Purification and Translation of Zein Messenger RNA from Maize Endosperm Protein Bodies
The messenger RNAs coding for the zein storage protein have been purified from other contaminating RNAs. The average molecular lengths are 1.1-1.2 kilobases, as determined by polyacrylamide gel electrophoresis and by electron microscopy. Products of messenger-dependent protein synthesis in vitro app...
Gespeichert in:
| Veröffentlicht in: | Proc. Natl. Acad. Sci. U.S.A.; (United States) 1978-02, Vol.75 (2), p.696-700 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 700 |
|---|---|
| container_issue | 2 |
| container_start_page | 696 |
| container_title | Proc. Natl. Acad. Sci. U.S.A.; (United States) |
| container_volume | 75 |
| creator | Burr, B. Burr, F. A. Rubenstein, I. Simon, M. N. |
| description | The messenger RNAs coding for the zein storage protein have been purified from other contaminating RNAs. The average molecular lengths are 1.1-1.2 kilobases, as determined by polyacrylamide gel electrophoresis and by electron microscopy. Products of messenger-dependent protein synthesis in vitro appear to be 1100 and 2000 daltons heavier than the native polypeptides. Zein is like secretory proteins in having a precursor with an additional amino-terminal sequence. Although only one mRNA is seen in polyacrylamide gel electrophoresis, the combined size of the polypeptide products formed exceeds the coding capacity for one message of the size determined in this study. This suggests that there are at least two mRNAs of similar sizes for the zein polypeptides rather than one dicistronic message. |
| doi_str_mv | 10.1073/pnas.75.2.696 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_osti_</sourceid><recordid>TN_cdi_pnas_primary_75_2_696_fulltext</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>67853</jstor_id><sourcerecordid>67853</sourcerecordid><originalsourceid>FETCH-LOGICAL-c480t-abfcd48129f7c4e87e466064c24e51d4e8e2408ffbeee887279710578c5f11843</originalsourceid><addsrcrecordid>eNp9kc1vFCEYxonR2LV69GKMmZgYT7MCwwBz6KE2rZq02pjqwQthmZeWZgZWYJrav75sZlvrxROB5_fwfjwIvSR4SbBoPqy9TkvRLumSd_wRWhDckZqzDj9GC4ypqCWjbAc9S-kSY9y1Ej9FO4S3HWUdX6Cfp1N01hmdXfCV9n11FrVPw3wPtvoFzlcnkBL4c4jV96_7lY1hrE60u4Hq0PchrSGO1WkMeYN-DL2D9Bw9sXpI8GJ77qIfR4dnB5_r42-fvhzsH9eGSZxrvbKmZ5LQzgrDQApgnGPODGXQkr68AGVYWrsCACkFFZ0guBXStJYQyZpdtDf_u55WI_QGfI56UOvoRh3_qKCd-lfx7kKdhyvFCGloU_xvZ39I2alkXAZzYYL3YLLiXMhSrkDvt0Vi-D1Bymp0ycAwaA9hSko0Tdllx3gh65k0MaQUwd53QrDaxKU2cSnRKqpKXIV_87D9v_Q2nwK83gIb3538wP_uP7Ky0zBkuM6FezVzlymHeA-W-dqmuQUV67Gk</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>733496946</pqid></control><display><type>article</type><title>Purification and Translation of Zein Messenger RNA from Maize Endosperm Protein Bodies</title><source>Jstor Complete Legacy</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>Burr, B. ; Burr, F. A. ; Rubenstein, I. ; Simon, M. N.</creator><creatorcontrib>Burr, B. ; Burr, F. A. ; Rubenstein, I. ; Simon, M. N. ; Brookhaven National Lab., Upton, NY</creatorcontrib><description>The messenger RNAs coding for the zein storage protein have been purified from other contaminating RNAs. The average molecular lengths are 1.1-1.2 kilobases, as determined by polyacrylamide gel electrophoresis and by electron microscopy. Products of messenger-dependent protein synthesis in vitro appear to be 1100 and 2000 daltons heavier than the native polypeptides. Zein is like secretory proteins in having a precursor with an additional amino-terminal sequence. Although only one mRNA is seen in polyacrylamide gel electrophoresis, the combined size of the polypeptide products formed exceeds the coding capacity for one message of the size determined in this study. This suggests that there are at least two mRNAs of similar sizes for the zein polypeptides rather than one dicistronic message.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.75.2.696</identifier><identifier>PMID: 16592496</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>550200 -- Biochemistry ; 550201 -- Biochemistry-- Tracer Techniques ; 550401 -- Genetics-- Tracer Techniques ; Amino acids ; BASIC BIOLOGICAL SCIENCES ; BIOCHEMISTRY ; Biological Sciences: Biochemistry ; BIOLOGY ; BIOMASS ; Bromides ; CEREALS ; CHEMISTRY ; Corn ; ELECTRON MICROSCOPY ; ELECTROPHORESIS ; ENERGY SOURCES ; Gels ; GENETICS ; GRAMINEAE ; GRASS ; LABELLED COMPOUNDS ; MAIZE ; MESSENGER-RNA ; MICROSCOPY ; MOLECULAR BIOLOGY ; MOLECULAR STRUCTURE ; Molecular weight ; NUCLEIC ACIDS ; ORGANIC COMPOUNDS ; PLANTS ; Polyribosomes ; PROTEINS ; PURIFICATION ; RENEWABLE ENERGY SOURCES ; RNA ; RNA 550400 -- Genetics ; SYNTHESIS</subject><ispartof>Proc. Natl. Acad. Sci. U.S.A.; (United States), 1978-02, Vol.75 (2), p.696-700</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c480t-abfcd48129f7c4e87e466064c24e51d4e8e2408ffbeee887279710578c5f11843</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/75/2.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/67853$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/67853$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,724,777,781,800,882,27905,27906,53772,53774,57998,58231</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16592496$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/6678710$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Burr, B.</creatorcontrib><creatorcontrib>Burr, F. A.</creatorcontrib><creatorcontrib>Rubenstein, I.</creatorcontrib><creatorcontrib>Simon, M. N.</creatorcontrib><creatorcontrib>Brookhaven National Lab., Upton, NY</creatorcontrib><title>Purification and Translation of Zein Messenger RNA from Maize Endosperm Protein Bodies</title><title>Proc. Natl. Acad. Sci. U.S.A.; (United States)</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The messenger RNAs coding for the zein storage protein have been purified from other contaminating RNAs. The average molecular lengths are 1.1-1.2 kilobases, as determined by polyacrylamide gel electrophoresis and by electron microscopy. Products of messenger-dependent protein synthesis in vitro appear to be 1100 and 2000 daltons heavier than the native polypeptides. Zein is like secretory proteins in having a precursor with an additional amino-terminal sequence. Although only one mRNA is seen in polyacrylamide gel electrophoresis, the combined size of the polypeptide products formed exceeds the coding capacity for one message of the size determined in this study. This suggests that there are at least two mRNAs of similar sizes for the zein polypeptides rather than one dicistronic message.</description><subject>550200 -- Biochemistry</subject><subject>550201 -- Biochemistry-- Tracer Techniques</subject><subject>550401 -- Genetics-- Tracer Techniques</subject><subject>Amino acids</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>BIOCHEMISTRY</subject><subject>Biological Sciences: Biochemistry</subject><subject>BIOLOGY</subject><subject>BIOMASS</subject><subject>Bromides</subject><subject>CEREALS</subject><subject>CHEMISTRY</subject><subject>Corn</subject><subject>ELECTRON MICROSCOPY</subject><subject>ELECTROPHORESIS</subject><subject>ENERGY SOURCES</subject><subject>Gels</subject><subject>GENETICS</subject><subject>GRAMINEAE</subject><subject>GRASS</subject><subject>LABELLED COMPOUNDS</subject><subject>MAIZE</subject><subject>MESSENGER-RNA</subject><subject>MICROSCOPY</subject><subject>MOLECULAR BIOLOGY</subject><subject>MOLECULAR STRUCTURE</subject><subject>Molecular weight</subject><subject>NUCLEIC ACIDS</subject><subject>ORGANIC COMPOUNDS</subject><subject>PLANTS</subject><subject>Polyribosomes</subject><subject>PROTEINS</subject><subject>PURIFICATION</subject><subject>RENEWABLE ENERGY SOURCES</subject><subject>RNA</subject><subject>RNA 550400 -- Genetics</subject><subject>SYNTHESIS</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1978</creationdate><recordtype>article</recordtype><recordid>eNp9kc1vFCEYxonR2LV69GKMmZgYT7MCwwBz6KE2rZq02pjqwQthmZeWZgZWYJrav75sZlvrxROB5_fwfjwIvSR4SbBoPqy9TkvRLumSd_wRWhDckZqzDj9GC4ypqCWjbAc9S-kSY9y1Ej9FO4S3HWUdX6Cfp1N01hmdXfCV9n11FrVPw3wPtvoFzlcnkBL4c4jV96_7lY1hrE60u4Hq0PchrSGO1WkMeYN-DL2D9Bw9sXpI8GJ77qIfR4dnB5_r42-fvhzsH9eGSZxrvbKmZ5LQzgrDQApgnGPODGXQkr68AGVYWrsCACkFFZ0guBXStJYQyZpdtDf_u55WI_QGfI56UOvoRh3_qKCd-lfx7kKdhyvFCGloU_xvZ39I2alkXAZzYYL3YLLiXMhSrkDvt0Vi-D1Bymp0ycAwaA9hSko0Tdllx3gh65k0MaQUwd53QrDaxKU2cSnRKqpKXIV_87D9v_Q2nwK83gIb3538wP_uP7Ky0zBkuM6FezVzlymHeA-W-dqmuQUV67Gk</recordid><startdate>19780201</startdate><enddate>19780201</enddate><creator>Burr, B.</creator><creator>Burr, F. A.</creator><creator>Rubenstein, I.</creator><creator>Simon, M. N.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope><scope>5PM</scope></search><sort><creationdate>19780201</creationdate><title>Purification and Translation of Zein Messenger RNA from Maize Endosperm Protein Bodies</title><author>Burr, B. ; Burr, F. A. ; Rubenstein, I. ; Simon, M. N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c480t-abfcd48129f7c4e87e466064c24e51d4e8e2408ffbeee887279710578c5f11843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1978</creationdate><topic>550200 -- Biochemistry</topic><topic>550201 -- Biochemistry-- Tracer Techniques</topic><topic>550401 -- Genetics-- Tracer Techniques</topic><topic>Amino acids</topic><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>BIOCHEMISTRY</topic><topic>Biological Sciences: Biochemistry</topic><topic>BIOLOGY</topic><topic>BIOMASS</topic><topic>Bromides</topic><topic>CEREALS</topic><topic>CHEMISTRY</topic><topic>Corn</topic><topic>ELECTRON MICROSCOPY</topic><topic>ELECTROPHORESIS</topic><topic>ENERGY SOURCES</topic><topic>Gels</topic><topic>GENETICS</topic><topic>GRAMINEAE</topic><topic>GRASS</topic><topic>LABELLED COMPOUNDS</topic><topic>MAIZE</topic><topic>MESSENGER-RNA</topic><topic>MICROSCOPY</topic><topic>MOLECULAR BIOLOGY</topic><topic>MOLECULAR STRUCTURE</topic><topic>Molecular weight</topic><topic>NUCLEIC ACIDS</topic><topic>ORGANIC COMPOUNDS</topic><topic>PLANTS</topic><topic>Polyribosomes</topic><topic>PROTEINS</topic><topic>PURIFICATION</topic><topic>RENEWABLE ENERGY SOURCES</topic><topic>RNA</topic><topic>RNA 550400 -- Genetics</topic><topic>SYNTHESIS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Burr, B.</creatorcontrib><creatorcontrib>Burr, F. A.</creatorcontrib><creatorcontrib>Rubenstein, I.</creatorcontrib><creatorcontrib>Simon, M. N.</creatorcontrib><creatorcontrib>Brookhaven National Lab., Upton, NY</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proc. Natl. Acad. Sci. U.S.A.; (United States)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Burr, B.</au><au>Burr, F. A.</au><au>Rubenstein, I.</au><au>Simon, M. N.</au><aucorp>Brookhaven National Lab., Upton, NY</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Purification and Translation of Zein Messenger RNA from Maize Endosperm Protein Bodies</atitle><jtitle>Proc. Natl. Acad. Sci. U.S.A.; (United States)</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1978-02-01</date><risdate>1978</risdate><volume>75</volume><issue>2</issue><spage>696</spage><epage>700</epage><pages>696-700</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>The messenger RNAs coding for the zein storage protein have been purified from other contaminating RNAs. The average molecular lengths are 1.1-1.2 kilobases, as determined by polyacrylamide gel electrophoresis and by electron microscopy. Products of messenger-dependent protein synthesis in vitro appear to be 1100 and 2000 daltons heavier than the native polypeptides. Zein is like secretory proteins in having a precursor with an additional amino-terminal sequence. Although only one mRNA is seen in polyacrylamide gel electrophoresis, the combined size of the polypeptide products formed exceeds the coding capacity for one message of the size determined in this study. This suggests that there are at least two mRNAs of similar sizes for the zein polypeptides rather than one dicistronic message.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>16592496</pmid><doi>10.1073/pnas.75.2.696</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0027-8424 |
| ispartof | Proc. Natl. Acad. Sci. U.S.A.; (United States), 1978-02, Vol.75 (2), p.696-700 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_pnas_primary_75_2_696_fulltext |
| source | Jstor Complete Legacy; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | 550200 -- Biochemistry 550201 -- Biochemistry-- Tracer Techniques 550401 -- Genetics-- Tracer Techniques Amino acids BASIC BIOLOGICAL SCIENCES BIOCHEMISTRY Biological Sciences: Biochemistry BIOLOGY BIOMASS Bromides CEREALS CHEMISTRY Corn ELECTRON MICROSCOPY ELECTROPHORESIS ENERGY SOURCES Gels GENETICS GRAMINEAE GRASS LABELLED COMPOUNDS MAIZE MESSENGER-RNA MICROSCOPY MOLECULAR BIOLOGY MOLECULAR STRUCTURE Molecular weight NUCLEIC ACIDS ORGANIC COMPOUNDS PLANTS Polyribosomes PROTEINS PURIFICATION RENEWABLE ENERGY SOURCES RNA RNA 550400 -- Genetics SYNTHESIS |
| title | Purification and Translation of Zein Messenger RNA from Maize Endosperm Protein Bodies |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-17T22%3A27%3A04IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_osti_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Purification%20and%20Translation%20of%20Zein%20Messenger%20RNA%20from%20Maize%20Endosperm%20Protein%20Bodies&rft.jtitle=Proc.%20Natl.%20Acad.%20Sci.%20U.S.A.;%20(United%20States)&rft.au=Burr,%20B.&rft.aucorp=Brookhaven%20National%20Lab.,%20Upton,%20NY&rft.date=1978-02-01&rft.volume=75&rft.issue=2&rft.spage=696&rft.epage=700&rft.pages=696-700&rft.issn=0027-8424&rft.eissn=1091-6490&rft_id=info:doi/10.1073/pnas.75.2.696&rft_dat=%3Cjstor_osti_%3E67853%3C/jstor_osti_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=733496946&rft_id=info:pmid/16592496&rft_jstor_id=67853&rfr_iscdi=true |