topic>Mixed lymphocyte culture test</topic><topic>Plasma cells</topic><topic>T lymphocytes</topic><topic>T-Lymphocytes, Regulatory - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shou, Lien</creatorcontrib><creatorcontrib>Schwartz, Stanley A.</creatorcontrib><creatorcontrib>Good, Robert A.</creatorcontrib><creatorcontrib>Peng, Reiling</creatorcontrib><creatorcontrib>Chen, Cheng Lung</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shou, Lien</au><au>Schwartz, Stanley A.</au><au>Good, Robert A.</au><au>Peng, Reiling</au><au>Chen, Cheng Lung</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Human Soluble Suppressor Factor Affecting Lymphocyte Responses in vitro</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1980-10-01</date><risdate>1980</risdate><volume>77</volume><issue>10</issue><spage>6096</spage><epage>6100</epage><pages>6096-6100</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>A soluble suppressor factor (SSF) has been demonstrated in the supernatant of normal human peripheral blood lymphocyte cultures that exhibits suppressive activity toward the proliferative response of normal lymphocytes to concanavalin A or alloantigens in mixed lymphocyte culture (MLC) or toward pokeweed mitogen-stimulated immunoglobulin synthesis and secretion in vitro. Suppression of the proliferative response in MLC reached maximal levels when added SSF-containing supernatant approximated 20% by volume of the culture medium. Suppression in the MLC was found to act at the proliferative stage. SSF acts independently of cytotoxicity and is stable at 56 degrees C for 30 min but is inactivated at higher temperatures. Addition of SSF to the MLC as late as day 4 after initiation of the culture results in suppression of transformation. This factor(s) may regulate the magnitude of several immune responses in humans.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>6449702</pmid><doi>10.1073/pnas.77.10.6096</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1980-10, Vol.77 (10), p.6096-6100 |
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| subjects | Antibody Formation - drug effects B lymphocytes Blood Cells, Cultured Cultured cells Cytotoxicity, Immunologic - drug effects Humans Immune Tolerance Immunology Lymphocyte activation Lymphocyte Activation - drug effects Lymphocyte Culture Test, Mixed Lymphocytes Lymphocytes - immunology Lymphokines - pharmacology Mitogens Mixed lymphocyte culture test Plasma cells T lymphocytes T-Lymphocytes, Regulatory - immunology |
| title | A Human Soluble Suppressor Factor Affecting Lymphocyte Responses in vitro |
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