Transient Accumulation of Okazaki Fragments as a Result of Uracil Incorporation into Nascent DNA

Strains of Escherichia coli with a mutation in the sof (dnaS) locus show a higher than normal frequency of recombination (are hyper rec) and incorporate label into short (4-5S) DNA fragments following brief [3H]thymidine pulses [Konrad and Lehman, Proc. Natl. Acad. Sci. USA 72, 2150 (1975)]. These m...

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Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 1977-01, Vol.74 (1), p.154-157
Hauptverfasser: Tye, Bik-Kwoon, Nyman, Per-Olof, Lehman, I. R., Hochhauser, Steven, Weiss, Bernard
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container_title Proceedings of the National Academy of Sciences - PNAS
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creator Tye, Bik-Kwoon
Nyman, Per-Olof
Lehman, I. R.
Hochhauser, Steven
Weiss, Bernard
description Strains of Escherichia coli with a mutation in the sof (dnaS) locus show a higher than normal frequency of recombination (are hyper rec) and incorporate label into short (4-5S) DNA fragments following brief [3H]thymidine pulses [Konrad and Lehman, Proc. Natl. Acad. Sci. USA 72, 2150 (1975)]. These mutant strains have now been found to be defective in deoxyuridinetriphosphate diphosphohydrolase (dUTPase; deoxyuridinetriphosphatase, EC 3.6.1.23), the enzyme that catalyzes the hydrolysis of dUTP to dUMP and PPi. Reversion of one sof-mutation to sof+restores dUTPase activity and abolishes the accumulation of labeled 4-5S DNA fragments. Mutants initially isolated as defective in dUTPase (dut-) are also hyper rec and show transient accumulation of short DNA fragments. Both the sof and dut mutations are located at 81 min on the E. coli map, closely linked to the pyrE locus. The sof and dut loci thus appear to be identical. A decrease in dUTPase as a consequence of a sof or dut mutation may result in the increased incorporation of uracil into DNA. Rapid removal of the uracil by an excision-repair process could then lead to the transient accumulation of short DNA fragments. It is possible that at least a portion of the Okazaki fragments seen in wild-type cells may originate in this way.
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subjects Biochemistry
Centrifugation
Chromosome Mapping
Deoxyuridine
Diploidy
DNA
DNA Repair
DNA Replication
DNA, Bacterial - metabolism
Enzymes
Escherichia coli
Genes
Genetic loci
Genetic mutation
Laboratory schools
Molecular Weight
Mutation
Phenotypes
Pyrophosphatases - deficiency
Pyrophosphatases - metabolism
Recombination, Genetic
Uracil Nucleotides - metabolism
title Transient Accumulation of Okazaki Fragments as a Result of Uracil Incorporation into Nascent DNA
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