Three-dimensional bioprinting of thick vascularized tissues
The advancement of tissue and, ultimately, organ engineering requires the ability to pattern human tissues composed of cells, extracellular matrix, and vasculature with controlled microenvironments that can be sustained over prolonged time periods. To date, bioprinting methods have yielded thin tiss...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2016-03, Vol.113 (12), p.3179-3184 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Kolesky, David B. Homan, Kimberly A. Skylar-Scott, Mark A. Lewis, Jennifer A. |
| description | The advancement of tissue and, ultimately, organ engineering requires the ability to pattern human tissues composed of cells, extracellular matrix, and vasculature with controlled microenvironments that can be sustained over prolonged time periods. To date, bioprinting methods have yielded thin tissues that only survive for short durations. To improve their physiological relevance, we report a method for bioprinting 3D cell-laden, vascularized tissues that exceed 1 cm in thickness and can be perfused on chip for long time periods (>6 wk). Specifically, we integrate parenchyma, stroma, and endothelium into a single thick tissue by coprinting multiple inks composed of human mesenchymal stem cells (hMSCs) and human neonatal dermal fibroblasts (hNDFs) within a customized extracellular matrix alongside embedded vasculature, which is subsequently lined with human umbilical vein endothelial cells (HUVECs). These thick vascularized tissues are actively perfused with growth factors to differentiate hMSCs toward an osteogenic lineage in situ. This longitudinal study of emergent biological phenomena in complex microenvironments represents a foundational step in human tissue generation. |
| doi_str_mv | 10.1073/pnas.1521342113 |
| format | Article |
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| language | eng |
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| source | Jstor Complete Legacy; MEDLINE; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Blood Vessels Human Umbilical Vein Endothelial Cells Humans Physical Sciences Printing, Three-Dimensional Stem cells Tissues Veins & arteries |
| title | Three-dimensional bioprinting of thick vascularized tissues |
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