Biosynthesis of Mouse Interferon by Translation of its Messenger RNA in a Cell-Free System

Biosynthesis of Mouse Interferon by Translation of its Messenger RNA in a Cell-Free System

A fraction of mouse RNA containing messenger RNA coding for mouse interferon was translated with high efficiency in a wheat germ system into a fully active product. This product fulfills the criteria for mouse interferon, namely: (1) it was active against vesicular stomatitis virus and herpes simple...

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Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 1975-10, Vol.72 (10), p.3975-3977
Hauptverfasser: Thang, M. N., Thang, D. C., De Maeyer, E., Montagnier, L.
Format: Artikel
Sprache:eng
Schlagworte:
Antigen-Antibody Reactions
Antivirals
Cell free system
Cell Line
Creatine
Interferons
Interferons - biosynthesis
Interferons - immunology
Interferons - pharmacology
Kinetics
Messenger RNA
Microelectromechanical systems
Plants - metabolism
Protein Biosynthesis
RNA
RNA, Messenger - metabolism
Simplexvirus - drug effects
Species Specificity
Triticum - metabolism
Vero cells
Vesicular stomatitis Indiana virus - drug effects
Viruses
Wheat
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Beschreibung
Zusammenfassung:A fraction of mouse RNA containing messenger RNA coding for mouse interferon was translated with high efficiency in a wheat germ system into a fully active product. This product fulfills the criteria for mouse interferon, namely: (1) it was active against vesicular stomatitis virus and herpes simplex virus in mouse cells; (2) its antiviral activity was species specific; (3) its activity was completely neutralized by mouse anti-interferon serum. The synthesis of interferon in this cell-free system requires the presence of spermine.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.72.10.3975