Antisera against Electrophoretically Purified Tubulin Stimulate Colchicine-Binding Activity
Several rabbit antisera have been prepared against reduced and alkylated, electrophoretically purified tubulin isolated from chick brain. These antisera give a single precipitin line in Ouchterlony double diffusion plates when tested against partially purified tubulin, and label specifically microtu...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1976-04, Vol.73 (4), p.1246-1249 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 73 |
| creator | Aubin, Jane E. Subrahmanyan, Lalitha Kalnins, Vitauts I. Ling, Victor |
| description | Several rabbit antisera have been prepared against reduced and alkylated, electrophoretically purified tubulin isolated from chick brain. These antisera give a single precipitin line in Ouchterlony double diffusion plates when tested against partially purified tubulin, and label specifically microtubule- and tubulin-containing structures, such as mitotic spindles, cilia, and vinblastine-induced crystals, in a variety of cells. The same antisera also display the unique ability to stimulate the colchicine-binding activity of tubulin preparations from chick brain and Chinese hamster ovary tissue culture cells. This specific stimulation of colchicine-binding activity is also obtained with the gamma globulin fractions purified by ammonium sulfate precipitation of these antisera. |
| doi_str_mv | 10.1073/pnas.73.4.1246 |
| format | Article |
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These antisera give a single precipitin line in Ouchterlony double diffusion plates when tested against partially purified tubulin, and label specifically microtubule- and tubulin-containing structures, such as mitotic spindles, cilia, and vinblastine-induced crystals, in a variety of cells. The same antisera also display the unique ability to stimulate the colchicine-binding activity of tubulin preparations from chick brain and Chinese hamster ovary tissue culture cells. This specific stimulation of colchicine-binding activity is also obtained with the gamma globulin fractions purified by ammonium sulfate precipitation of these antisera.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.73.4.1246</identifier><identifier>PMID: 57619</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Animals ; Antibodies ; Antigen-Antibody Reactions ; Antiserum ; Binding Sites ; Chick Embryo ; CHO cells ; Colchicine - metabolism ; Cricetinae ; gamma-Globulins - immunology ; gamma-Globulins - isolation & purification ; Gels ; Globulins ; Glycoproteins - immunology ; HeLa cells ; Immunoglobulins ; Microtubules - immunology ; Mitotic spindle apparatus ; Quaternary ammonium compounds ; Species Specificity ; Sulfates ; Tubulin - immunology</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1976-04, Vol.73 (4), p.1246-1249</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c454t-a8242343907ae23f83ebab967e4645ff3fcc5606efd6047d944436de9fa2572d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/73/4.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/65874$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/65874$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/57619$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Aubin, Jane E.</creatorcontrib><creatorcontrib>Subrahmanyan, Lalitha</creatorcontrib><creatorcontrib>Kalnins, Vitauts I.</creatorcontrib><creatorcontrib>Ling, Victor</creatorcontrib><title>Antisera against Electrophoretically Purified Tubulin Stimulate Colchicine-Binding Activity</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Several rabbit antisera have been prepared against reduced and alkylated, electrophoretically purified tubulin isolated from chick brain. These antisera give a single precipitin line in Ouchterlony double diffusion plates when tested against partially purified tubulin, and label specifically microtubule- and tubulin-containing structures, such as mitotic spindles, cilia, and vinblastine-induced crystals, in a variety of cells. The same antisera also display the unique ability to stimulate the colchicine-binding activity of tubulin preparations from chick brain and Chinese hamster ovary tissue culture cells. This specific stimulation of colchicine-binding activity is also obtained with the gamma globulin fractions purified by ammonium sulfate precipitation of these antisera.</description><subject>Animals</subject><subject>Antibodies</subject><subject>Antigen-Antibody Reactions</subject><subject>Antiserum</subject><subject>Binding Sites</subject><subject>Chick Embryo</subject><subject>CHO cells</subject><subject>Colchicine - metabolism</subject><subject>Cricetinae</subject><subject>gamma-Globulins - immunology</subject><subject>gamma-Globulins - isolation & purification</subject><subject>Gels</subject><subject>Globulins</subject><subject>Glycoproteins - immunology</subject><subject>HeLa cells</subject><subject>Immunoglobulins</subject><subject>Microtubules - immunology</subject><subject>Mitotic spindle apparatus</subject><subject>Quaternary ammonium compounds</subject><subject>Species Specificity</subject><subject>Sulfates</subject><subject>Tubulin - immunology</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1976</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kb9vEzEYhi1EBWlhZQAh3dTtDp_ts88DQ4jKD6kSSLRTB8vxfU5cOXawfRX573shISoL0ze8z-Pvk1-E3rS4abGgH7ZB50bQhjUtYfwZmrVYtjVnEj9HM4yJqHtG2Et0nvM9xlh2PX6BzjrBWzlDd_NQXIakK73SLuRSXXkwJcXtOiYozmjvd9WPMTnrYKhuxuXoXah-FrcZvS5QLaI3a2dcgPqTC4MLq2puintwZfcKnVntM7w-zgt0-_nqZvG1vv7-5dtifl0b1rFS654wQhmVWGgg1PYUlnopuQDGWWcttcZ0HHOwA8dMDJIxRvkA0mrSCTLQC_Tx8O52XG5gMBBK0l5tk9votFNRO_VvEtxareKDYhQTKif_8uin-GuEXNTGZQPe6wBxzKqnVBLRtRPYHECTYs4J7GlHi9W-C7XvQk2TqX0Xk_Du6WUn_M_nT-n7Y7q3_mZP7cv_5cqO3hf4XSbw7QG8zyWmE8m7XjD6CM6aqIY</recordid><startdate>19760401</startdate><enddate>19760401</enddate><creator>Aubin, Jane E.</creator><creator>Subrahmanyan, Lalitha</creator><creator>Kalnins, Vitauts I.</creator><creator>Ling, Victor</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19760401</creationdate><title>Antisera against Electrophoretically Purified Tubulin Stimulate Colchicine-Binding Activity</title><author>Aubin, Jane E. ; Subrahmanyan, Lalitha ; Kalnins, Vitauts I. ; Ling, Victor</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c454t-a8242343907ae23f83ebab967e4645ff3fcc5606efd6047d944436de9fa2572d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1976</creationdate><topic>Animals</topic><topic>Antibodies</topic><topic>Antigen-Antibody Reactions</topic><topic>Antiserum</topic><topic>Binding Sites</topic><topic>Chick Embryo</topic><topic>CHO cells</topic><topic>Colchicine - metabolism</topic><topic>Cricetinae</topic><topic>gamma-Globulins - immunology</topic><topic>gamma-Globulins - isolation & purification</topic><topic>Gels</topic><topic>Globulins</topic><topic>Glycoproteins - immunology</topic><topic>HeLa cells</topic><topic>Immunoglobulins</topic><topic>Microtubules - immunology</topic><topic>Mitotic spindle apparatus</topic><topic>Quaternary ammonium compounds</topic><topic>Species Specificity</topic><topic>Sulfates</topic><topic>Tubulin - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Aubin, Jane E.</creatorcontrib><creatorcontrib>Subrahmanyan, Lalitha</creatorcontrib><creatorcontrib>Kalnins, Vitauts I.</creatorcontrib><creatorcontrib>Ling, Victor</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Aubin, Jane E.</au><au>Subrahmanyan, Lalitha</au><au>Kalnins, Vitauts I.</au><au>Ling, Victor</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antisera against Electrophoretically Purified Tubulin Stimulate Colchicine-Binding Activity</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1976-04-01</date><risdate>1976</risdate><volume>73</volume><issue>4</issue><spage>1246</spage><epage>1249</epage><pages>1246-1249</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Several rabbit antisera have been prepared against reduced and alkylated, electrophoretically purified tubulin isolated from chick brain. These antisera give a single precipitin line in Ouchterlony double diffusion plates when tested against partially purified tubulin, and label specifically microtubule- and tubulin-containing structures, such as mitotic spindles, cilia, and vinblastine-induced crystals, in a variety of cells. The same antisera also display the unique ability to stimulate the colchicine-binding activity of tubulin preparations from chick brain and Chinese hamster ovary tissue culture cells. This specific stimulation of colchicine-binding activity is also obtained with the gamma globulin fractions purified by ammonium sulfate precipitation of these antisera.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>57619</pmid><doi>10.1073/pnas.73.4.1246</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1976-04, Vol.73 (4), p.1246-1249 |
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| language | eng |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Animals Antibodies Antigen-Antibody Reactions Antiserum Binding Sites Chick Embryo CHO cells Colchicine - metabolism Cricetinae gamma-Globulins - immunology gamma-Globulins - isolation & purification Gels Globulins Glycoproteins - immunology HeLa cells Immunoglobulins Microtubules - immunology Mitotic spindle apparatus Quaternary ammonium compounds Species Specificity Sulfates Tubulin - immunology |
| title | Antisera against Electrophoretically Purified Tubulin Stimulate Colchicine-Binding Activity |
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