KH domain protein RCF3 is a tissue-biased regulator of the plant miRNA biogenesis cofactor HYL1
The biogenesis of microRNAs (miRNAs), which regulate mRNA abundance through posttranscriptional silencing, comprises multiple well-orchestrated processing steps. We have identified theArabidopsis thalianaK homology (KH) domain protein REGULATOR OF CBF GENE EXPRESSION 3 (RCF3) as a cofactor affecting...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2015-11, Vol.112 (45), p.14096-14101 |
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| container_issue | 45 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Karlsson, Patricia Christie, Michael Danger Seymour, Danelle K. Wang, Huan Wang, Xi Hagmann, Jörg Kulcheski, Franceli Manavella, Pablo Andrés |
| description | The biogenesis of microRNAs (miRNAs), which regulate mRNA abundance through posttranscriptional silencing, comprises multiple well-orchestrated processing steps. We have identified theArabidopsis thalianaK homology (KH) domain protein REGULATOR OF CBF GENE EXPRESSION 3 (RCF3) as a cofactor affecting miRNA biogenesis in specific plant tissues. MiRNA and miRNA-target levels were reduced in apex-enriched samples ofrcf3mutants, but not in other tissues. Mechanistically, RCF3 affects miRNA biogenesis through nuclear interactions with the phosphatases C-TERMINAL DOMAIN PHOSPHATASE-LIKE1 and 2 (CPL1 and CPL2). These interactions are essential to regulate the phosphorylation status, and thus the activity, of the double-stranded RNA binding protein and DICER-LIKE1 (DCL1) cofactor HYPONASTIC LEAVES1 (HYL1). |
| doi_str_mv | 10.1073/pnas.1512865112 |
| format | Article |
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We have identified theArabidopsis thalianaK homology (KH) domain protein REGULATOR OF CBF GENE EXPRESSION 3 (RCF3) as a cofactor affecting miRNA biogenesis in specific plant tissues. MiRNA and miRNA-target levels were reduced in apex-enriched samples ofrcf3mutants, but not in other tissues. Mechanistically, RCF3 affects miRNA biogenesis through nuclear interactions with the phosphatases C-TERMINAL DOMAIN PHOSPHATASE-LIKE1 and 2 (CPL1 and CPL2). 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We have identified theArabidopsis thalianaK homology (KH) domain protein REGULATOR OF CBF GENE EXPRESSION 3 (RCF3) as a cofactor affecting miRNA biogenesis in specific plant tissues. MiRNA and miRNA-target levels were reduced in apex-enriched samples ofrcf3mutants, but not in other tissues. Mechanistically, RCF3 affects miRNA biogenesis through nuclear interactions with the phosphatases C-TERMINAL DOMAIN PHOSPHATASE-LIKE1 and 2 (CPL1 and CPL2). 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| subjects | Arabidopsis - metabolism Arabidopsis Proteins - metabolism Arabidopsis thaliana Base Sequence Biological Sciences Biosynthesis DNA Primers - genetics Flowers & plants Luciferases MicroRNAs - biosynthesis Molecular Sequence Data Mutation Proteins Reverse Transcriptase Polymerase Chain Reaction Ribonucleic acid RNA RNA-Binding Proteins - metabolism Sequence Analysis, DNA Tissues Transgenes - genetics |
| title | KH domain protein RCF3 is a tissue-biased regulator of the plant miRNA biogenesis cofactor HYL1 |
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