Ectopic assembly of heterochromatin in Drosophila melanogaster triggered by transposable elements
A persistent question in biology is how cis -acting sequence elements influence trans -acting factors and the local chromatin environment to modulate gene expression. We reported previously that the DNA transposon 1360 can enhance silencing of a reporter in a heterochromatic domain of Drosophila mel...
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Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2012-08, Vol.109 (35), p.14104-14109 |
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description | A persistent question in biology is how cis -acting sequence elements influence trans -acting factors and the local chromatin environment to modulate gene expression. We reported previously that the DNA transposon 1360 can enhance silencing of a reporter in a heterochromatic domain of Drosophila melanogaster . We have now generated a collection of variegating phiC31 landing-pad insertion lines containing 1360 and a heat-shock protein 70 (hsp70)-driven white reporter to explore the mechanism of 1360 -sensitive silencing. Many 1360 -sensitive sites were identified, some in apparently euchromatic domains, although all are close to heterochromatic masses. One such site (line 1198; insertion near the base of chromosome arm 2L) has been investigated in detail. ChIP analysis shows 1360 -dependent Heterochromatin Protein 1a (HP1a) accumulation at this otherwise euchromatic site. The phiC31 landing pad system allows different 1360 constructs to be swapped with the full-length element at the same genomic site to identify the sequences that mediate 1360 -sensitive silencing. Short deletions over sites with homology to PIWI-interacting RNAs (piRNAs) are sufficient to compromise 1360 -sensitive silencing. Similar results were obtained on replacing 1360 with Invader4 (a retrotransposon), suggesting that this phenomenon likely applies to a broader set of transposable elements. Our results suggest a model in which piRNA sequence elements behave as cis -acting targets for heterochromatin assembly, likely in the early embryo, where piRNA pathway components are abundant, with the heterochromatic state subsequently propagated by chromatin modifiers present in somatic tissue. |
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The phiC31 landing pad system allows different 1360 constructs to be swapped with the full-length element at the same genomic site to identify the sequences that mediate 1360 -sensitive silencing. Short deletions over sites with homology to PIWI-interacting RNAs (piRNAs) are sufficient to compromise 1360 -sensitive silencing. Similar results were obtained on replacing 1360 with Invader4 (a retrotransposon), suggesting that this phenomenon likely applies to a broader set of transposable elements. 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R</creatorcontrib><title>Ectopic assembly of heterochromatin in Drosophila melanogaster triggered by transposable elements</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>A persistent question in biology is how cis -acting sequence elements influence trans -acting factors and the local chromatin environment to modulate gene expression. We reported previously that the DNA transposon 1360 can enhance silencing of a reporter in a heterochromatic domain of Drosophila melanogaster . We have now generated a collection of variegating phiC31 landing-pad insertion lines containing 1360 and a heat-shock protein 70 (hsp70)-driven white reporter to explore the mechanism of 1360 -sensitive silencing. Many 1360 -sensitive sites were identified, some in apparently euchromatic domains, although all are close to heterochromatic masses. One such site (line 1198; insertion near the base of chromosome arm 2L) has been investigated in detail. ChIP analysis shows 1360 -dependent Heterochromatin Protein 1a (HP1a) accumulation at this otherwise euchromatic site. The phiC31 landing pad system allows different 1360 constructs to be swapped with the full-length element at the same genomic site to identify the sequences that mediate 1360 -sensitive silencing. Short deletions over sites with homology to PIWI-interacting RNAs (piRNAs) are sufficient to compromise 1360 -sensitive silencing. Similar results were obtained on replacing 1360 with Invader4 (a retrotransposon), suggesting that this phenomenon likely applies to a broader set of transposable elements. 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Similar results were obtained on replacing 1360 with Invader4 (a retrotransposon), suggesting that this phenomenon likely applies to a broader set of transposable elements. Our results suggest a model in which piRNA sequence elements behave as cis -acting targets for heterochromatin assembly, likely in the early embryo, where piRNA pathway components are abundant, with the heterochromatic state subsequently propagated by chromatin modifiers present in somatic tissue.</abstract><cop>United States</cop><pub>National Academy of Sciences</pub><pmid>22891327</pmid><doi>10.1073/pnas.1207036109</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Animals Biological Sciences Chromatin Chromosomes Deoxyribonucleic acid DNA DNA Transposable Elements - genetics Drosophila Drosophila melanogaster Drosophila melanogaster - genetics Embryos Epigenesis, Genetic - genetics Female Gene expression Gene Silencing Genes, Insect - genetics Genes, Reporter Genetic Complementation Test Genomes Germ cells Heat shock proteins heat-shock protein 70 Heterochromatin Heterochromatin - genetics Insects Journalism Male Oligonucleotide Array Sequence Analysis retrotransposons Ribonucleic acid RNA RNA, Antisense - genetics RNA, Small Interfering - genetics somatic cells Stem cells Transcription Initiation Site Transposons |
title | Ectopic assembly of heterochromatin in Drosophila melanogaster triggered by transposable elements |
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