Ribosome Structure: Localization of N6,N6-dimethyladenosine by Electron Microscopy of a Ribosome-Antibody Complex
Antibodies to the minor nucleoside N6,N6-dimethyladenosine have been used to map a unique location of the nucleoside in the small subunit of the Escherichia coli ribosome. Antibodies were induced in rabbits by a nucleoside-bovine albumin conjugate and shown to be highly specific for the dimethyladen...
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Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1977-04, Vol.74 (4), p.1468-1472 |
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description | Antibodies to the minor nucleoside N6,N6-dimethyladenosine have been used to map a unique location of the nucleoside in the small subunit of the Escherichia coli ribosome. Antibodies were induced in rabbits by a nucleoside-bovine albumin conjugate and shown to be highly specific for the dimethyladenosine hapten. The antibodies were shown to interact with 30S ribosomal subunits from strain PR7, but not with subunits from its mutant strain TPR201, which is resistant to kasugamycin and lacks the two successive residues of dimethyladenosine normally found near the 3′-end of E. coli 16S ribosomal RNA. Electron micrographs of strain PR7 subunits, crosslinked by single IgG molecules, show a single binding site on the surface of the ribosome. This binding site is consistent with observations relating the 3′-end of the ribosomal RNA, binding of initiation factor IF-3 and messenger RNA, and mapping of specific ribosomal proteins. |
doi_str_mv | 10.1073/pnas.74.4.1468 |
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Antibodies were induced in rabbits by a nucleoside-bovine albumin conjugate and shown to be highly specific for the dimethyladenosine hapten. The antibodies were shown to interact with 30S ribosomal subunits from strain PR7, but not with subunits from its mutant strain TPR201, which is resistant to kasugamycin and lacks the two successive residues of dimethyladenosine normally found near the 3′-end of E. coli 16S ribosomal RNA. Electron micrographs of strain PR7 subunits, crosslinked by single IgG molecules, show a single binding site on the surface of the ribosome. This binding site is consistent with observations relating the 3′-end of the ribosomal RNA, binding of initiation factor IF-3 and messenger RNA, and mapping of specific ribosomal proteins.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.74.4.1468</identifier><identifier>PMID: 323854</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Adenosine - analogs & derivatives ; Adenosine - immunology ; Albumins ; Antibodies ; Binding sites ; Chemical precipitation ; Electron microscopy ; Escherichia coli - ultrastructure ; Globulins ; Haptens ; Immunoassay ; Kinetics ; Microscopy, Electron ; Nucleosides ; Ribosomes ; Ribosomes - immunology ; Ribosomes - ultrastructure ; RNA ; RNA, Ribosomal - analysis ; Structure-Activity Relationship</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1977-04, Vol.74 (4), p.1468-1472</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c387t-d6ff17983014b82c761971dc1aa5e07ff7d7704b69eb37ff174588760c79c45b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/74/4.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/66724$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/66724$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/323854$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Politz, Samuel M.</creatorcontrib><creatorcontrib>Glitz, Dohn G.</creatorcontrib><title>Ribosome Structure: Localization of N6,N6-dimethyladenosine by Electron Microscopy of a Ribosome-Antibody Complex</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Antibodies to the minor nucleoside N6,N6-dimethyladenosine have been used to map a unique location of the nucleoside in the small subunit of the Escherichia coli ribosome. Antibodies were induced in rabbits by a nucleoside-bovine albumin conjugate and shown to be highly specific for the dimethyladenosine hapten. The antibodies were shown to interact with 30S ribosomal subunits from strain PR7, but not with subunits from its mutant strain TPR201, which is resistant to kasugamycin and lacks the two successive residues of dimethyladenosine normally found near the 3′-end of E. coli 16S ribosomal RNA. Electron micrographs of strain PR7 subunits, crosslinked by single IgG molecules, show a single binding site on the surface of the ribosome. This binding site is consistent with observations relating the 3′-end of the ribosomal RNA, binding of initiation factor IF-3 and messenger RNA, and mapping of specific ribosomal proteins.</description><subject>Adenosine - analogs & derivatives</subject><subject>Adenosine - immunology</subject><subject>Albumins</subject><subject>Antibodies</subject><subject>Binding sites</subject><subject>Chemical precipitation</subject><subject>Electron microscopy</subject><subject>Escherichia coli - ultrastructure</subject><subject>Globulins</subject><subject>Haptens</subject><subject>Immunoassay</subject><subject>Kinetics</subject><subject>Microscopy, Electron</subject><subject>Nucleosides</subject><subject>Ribosomes</subject><subject>Ribosomes - immunology</subject><subject>Ribosomes - ultrastructure</subject><subject>RNA</subject><subject>RNA, Ribosomal - analysis</subject><subject>Structure-Activity Relationship</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1977</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUtv1DAURi3EayhsWSCQsuqKhOvYsZ1KLKpReUhDkXisLcdxqCsnTm0HNfx6Es10VDasbOk75_rKH0IvMRQYOHk3DioWnBa0wJSJB2iDocY5ozU8RBuAkueClvQpehbjNQDUlYAn6DEpiajoBt18s42PvjfZ9xQmnaZgzrKd18rZPypZP2S-yy7Z20uWt7Y36Wp2qjWDj3YwWTNnF87oFBbsi9XBR-3HeTVUdjc3Px_Scm3nbOv70Znb5-hRp1w0Lw7nCfr54eLH9lO--_rx8_Z8l2sieMpb1nWY14IApo0oNWe45rjVWKnKAO863nIOtGG1aQhfWVoJwRloXmtaNeQEvd_PHaemN602QwrKyTHYXoVZemXlv8lgr-Qv_1tSAgLI4p8e_OBvJhOT7G3Uxjk1GD9FKYgQQEm9gMUeXD8gBtMd38Ag14rkWpHkVFK5VrQIr-9vdsT3nSzxm0O8anfhff30f7nsJueSuU0L-GoPXsfkw5FkjJeU_AWTFq_B</recordid><startdate>19770401</startdate><enddate>19770401</enddate><creator>Politz, Samuel M.</creator><creator>Glitz, Dohn G.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19770401</creationdate><title>Ribosome Structure: Localization of N6,N6-dimethyladenosine by Electron Microscopy of a Ribosome-Antibody Complex</title><author>Politz, Samuel M. ; Glitz, Dohn G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c387t-d6ff17983014b82c761971dc1aa5e07ff7d7704b69eb37ff174588760c79c45b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1977</creationdate><topic>Adenosine - analogs & derivatives</topic><topic>Adenosine - immunology</topic><topic>Albumins</topic><topic>Antibodies</topic><topic>Binding sites</topic><topic>Chemical precipitation</topic><topic>Electron microscopy</topic><topic>Escherichia coli - ultrastructure</topic><topic>Globulins</topic><topic>Haptens</topic><topic>Immunoassay</topic><topic>Kinetics</topic><topic>Microscopy, Electron</topic><topic>Nucleosides</topic><topic>Ribosomes</topic><topic>Ribosomes - immunology</topic><topic>Ribosomes - ultrastructure</topic><topic>RNA</topic><topic>RNA, Ribosomal - analysis</topic><topic>Structure-Activity Relationship</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Politz, Samuel M.</creatorcontrib><creatorcontrib>Glitz, Dohn G.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Politz, Samuel M.</au><au>Glitz, Dohn G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ribosome Structure: Localization of N6,N6-dimethyladenosine by Electron Microscopy of a Ribosome-Antibody Complex</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1977-04-01</date><risdate>1977</risdate><volume>74</volume><issue>4</issue><spage>1468</spage><epage>1472</epage><pages>1468-1472</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Antibodies to the minor nucleoside N6,N6-dimethyladenosine have been used to map a unique location of the nucleoside in the small subunit of the Escherichia coli ribosome. Antibodies were induced in rabbits by a nucleoside-bovine albumin conjugate and shown to be highly specific for the dimethyladenosine hapten. The antibodies were shown to interact with 30S ribosomal subunits from strain PR7, but not with subunits from its mutant strain TPR201, which is resistant to kasugamycin and lacks the two successive residues of dimethyladenosine normally found near the 3′-end of E. coli 16S ribosomal RNA. Electron micrographs of strain PR7 subunits, crosslinked by single IgG molecules, show a single binding site on the surface of the ribosome. This binding site is consistent with observations relating the 3′-end of the ribosomal RNA, binding of initiation factor IF-3 and messenger RNA, and mapping of specific ribosomal proteins.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>323854</pmid><doi>10.1073/pnas.74.4.1468</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Adenosine - analogs & derivatives Adenosine - immunology Albumins Antibodies Binding sites Chemical precipitation Electron microscopy Escherichia coli - ultrastructure Globulins Haptens Immunoassay Kinetics Microscopy, Electron Nucleosides Ribosomes Ribosomes - immunology Ribosomes - ultrastructure RNA RNA, Ribosomal - analysis Structure-Activity Relationship |
title | Ribosome Structure: Localization of N6,N6-dimethyladenosine by Electron Microscopy of a Ribosome-Antibody Complex |
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