Polyamine-Pyridoxal Schiff Bases in Urine
Schiff bases of the diamines 1,3-diaminopropane, putrescine, and cadaverine and the polyamines spermidine and spermine with pyridoxal or pyridoxal phosphate occur in human urine, as shown by gas chromatographic/mass spectrometric selected ion-monitoring techniques. By use of synthetic standards, pro...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1979-12, Vol.76 (12), p.6652-6655 |
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| container_issue | 12 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Aigner-Held, Rosemarie Campbell, Robert A. Daves, G. Doyle |
| description | Schiff bases of the diamines 1,3-diaminopropane, putrescine, and cadaverine and the polyamines spermidine and spermine with pyridoxal or pyridoxal phosphate occur in human urine, as shown by gas chromatographic/mass spectrometric selected ion-monitoring techniques. By use of synthetic standards, procedures were devised for conversion of the Schiff bases to stable derivatives amenable to gas chromatographic/mass spectrometric analysis. These procedures involve borohydride reduction of the [Note: See the image of page 6652 for this formatted text] C==N double bond, hydrolytic removal of the phosphate group, chromatographic separation from the bulk of urinary constituents, and trifluoroacetylation of polar functional groups. The levels of the polyamine-pyridoxal Schiff bases were estimated to be in the range of pmol/ml of urine. |
| doi_str_mv | 10.1073/pnas.76.12.6652 |
| format | Article |
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Doyle</creatorcontrib><title>Polyamine-Pyridoxal Schiff Bases in Urine</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Schiff bases of the diamines 1,3-diaminopropane, putrescine, and cadaverine and the polyamines spermidine and spermine with pyridoxal or pyridoxal phosphate occur in human urine, as shown by gas chromatographic/mass spectrometric selected ion-monitoring techniques. By use of synthetic standards, procedures were devised for conversion of the Schiff bases to stable derivatives amenable to gas chromatographic/mass spectrometric analysis. These procedures involve borohydride reduction of the [Note: See the image of page 6652 for this formatted text] C==N double bond, hydrolytic removal of the phosphate group, chromatographic separation from the bulk of urinary constituents, and trifluoroacetylation of polar functional groups. The levels of the polyamine-pyridoxal Schiff bases were estimated to be in the range of pmol/ml of urine.</description><subject>Adult</subject><subject>Biochemistry</subject><subject>Body fluids</subject><subject>Borohydrides</subject><subject>Cadaverine - urine</subject><subject>Chemical Phenomena</subject><subject>Chemistry</subject><subject>Gas Chromatography-Mass Spectrometry</subject><subject>Humans</subject><subject>Ions</subject><subject>Isomerism</subject><subject>Mass spectrometers</subject><subject>Phosphates</subject><subject>Polyamines</subject><subject>Polyamines - urine</subject><subject>Putrescine - urine</subject><subject>Pyridoxal - urine</subject><subject>Pyridoxal Phosphate - urine</subject><subject>Schiff bases</subject><subject>Schiff Bases - urine</subject><subject>Sodium</subject><subject>Spermidine - urine</subject><subject>Spermine - urine</subject><subject>Urine</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1979</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM1LwzAYh4P4NadnQUR6Ujy0e_PRpDl40OEXDBzoziFrU1fp2pm0sv33tnRuevGUw_M8SfghdIohwCDoYFFoFwgeYBJwHpId1MMgsc-ZhF3UAyDCjxhhh-jIuQ8AkGEEB2ifSCpC6KHrcZmv9DwrjD9e2Swplzr3XuNZlqbenXbGeVnhTWzDj9FeqnNnTtZnH00e7t-GT_7o5fF5eDvyY8ah8okUIcOGh9qIUAgRaxzKiJCES0pTQkOeGC5kMtUmSTUBpg2FSBqGeUSooLSPbrp7F_V0bpLYFJXVuVrYbK7tSpU6U39Jkc3Ue_mlGMaS8Ka_XPe2_KyNq9Q8c7HJc12YsnZKsIhRgqERB50Y29I5a9LNGxhUu61qt1WCK0xUu21TnP_-2sbvxmzw1Rq33Q_c9iqt87wyy6oxL_41G-GsEz5cVdqtARQI_QYtCJUb</recordid><startdate>19791201</startdate><enddate>19791201</enddate><creator>Aigner-Held, Rosemarie</creator><creator>Campbell, Robert A.</creator><creator>Daves, G. 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Doyle</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c460t-297541e65ae75777ca159822d6933f2356de679dbaedfa204ae3089e416823733</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1979</creationdate><topic>Adult</topic><topic>Biochemistry</topic><topic>Body fluids</topic><topic>Borohydrides</topic><topic>Cadaverine - urine</topic><topic>Chemical Phenomena</topic><topic>Chemistry</topic><topic>Gas Chromatography-Mass Spectrometry</topic><topic>Humans</topic><topic>Ions</topic><topic>Isomerism</topic><topic>Mass spectrometers</topic><topic>Phosphates</topic><topic>Polyamines</topic><topic>Polyamines - urine</topic><topic>Putrescine - urine</topic><topic>Pyridoxal - urine</topic><topic>Pyridoxal Phosphate - urine</topic><topic>Schiff bases</topic><topic>Schiff Bases - urine</topic><topic>Sodium</topic><topic>Spermidine - urine</topic><topic>Spermine - urine</topic><topic>Urine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Aigner-Held, Rosemarie</creatorcontrib><creatorcontrib>Campbell, Robert A.</creatorcontrib><creatorcontrib>Daves, G. 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Doyle</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Polyamine-Pyridoxal Schiff Bases in Urine</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1979-12-01</date><risdate>1979</risdate><volume>76</volume><issue>12</issue><spage>6652</spage><epage>6655</epage><pages>6652-6655</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Schiff bases of the diamines 1,3-diaminopropane, putrescine, and cadaverine and the polyamines spermidine and spermine with pyridoxal or pyridoxal phosphate occur in human urine, as shown by gas chromatographic/mass spectrometric selected ion-monitoring techniques. By use of synthetic standards, procedures were devised for conversion of the Schiff bases to stable derivatives amenable to gas chromatographic/mass spectrometric analysis. These procedures involve borohydride reduction of the [Note: See the image of page 6652 for this formatted text] C==N double bond, hydrolytic removal of the phosphate group, chromatographic separation from the bulk of urinary constituents, and trifluoroacetylation of polar functional groups. The levels of the polyamine-pyridoxal Schiff bases were estimated to be in the range of pmol/ml of urine.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>293750</pmid><doi>10.1073/pnas.76.12.6652</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1979-12, Vol.76 (12), p.6652-6655 |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Adult Biochemistry Body fluids Borohydrides Cadaverine - urine Chemical Phenomena Chemistry Gas Chromatography-Mass Spectrometry Humans Ions Isomerism Mass spectrometers Phosphates Polyamines Polyamines - urine Putrescine - urine Pyridoxal - urine Pyridoxal Phosphate - urine Schiff bases Schiff Bases - urine Sodium Spermidine - urine Spermine - urine Urine |
| title | Polyamine-Pyridoxal Schiff Bases in Urine |
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