Enhancement of protein expression by alphavirus replicons by designing self-replicating subgenomic RNAs

Since the development of infectious cDNA clones of viral RNA genomes and the means of delivery of the in vitro-synthesized RNA into cells, alphaviruses have become an attractive system for expression of heterologous genetic information. Alphaviruses replicate exclusively in the cytoplasm, and their...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 2014-07, Vol.111 (29), p.10708-10713
Hauptverfasser:	Kim, Dal Young, Atasheva, Svetlana, McAuley, Alexander J., Plante, Jessica A., Frolova, Elena I., Beasley, David W. C., Frolov, Ilya
Format:	Artikel
Sprache:	eng
Schlagworte:	Alphavirus Alphavirus - drug effects Alphavirus - genetics Alphaviruses Animals Antibodies, Neutralizing - pharmacology Biological Sciences Capsid proteins Cells clones complementary DNA Cytoplasm Encephalitis Virus, Venezuelan Equine - drug effects Encephalitis Virus, Venezuelan Equine - physiology Enzymes Gene Expression Genetic Vectors Genome, Viral - genetics Genomes Genomics Green Fluorescent Proteins - metabolism heterologous gene expression immunization Interferon-beta - pharmacology Intracellular Space - metabolism Mice neutralizing antibodies Protein Biosynthesis - drug effects Protein expression Protein synthesis proteins Replicon Replicon - genetics Ribonucleic acid RNA RNA Interference - drug effects RNA replication RNA, Viral - genetics RNA, Viral - metabolism structural genes Vaccination Venezuelan equine encephalitis virus Venezuelan equine encephalomyelitis Viral Proteins - metabolism Viral Proteins - ultrastructure Virus Replication - drug effects Virus Replication - genetics Viruses West Nile virus West Nile virus - drug effects West Nile virus - physiology
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