Induction of Lymphocyte Transformation by Sequential Treatment with Neuraminidase and Galactose Oxidase
Treatment of mouse-spleen cells with galactose oxidase (EC 1.1.3.9) after incubation with neuraminidase (EC 3.2.1.18) induced extensive blastogenesis. Treatment of the cells with galactose oxidase before incubation with neuraminidase had very little stimulatory effect. Either of these enzymes alone...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1973-06, Vol.70 (6), p.1824-1827 |
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| container_end_page | 1827 |
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| container_issue | 6 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 70 |
| creator | Novogrodsky, Abraham Katchalski, Ephraim |
| description | Treatment of mouse-spleen cells with galactose oxidase (EC 1.1.3.9) after incubation with neuraminidase (EC 3.2.1.18) induced extensive blastogenesis. Treatment of the cells with galactose oxidase before incubation with neuraminidase had very little stimulatory effect. Either of these enzymes alone had practically no effect on the cells. The lymphocyte transformation induced by galactose oxidase in neuraminidase-treated cells was decreased upon reacting the cells with borohydride or hydroxylamine, whereas treatment with these compounds did not affect transformation of cells by concanavalin A. It is suggested that galactosyl residues exposed by the action of neuraminidase on the cell membrane are oxidized by galactose oxidase, and the aldehyde moiety thus formed is involved in the induction of blastogenesis. |
| doi_str_mv | 10.1073/pnas.70.6.1824 |
| format | Article |
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Treatment of the cells with galactose oxidase before incubation with neuraminidase had very little stimulatory effect. Either of these enzymes alone had practically no effect on the cells. The lymphocyte transformation induced by galactose oxidase in neuraminidase-treated cells was decreased upon reacting the cells with borohydride or hydroxylamine, whereas treatment with these compounds did not affect transformation of cells by concanavalin A. 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Treatment of the cells with galactose oxidase before incubation with neuraminidase had very little stimulatory effect. Either of these enzymes alone had practically no effect on the cells. The lymphocyte transformation induced by galactose oxidase in neuraminidase-treated cells was decreased upon reacting the cells with borohydride or hydroxylamine, whereas treatment with these compounds did not affect transformation of cells by concanavalin A. It is suggested that galactosyl residues exposed by the action of neuraminidase on the cell membrane are oxidized by galactose oxidase, and the aldehyde moiety thus formed is involved in the induction of blastogenesis.</description><subject>Alcohol Oxidoreductases - antagonists & inhibitors</subject><subject>Alcohol Oxidoreductases - pharmacology</subject><subject>Aldehydes</subject><subject>Animals</subject><subject>Biological Sciences: Biochemistry</subject><subject>Borohydrides</subject><subject>Borohydrides - pharmacology</subject><subject>Carbamates - pharmacology</subject><subject>Cell membranes</subject><subject>Concanavalin A - pharmacology</subject><subject>Cultured cells</subject><subject>Drug Synergism</subject><subject>Enzymes</subject><subject>Galactose - pharmacology</subject><subject>Hydroxylamines - pharmacology</subject><subject>Lymphocyte activation</subject><subject>Lymphocyte Activation - drug effects</subject><subject>Lymphocytes</subject><subject>Lymphocytes - metabolism</subject><subject>Mice</subject><subject>Neuraminidase - antagonists & inhibitors</subject><subject>Neuraminidase - pharmacology</subject><subject>Neurons</subject><subject>Oxidases</subject><subject>Periodic Acid - pharmacology</subject><subject>Spleen - cytology</subject><subject>Spleen - immunology</subject><subject>Spleen cells</subject><subject>Thymidine - metabolism</subject><subject>Tritium</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1973</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkE1P3DAURS3Uig60WxaVKmXVXdLnjzjJootqVAakESyAtfXiOExQEk9th07-fRNmGFGJlfV87nm2LiEXFBIKGf-x7dEnGSQyoTkTJ2RBoaCxFAV8IAsAlsW5YOITOfP-CQCKNIdTcip4ymQqF-Txuq8GHRrbR7aO1mO33Vg9BhPdO-x9bV2HL7AcozvzZzB9aLCdoMHQTUP0twmb6MYMDrumbyr0JsK-ilbYog52mm53L7efyccaW2--HM5z8nD5-355Fa9vV9fLX-tYi1SGmFYcSoGSg8HS1EChLIyoBc3qPEeqK1lJYAbEHGJFqTXwjGW6oKwQrND8nPzc790OZWcqPf3RYau2runQjcpio_4nfbNRj_ZZCc4lpJOf7H3trPfO1EeVgpoLV3PhKgMl1Vz4JHx7--Axfmh44t8PfPZe6dFX9dC2wezCm0XvBif-dc-ffLDuGJCMsZz_A_0DoFE</recordid><startdate>19730601</startdate><enddate>19730601</enddate><creator>Novogrodsky, Abraham</creator><creator>Katchalski, Ephraim</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>19730601</creationdate><title>Induction of Lymphocyte Transformation by Sequential Treatment with Neuraminidase and Galactose Oxidase</title><author>Novogrodsky, Abraham ; Katchalski, Ephraim</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c456t-1d30b4a630eabef010b9e4f417f88a1cd6d602e04b4a629bcc03727c9129429c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1973</creationdate><topic>Alcohol Oxidoreductases - antagonists & inhibitors</topic><topic>Alcohol Oxidoreductases - pharmacology</topic><topic>Aldehydes</topic><topic>Animals</topic><topic>Biological Sciences: Biochemistry</topic><topic>Borohydrides</topic><topic>Borohydrides - pharmacology</topic><topic>Carbamates - pharmacology</topic><topic>Cell membranes</topic><topic>Concanavalin A - pharmacology</topic><topic>Cultured cells</topic><topic>Drug Synergism</topic><topic>Enzymes</topic><topic>Galactose - pharmacology</topic><topic>Hydroxylamines - pharmacology</topic><topic>Lymphocyte activation</topic><topic>Lymphocyte Activation - drug effects</topic><topic>Lymphocytes</topic><topic>Lymphocytes - metabolism</topic><topic>Mice</topic><topic>Neuraminidase - antagonists & inhibitors</topic><topic>Neuraminidase - pharmacology</topic><topic>Neurons</topic><topic>Oxidases</topic><topic>Periodic Acid - pharmacology</topic><topic>Spleen - cytology</topic><topic>Spleen - immunology</topic><topic>Spleen cells</topic><topic>Thymidine - metabolism</topic><topic>Tritium</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Novogrodsky, Abraham</creatorcontrib><creatorcontrib>Katchalski, Ephraim</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Novogrodsky, Abraham</au><au>Katchalski, Ephraim</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Induction of Lymphocyte Transformation by Sequential Treatment with Neuraminidase and Galactose Oxidase</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1973-06-01</date><risdate>1973</risdate><volume>70</volume><issue>6</issue><spage>1824</spage><epage>1827</epage><pages>1824-1827</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Treatment of mouse-spleen cells with galactose oxidase (EC 1.1.3.9) after incubation with neuraminidase (EC 3.2.1.18) induced extensive blastogenesis. Treatment of the cells with galactose oxidase before incubation with neuraminidase had very little stimulatory effect. Either of these enzymes alone had practically no effect on the cells. The lymphocyte transformation induced by galactose oxidase in neuraminidase-treated cells was decreased upon reacting the cells with borohydride or hydroxylamine, whereas treatment with these compounds did not affect transformation of cells by concanavalin A. It is suggested that galactosyl residues exposed by the action of neuraminidase on the cell membrane are oxidized by galactose oxidase, and the aldehyde moiety thus formed is involved in the induction of blastogenesis.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>4352656</pmid><doi>10.1073/pnas.70.6.1824</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1973-06, Vol.70 (6), p.1824-1827 |
| issn | 0027-8424 1091-6490 |
| language | eng |
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| source | MEDLINE; Jstor Complete Legacy; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Alcohol Oxidoreductases - antagonists & inhibitors Alcohol Oxidoreductases - pharmacology Aldehydes Animals Biological Sciences: Biochemistry Borohydrides Borohydrides - pharmacology Carbamates - pharmacology Cell membranes Concanavalin A - pharmacology Cultured cells Drug Synergism Enzymes Galactose - pharmacology Hydroxylamines - pharmacology Lymphocyte activation Lymphocyte Activation - drug effects Lymphocytes Lymphocytes - metabolism Mice Neuraminidase - antagonists & inhibitors Neuraminidase - pharmacology Neurons Oxidases Periodic Acid - pharmacology Spleen - cytology Spleen - immunology Spleen cells Thymidine - metabolism Tritium |
| title | Induction of Lymphocyte Transformation by Sequential Treatment with Neuraminidase and Galactose Oxidase |
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