Growth of a Rat Neuroblastoma Cell Line in Serum-Free Supplemented Medium
The rat neuroblastoma B104 cell line, which originated in the central nervous system, was able to proliferate in the absence of serum in synthetic medium supplemented with insulin, transferrin, progesterone, selenium, and putrescine. When added individually, each supplement had little or no effect;...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1979-01, Vol.76 (1), p.514-517 |
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| container_end_page | 517 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 76 |
| creator | Bottenstein, Jane E. Sato, Gordon H. |
| description | The rat neuroblastoma B104 cell line, which originated in the central nervous system, was able to proliferate in the absence of serum in synthetic medium supplemented with insulin, transferrin, progesterone, selenium, and putrescine. When added individually, each supplement had little or no effect; however, in combination there was a marked synergistic effect on cell number. The cells attained the same saturation density in this medium as in medium with 10% fetal calf serum added. More extensive process formation was observed in the supplemented medium, and other differentiated properties were retained as well. |
| doi_str_mv | 10.1073/pnas.76.1.514 |
| format | Article |
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When added individually, each supplement had little or no effect; however, in combination there was a marked synergistic effect on cell number. The cells attained the same saturation density in this medium as in medium with 10% fetal calf serum added. More extensive process formation was observed in the supplemented medium, and other differentiated properties were retained as well.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.76.1.514</identifier><identifier>PMID: 284369</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Animals ; Blood ; Cell Division - drug effects ; Cell growth ; Cell Line ; Cell lines ; Central nervous system ; Culture Media ; Cultured cells ; Hormones ; Hormones - pharmacology ; Insulin ; Insulin - pharmacology ; Neuroblastoma ; Neuroblastoma - pathology ; Neurons ; Putrescine - pharmacology ; Rats ; Selenium ; Selenium - pharmacology ; Transferrin - pharmacology ; Transferrins</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1979-01, Vol.76 (1), p.514-517</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c516t-a68183e9fc82c5691c447d76a7d78481da3b45d399805323c3c09d6f869935bc3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/76/1.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/69528$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/69528$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27915,27916,53782,53784,58008,58241</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/284369$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bottenstein, Jane E.</creatorcontrib><creatorcontrib>Sato, Gordon H.</creatorcontrib><title>Growth of a Rat Neuroblastoma Cell Line in Serum-Free Supplemented Medium</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The rat neuroblastoma B104 cell line, which originated in the central nervous system, was able to proliferate in the absence of serum in synthetic medium supplemented with insulin, transferrin, progesterone, selenium, and putrescine. When added individually, each supplement had little or no effect; however, in combination there was a marked synergistic effect on cell number. The cells attained the same saturation density in this medium as in medium with 10% fetal calf serum added. More extensive process formation was observed in the supplemented medium, and other differentiated properties were retained as well.</description><subject>Animals</subject><subject>Blood</subject><subject>Cell Division - drug effects</subject><subject>Cell growth</subject><subject>Cell Line</subject><subject>Cell lines</subject><subject>Central nervous system</subject><subject>Culture Media</subject><subject>Cultured cells</subject><subject>Hormones</subject><subject>Hormones - pharmacology</subject><subject>Insulin</subject><subject>Insulin - pharmacology</subject><subject>Neuroblastoma</subject><subject>Neuroblastoma - pathology</subject><subject>Neurons</subject><subject>Putrescine - pharmacology</subject><subject>Rats</subject><subject>Selenium</subject><subject>Selenium - pharmacology</subject><subject>Transferrin - pharmacology</subject><subject>Transferrins</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1979</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkUtP3DAUhS3Ea5iyZINYeNPuMrXjR-xFF2hUKNJAJYauLY9zA0FJnNoJbf99PZphBBIbe_Gdc33uMUJnlMwoKdjXvrNxVsgZnQnK99CEEk0zyTXZRxNC8iJTPOfH6CTGZ0KIFoococNccSb1BN1cB_9neMK-whbf2wHfwRj8qrFx8K3Fc2gavKg7wHWHlxDGNrsKAHg59n0DLXQDlPgWynpsP6GDyjYRTrf3FP26-v4w_5Etfl7fzC8XmRNUDpmViioGunIqd0Jq6jgvykLadCiuaGnZiouSaa2IYDlzzBFdykpJrZlYOTZF3zZz-3HVQulShmAb04e6teGf8bY270lXP5lH_2KYynWRJ_-XrT_43yPEwbR1dGlP24EfoylSHiqkSMJsI3TBxxig2r1BiVk3b9bNm0IaalLzSX_xNthOvak64c9bvHa9wle3qcamGeDv8GbMR7qEzzf4Of1Q2HGpRa7Yf5Ivnv0</recordid><startdate>19790101</startdate><enddate>19790101</enddate><creator>Bottenstein, Jane E.</creator><creator>Sato, Gordon H.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19790101</creationdate><title>Growth of a Rat Neuroblastoma Cell Line in Serum-Free Supplemented Medium</title><author>Bottenstein, Jane E. ; Sato, Gordon H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c516t-a68183e9fc82c5691c447d76a7d78481da3b45d399805323c3c09d6f869935bc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1979</creationdate><topic>Animals</topic><topic>Blood</topic><topic>Cell Division - drug effects</topic><topic>Cell growth</topic><topic>Cell Line</topic><topic>Cell lines</topic><topic>Central nervous system</topic><topic>Culture Media</topic><topic>Cultured cells</topic><topic>Hormones</topic><topic>Hormones - pharmacology</topic><topic>Insulin</topic><topic>Insulin - pharmacology</topic><topic>Neuroblastoma</topic><topic>Neuroblastoma - pathology</topic><topic>Neurons</topic><topic>Putrescine - pharmacology</topic><topic>Rats</topic><topic>Selenium</topic><topic>Selenium - pharmacology</topic><topic>Transferrin - pharmacology</topic><topic>Transferrins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bottenstein, Jane E.</creatorcontrib><creatorcontrib>Sato, Gordon H.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bottenstein, Jane E.</au><au>Sato, Gordon H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Growth of a Rat Neuroblastoma Cell Line in Serum-Free Supplemented Medium</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1979-01-01</date><risdate>1979</risdate><volume>76</volume><issue>1</issue><spage>514</spage><epage>517</epage><pages>514-517</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>The rat neuroblastoma B104 cell line, which originated in the central nervous system, was able to proliferate in the absence of serum in synthetic medium supplemented with insulin, transferrin, progesterone, selenium, and putrescine. When added individually, each supplement had little or no effect; however, in combination there was a marked synergistic effect on cell number. The cells attained the same saturation density in this medium as in medium with 10% fetal calf serum added. More extensive process formation was observed in the supplemented medium, and other differentiated properties were retained as well.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>284369</pmid><doi>10.1073/pnas.76.1.514</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1979-01, Vol.76 (1), p.514-517 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_pnas_primary_76_1_514_fulltext |
| source | MEDLINE; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry; JSTOR |
| subjects | Animals Blood Cell Division - drug effects Cell growth Cell Line Cell lines Central nervous system Culture Media Cultured cells Hormones Hormones - pharmacology Insulin Insulin - pharmacology Neuroblastoma Neuroblastoma - pathology Neurons Putrescine - pharmacology Rats Selenium Selenium - pharmacology Transferrin - pharmacology Transferrins |
| title | Growth of a Rat Neuroblastoma Cell Line in Serum-Free Supplemented Medium |
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