Demonstration of Globin Messenger Sequences in Giant Nuclear Precursors of Messenger RNA of Avian Erythroblasts
Highly purified globin mRNA from ducks was copied with RNA-directed DNA polymerase from avian myeloblastosis virus into anti-messenger DNA. With excess RNA, more than 90% of this DNA annealed back to its template with a Cot/2 value of 7.5 × 10-4mol· sec· liter-1; the melting temperature of the hybri...
Gespeichert in:
| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1973-04, Vol.70 (4), p.1122-1126 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1126 |
|---|---|
| container_issue | 4 |
| container_start_page | 1122 |
| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 70 |
| creator | Imaizumi, Tereza Diggelmann, Heidi Scherrer, Klaus |
| description | Highly purified globin mRNA from ducks was copied with RNA-directed DNA polymerase from avian myeloblastosis virus into anti-messenger DNA. With excess RNA, more than 90% of this DNA annealed back to its template with a Cot/2 value of 7.5 × 10-4mol· sec· liter-1; the melting temperature of the hybrid was 86 degrees. Giant nuclear RNA fractions with sedimentation coefficients of more than 50 S formed hybrids of almost equal stability at Cot/2 values of 0.05-0.42 mol· sec· liter-1, indicating amRNA content of 0.3-1.5%. 12S RNA from the same polyribosomes and nuclear giant RNA from HeLa cells did not cross-hybridize. Although a large part of the giant RNA broke down in 99% dimethylsulfoxide gradients, RNA fractions sedimenting faster than 28S rRNA still were found to consist of up to 0.03% globin mRNA sequences. Thus, the mRNA sequences are contained in the covalent structure of giant nuclear precursors, which are termed precursor-mRNA. |
| doi_str_mv | 10.1073/pnas.70.4.1122 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_pnas_</sourceid><recordid>TN_cdi_pnas_primary_70_4_1122</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>62437</jstor_id><sourcerecordid>62437</sourcerecordid><originalsourceid>FETCH-LOGICAL-c456t-adf54848224342da619cb0212a88757e70515e24b97969977b7942ef68af98eb3</originalsourceid><addsrcrecordid>eNptkc1v1DAQxS0EKsvClQMSUk7cEvyV2D5wWJWyIJWC-DhbTnbSpsraW49T0f8eR7ssi8TJ0rz3m_HMI-QloxWjSrzdeYeVopWsGOP8EVkwaljZSEMfkwWlXJVacvmUPEO8pZSaWtMzciYZF0bQBQnvYRs8pujSEHwR-mI9hnbwxWdABH8NsfgOdxP4DrDI5fXgfCqupm4EF4uvEbopYog4k3-Rb1erubC6z-7iIj6kmxja0WHC5-RJ70aEF4d3SX5-uPhx_rG8_LL-dL66LDtZN6l0m76WWmrOpZB84xpmupZyxp3WqlagaM1q4LI1yjTGKNUqIzn0jXa90dCKJXm377ub2i1sOvB5xdHu4rB18cEGN9h_FT_c2Otwb6UQMp9mSd4c-Bjy-pjsdsAOxtF5CBNazUyjaSOysdobuxgQI_THGYzaOSE7J2QVtdLOCWXg9enPjvZDJCeTZ-6PeuRtP41jgl_ppNF_jVl_tddvMYV4NDT5pEr8Bkd7ro0</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>81968063</pqid></control><display><type>article</type><title>Demonstration of Globin Messenger Sequences in Giant Nuclear Precursors of Messenger RNA of Avian Erythroblasts</title><source>MEDLINE</source><source>JSTOR Archive Collection A-Z Listing</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>Imaizumi, Tereza ; Diggelmann, Heidi ; Scherrer, Klaus</creator><creatorcontrib>Imaizumi, Tereza ; Diggelmann, Heidi ; Scherrer, Klaus</creatorcontrib><description>Highly purified globin mRNA from ducks was copied with RNA-directed DNA polymerase from avian myeloblastosis virus into anti-messenger DNA. With excess RNA, more than 90% of this DNA annealed back to its template with a Cot/2 value of 7.5 × 10-4mol· sec· liter-1; the melting temperature of the hybrid was 86 degrees. Giant nuclear RNA fractions with sedimentation coefficients of more than 50 S formed hybrids of almost equal stability at Cot/2 values of 0.05-0.42 mol· sec· liter-1, indicating amRNA content of 0.3-1.5%. 12S RNA from the same polyribosomes and nuclear giant RNA from HeLa cells did not cross-hybridize. Although a large part of the giant RNA broke down in 99% dimethylsulfoxide gradients, RNA fractions sedimenting faster than 28S rRNA still were found to consist of up to 0.03% globin mRNA sequences. Thus, the mRNA sequences are contained in the covalent structure of giant nuclear precursors, which are termed precursor-mRNA.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.70.4.1122</identifier><identifier>PMID: 4123930</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Animals ; Avian Leukosis Virus - enzymology ; Avian Leukosis Virus - metabolism ; Base Sequence ; Biological Sciences: Biochemistry ; Centrifugation, Density Gradient ; DNA ; DNA, Viral - biosynthesis ; DNA, Viral - metabolism ; Ducks ; Erythroblasts ; Erythrocytes - metabolism ; Globins - biosynthesis ; Hybridity ; Kinetics ; Messenger RNA ; Molecular Weight ; Molecules ; Nucleic Acid Hybridization ; Nucleotide sequences ; Nucleotides - analysis ; RNA ; RNA precursors ; RNA, Messenger - analysis ; RNA, Messenger - isolation & purification ; RNA, Messenger - metabolism ; RNA-Directed DNA Polymerase - metabolism ; Templates, Genetic</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1973-04, Vol.70 (4), p.1122-1126</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c456t-adf54848224342da619cb0212a88757e70515e24b97969977b7942ef68af98eb3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/70/4.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/62437$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/62437$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4123930$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Imaizumi, Tereza</creatorcontrib><creatorcontrib>Diggelmann, Heidi</creatorcontrib><creatorcontrib>Scherrer, Klaus</creatorcontrib><title>Demonstration of Globin Messenger Sequences in Giant Nuclear Precursors of Messenger RNA of Avian Erythroblasts</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Highly purified globin mRNA from ducks was copied with RNA-directed DNA polymerase from avian myeloblastosis virus into anti-messenger DNA. With excess RNA, more than 90% of this DNA annealed back to its template with a Cot/2 value of 7.5 × 10-4mol· sec· liter-1; the melting temperature of the hybrid was 86 degrees. Giant nuclear RNA fractions with sedimentation coefficients of more than 50 S formed hybrids of almost equal stability at Cot/2 values of 0.05-0.42 mol· sec· liter-1, indicating amRNA content of 0.3-1.5%. 12S RNA from the same polyribosomes and nuclear giant RNA from HeLa cells did not cross-hybridize. Although a large part of the giant RNA broke down in 99% dimethylsulfoxide gradients, RNA fractions sedimenting faster than 28S rRNA still were found to consist of up to 0.03% globin mRNA sequences. Thus, the mRNA sequences are contained in the covalent structure of giant nuclear precursors, which are termed precursor-mRNA.</description><subject>Animals</subject><subject>Avian Leukosis Virus - enzymology</subject><subject>Avian Leukosis Virus - metabolism</subject><subject>Base Sequence</subject><subject>Biological Sciences: Biochemistry</subject><subject>Centrifugation, Density Gradient</subject><subject>DNA</subject><subject>DNA, Viral - biosynthesis</subject><subject>DNA, Viral - metabolism</subject><subject>Ducks</subject><subject>Erythroblasts</subject><subject>Erythrocytes - metabolism</subject><subject>Globins - biosynthesis</subject><subject>Hybridity</subject><subject>Kinetics</subject><subject>Messenger RNA</subject><subject>Molecular Weight</subject><subject>Molecules</subject><subject>Nucleic Acid Hybridization</subject><subject>Nucleotide sequences</subject><subject>Nucleotides - analysis</subject><subject>RNA</subject><subject>RNA precursors</subject><subject>RNA, Messenger - analysis</subject><subject>RNA, Messenger - isolation & purification</subject><subject>RNA, Messenger - metabolism</subject><subject>RNA-Directed DNA Polymerase - metabolism</subject><subject>Templates, Genetic</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1973</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkc1v1DAQxS0EKsvClQMSUk7cEvyV2D5wWJWyIJWC-DhbTnbSpsraW49T0f8eR7ssi8TJ0rz3m_HMI-QloxWjSrzdeYeVopWsGOP8EVkwaljZSEMfkwWlXJVacvmUPEO8pZSaWtMzciYZF0bQBQnvYRs8pujSEHwR-mI9hnbwxWdABH8NsfgOdxP4DrDI5fXgfCqupm4EF4uvEbopYog4k3-Rb1erubC6z-7iIj6kmxja0WHC5-RJ70aEF4d3SX5-uPhx_rG8_LL-dL66LDtZN6l0m76WWmrOpZB84xpmupZyxp3WqlagaM1q4LI1yjTGKNUqIzn0jXa90dCKJXm377ub2i1sOvB5xdHu4rB18cEGN9h_FT_c2Otwb6UQMp9mSd4c-Bjy-pjsdsAOxtF5CBNazUyjaSOysdobuxgQI_THGYzaOSE7J2QVtdLOCWXg9enPjvZDJCeTZ-6PeuRtP41jgl_ppNF_jVl_tddvMYV4NDT5pEr8Bkd7ro0</recordid><startdate>19730401</startdate><enddate>19730401</enddate><creator>Imaizumi, Tereza</creator><creator>Diggelmann, Heidi</creator><creator>Scherrer, Klaus</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19730401</creationdate><title>Demonstration of Globin Messenger Sequences in Giant Nuclear Precursors of Messenger RNA of Avian Erythroblasts</title><author>Imaizumi, Tereza ; Diggelmann, Heidi ; Scherrer, Klaus</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c456t-adf54848224342da619cb0212a88757e70515e24b97969977b7942ef68af98eb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1973</creationdate><topic>Animals</topic><topic>Avian Leukosis Virus - enzymology</topic><topic>Avian Leukosis Virus - metabolism</topic><topic>Base Sequence</topic><topic>Biological Sciences: Biochemistry</topic><topic>Centrifugation, Density Gradient</topic><topic>DNA</topic><topic>DNA, Viral - biosynthesis</topic><topic>DNA, Viral - metabolism</topic><topic>Ducks</topic><topic>Erythroblasts</topic><topic>Erythrocytes - metabolism</topic><topic>Globins - biosynthesis</topic><topic>Hybridity</topic><topic>Kinetics</topic><topic>Messenger RNA</topic><topic>Molecular Weight</topic><topic>Molecules</topic><topic>Nucleic Acid Hybridization</topic><topic>Nucleotide sequences</topic><topic>Nucleotides - analysis</topic><topic>RNA</topic><topic>RNA precursors</topic><topic>RNA, Messenger - analysis</topic><topic>RNA, Messenger - isolation & purification</topic><topic>RNA, Messenger - metabolism</topic><topic>RNA-Directed DNA Polymerase - metabolism</topic><topic>Templates, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Imaizumi, Tereza</creatorcontrib><creatorcontrib>Diggelmann, Heidi</creatorcontrib><creatorcontrib>Scherrer, Klaus</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Imaizumi, Tereza</au><au>Diggelmann, Heidi</au><au>Scherrer, Klaus</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Demonstration of Globin Messenger Sequences in Giant Nuclear Precursors of Messenger RNA of Avian Erythroblasts</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1973-04-01</date><risdate>1973</risdate><volume>70</volume><issue>4</issue><spage>1122</spage><epage>1126</epage><pages>1122-1126</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Highly purified globin mRNA from ducks was copied with RNA-directed DNA polymerase from avian myeloblastosis virus into anti-messenger DNA. With excess RNA, more than 90% of this DNA annealed back to its template with a Cot/2 value of 7.5 × 10-4mol· sec· liter-1; the melting temperature of the hybrid was 86 degrees. Giant nuclear RNA fractions with sedimentation coefficients of more than 50 S formed hybrids of almost equal stability at Cot/2 values of 0.05-0.42 mol· sec· liter-1, indicating amRNA content of 0.3-1.5%. 12S RNA from the same polyribosomes and nuclear giant RNA from HeLa cells did not cross-hybridize. Although a large part of the giant RNA broke down in 99% dimethylsulfoxide gradients, RNA fractions sedimenting faster than 28S rRNA still were found to consist of up to 0.03% globin mRNA sequences. Thus, the mRNA sequences are contained in the covalent structure of giant nuclear precursors, which are termed precursor-mRNA.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>4123930</pmid><doi>10.1073/pnas.70.4.1122</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1973-04, Vol.70 (4), p.1122-1126 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_pnas_primary_70_4_1122 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Animals Avian Leukosis Virus - enzymology Avian Leukosis Virus - metabolism Base Sequence Biological Sciences: Biochemistry Centrifugation, Density Gradient DNA DNA, Viral - biosynthesis DNA, Viral - metabolism Ducks Erythroblasts Erythrocytes - metabolism Globins - biosynthesis Hybridity Kinetics Messenger RNA Molecular Weight Molecules Nucleic Acid Hybridization Nucleotide sequences Nucleotides - analysis RNA RNA precursors RNA, Messenger - analysis RNA, Messenger - isolation & purification RNA, Messenger - metabolism RNA-Directed DNA Polymerase - metabolism Templates, Genetic |
| title | Demonstration of Globin Messenger Sequences in Giant Nuclear Precursors of Messenger RNA of Avian Erythroblasts |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T09%3A03%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_pnas_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Demonstration%20of%20Globin%20Messenger%20Sequences%20in%20Giant%20Nuclear%20Precursors%20of%20Messenger%20RNA%20of%20Avian%20Erythroblasts&rft.jtitle=Proceedings%20of%20the%20National%20Academy%20of%20Sciences%20-%20PNAS&rft.au=Imaizumi,%20Tereza&rft.date=1973-04-01&rft.volume=70&rft.issue=4&rft.spage=1122&rft.epage=1126&rft.pages=1122-1126&rft.issn=0027-8424&rft.eissn=1091-6490&rft_id=info:doi/10.1073/pnas.70.4.1122&rft_dat=%3Cjstor_pnas_%3E62437%3C/jstor_pnas_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=81968063&rft_id=info:pmid/4123930&rft_jstor_id=62437&rfr_iscdi=true |