Purification and Properties of Human Erythrocyte Pyrimidine 5′-nucleotidase

A 250,000-fold purification of pyrimidine 5′-nucleotidase from human erythrocytes has been achieved using a combination of DEAE-cellulose chromatography, ammonium sulfate fractionation, gel filtration, and isoelectric focusing. Polyacrylamide disc and starch gel electrophoreses of the purified mater...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1977-09, Vol.74 (9), p.3701-3704
Hauptverfasser:	Torrance, J. D., Whittaker, D., Beutler, E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Blood Electrophoresis Enzyme activity Enzymes Erythrocytes Erythrocytes - enzymology Gels Hemoglobins Humans Hydrogen-Ion Concentration Kinetics Nucleotidases - blood Nucleotidases - isolation & purification Pyrimidine Nucleotides Pyrimidines Quaternary ammonium compounds Starches Sulfates
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