Functional genome analysis of Bifidobacterium breve UCC2003 reveals type IVb tight adherence (Tad) pili as an essential and conserved host-colonization factor
Development of the human gut microbiota commences at birth, with bifidobacteria being among the first colonizers of the sterile newborn gastrointestinal tract. To date, the genetic basis of Bifidobacterium colonization and persistence remains poorly understood. Transcriptome analysis of the Bifidoba...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2011-07, Vol.108 (27), p.11217-11222 |
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| creator | O'Connell Motherway, Mary Zomer, Aldert Leahy, Sinead C Reunanen, Justus Bottacini, Francesca Claesson, Marcus J O'Brien, Frances Flynn, Kiera Casey, Patrick G Moreno Munoz, Jose Antonio Kearney, Breda Houston, Aileen M O'Mahony, Caitlin Higgins, Des G Shanahan, Fergus Palva, Airi de Vos, Willem M Fitzgerald, Gerald F Ventura, Marco O'Toole, Paul W van Sinderen, Douwe |
| description | Development of the human gut microbiota commences at birth, with bifidobacteria being among the first colonizers of the sterile newborn gastrointestinal tract. To date, the genetic basis of Bifidobacterium colonization and persistence remains poorly understood. Transcriptome analysis of the Bifidobacterium breve UCC2003 2.42-Mb genome in a murine colonization model revealed differential expression of a type IVb tight adherence (Tad) pilus-encoding gene cluster designated "tadââââ." Mutational analysis demonstrated that the tadââââ gene cluster is essential for efficient in vivo murine gut colonization, and immunogold transmission electron microscopy confirmed the presence of Tad pili at the poles of B. breve UCC2003 cells. Conservation of the Tad pilus-encoding locus among other B. breve strains and among sequenced Bifidobacterium genomes supports the notion of a ubiquitous pili-mediated host colonization and persistence mechanism for bifidobacteria. |
| doi_str_mv | 10.1073/pnas.1105380108 |
| format | Article |
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To date, the genetic basis of Bifidobacterium colonization and persistence remains poorly understood. Transcriptome analysis of the Bifidobacterium breve UCC2003 2.42-Mb genome in a murine colonization model revealed differential expression of a type IVb tight adherence (Tad) pilus-encoding gene cluster designated "tadââââ." Mutational analysis demonstrated that the tadââââ gene cluster is essential for efficient in vivo murine gut colonization, and immunogold transmission electron microscopy confirmed the presence of Tad pili at the poles of B. breve UCC2003 cells. Conservation of the Tad pilus-encoding locus among other B. breve strains and among sequenced Bifidobacterium genomes supports the notion of a ubiquitous pili-mediated host colonization and persistence mechanism for bifidobacteria.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.1105380108</identifier><identifier>PMID: 21690406</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>actinobacillus-actinomycetemcomitans ; Amino Acid Sequence ; Animals ; Bacterial Proteins - genetics ; Bacterial Proteins - physiology ; Base Sequence ; Bifidobacterium ; Bifidobacterium - genetics ; Bifidobacterium - growth & development ; Bifidobacterium - physiology ; Bifidobacterium - ultrastructure ; Bifidobacterium breve ; Biological Sciences ; Birth ; Colonization ; Comparative Genomic Hybridization ; Conservation ; Conserved sequence ; Digestive tract ; DNA, Bacterial - genetics ; Female ; fimbriae ; Fimbriae, Bacterial - genetics ; Fimbriae, Bacterial - physiology ; Fimbriae, Bacterial - ultrastructure ; formula-fed infants ; gastrointestinal system ; Gastrointestinal tract ; Gastrointestinal Tract - microbiology ; Gene clusters ; Gene expression ; gene expression regulation ; Gene Expression Regulation, Bacterial ; Genes ; Genetic loci ; Genome, Bacterial ; Genomes ; Genomics ; Germ-Free Life ; Gram-positive bacteria ; health ; human development ; Humans ; identification ; insights ; intestinal microorganisms ; loci ; Lymphocytes B ; Male ; Metagenome ; Mice ; Mice, Inbred BALB C ; Microbial colonization ; Microbiota ; Microscopy ; Microscopy, Electron, Transmission ; Microscopy, Immunoelectron ; Molecular Sequence Data ; Multigene Family ; Mutation ; Neonates ; Pili ; polysaccharide ; proteins ; sequence analysis ; Sequence Homology, Amino Acid ; transcriptomics ; Transmission electron microscopy</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2011-07, Vol.108 (27), p.11217-11222</ispartof><rights>copyright © 1993–2008 National Academy of Sciences of the United States of America</rights><rights>Copyright National Academy of Sciences Jul 5, 2011</rights><rights>Wageningen University & Research</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c573t-e7bf5f1313b8debf63f6e021068728a88a11ca907139a5f92bf35b74552f6af3</citedby><cites>FETCH-LOGICAL-c573t-e7bf5f1313b8debf63f6e021068728a88a11ca907139a5f92bf35b74552f6af3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/108/27.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/27978769$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/27978769$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21690406$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>O'Connell Motherway, Mary</creatorcontrib><creatorcontrib>Zomer, Aldert</creatorcontrib><creatorcontrib>Leahy, Sinead C</creatorcontrib><creatorcontrib>Reunanen, Justus</creatorcontrib><creatorcontrib>Bottacini, Francesca</creatorcontrib><creatorcontrib>Claesson, Marcus J</creatorcontrib><creatorcontrib>O'Brien, Frances</creatorcontrib><creatorcontrib>Flynn, Kiera</creatorcontrib><creatorcontrib>Casey, Patrick G</creatorcontrib><creatorcontrib>Moreno Munoz, Jose Antonio</creatorcontrib><creatorcontrib>Kearney, Breda</creatorcontrib><creatorcontrib>Houston, Aileen M</creatorcontrib><creatorcontrib>O'Mahony, Caitlin</creatorcontrib><creatorcontrib>Higgins, Des G</creatorcontrib><creatorcontrib>Shanahan, Fergus</creatorcontrib><creatorcontrib>Palva, Airi</creatorcontrib><creatorcontrib>de Vos, Willem M</creatorcontrib><creatorcontrib>Fitzgerald, Gerald F</creatorcontrib><creatorcontrib>Ventura, Marco</creatorcontrib><creatorcontrib>O'Toole, Paul W</creatorcontrib><creatorcontrib>van Sinderen, Douwe</creatorcontrib><title>Functional genome analysis of Bifidobacterium breve UCC2003 reveals type IVb tight adherence (Tad) pili as an essential and conserved host-colonization factor</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Development of the human gut microbiota commences at birth, with bifidobacteria being among the first colonizers of the sterile newborn gastrointestinal tract. To date, the genetic basis of Bifidobacterium colonization and persistence remains poorly understood. Transcriptome analysis of the Bifidobacterium breve UCC2003 2.42-Mb genome in a murine colonization model revealed differential expression of a type IVb tight adherence (Tad) pilus-encoding gene cluster designated "tadââââ." Mutational analysis demonstrated that the tadââââ gene cluster is essential for efficient in vivo murine gut colonization, and immunogold transmission electron microscopy confirmed the presence of Tad pili at the poles of B. breve UCC2003 cells. Conservation of the Tad pilus-encoding locus among other B. breve strains and among sequenced Bifidobacterium genomes supports the notion of a ubiquitous pili-mediated host colonization and persistence mechanism for bifidobacteria.</description><subject>actinobacillus-actinomycetemcomitans</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - physiology</subject><subject>Base Sequence</subject><subject>Bifidobacterium</subject><subject>Bifidobacterium - genetics</subject><subject>Bifidobacterium - growth & development</subject><subject>Bifidobacterium - physiology</subject><subject>Bifidobacterium - ultrastructure</subject><subject>Bifidobacterium breve</subject><subject>Biological Sciences</subject><subject>Birth</subject><subject>Colonization</subject><subject>Comparative Genomic Hybridization</subject><subject>Conservation</subject><subject>Conserved sequence</subject><subject>Digestive tract</subject><subject>DNA, Bacterial - genetics</subject><subject>Female</subject><subject>fimbriae</subject><subject>Fimbriae, Bacterial - genetics</subject><subject>Fimbriae, Bacterial - physiology</subject><subject>Fimbriae, Bacterial - ultrastructure</subject><subject>formula-fed infants</subject><subject>gastrointestinal system</subject><subject>Gastrointestinal tract</subject><subject>Gastrointestinal Tract - microbiology</subject><subject>Gene clusters</subject><subject>Gene expression</subject><subject>gene expression regulation</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Genes</subject><subject>Genetic loci</subject><subject>Genome, Bacterial</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Germ-Free Life</subject><subject>Gram-positive bacteria</subject><subject>health</subject><subject>human development</subject><subject>Humans</subject><subject>identification</subject><subject>insights</subject><subject>intestinal microorganisms</subject><subject>loci</subject><subject>Lymphocytes B</subject><subject>Male</subject><subject>Metagenome</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microbial colonization</subject><subject>Microbiota</subject><subject>Microscopy</subject><subject>Microscopy, Electron, Transmission</subject><subject>Microscopy, Immunoelectron</subject><subject>Molecular Sequence Data</subject><subject>Multigene Family</subject><subject>Mutation</subject><subject>Neonates</subject><subject>Pili</subject><subject>polysaccharide</subject><subject>proteins</subject><subject>sequence analysis</subject><subject>Sequence Homology, Amino Acid</subject><subject>transcriptomics</subject><subject>Transmission electron 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Patrick G ; Moreno Munoz, Jose Antonio ; Kearney, Breda ; Houston, Aileen M ; O'Mahony, Caitlin ; Higgins, Des G ; Shanahan, Fergus ; Palva, Airi ; de Vos, Willem M ; Fitzgerald, Gerald F ; Ventura, Marco ; O'Toole, Paul W ; van Sinderen, Douwe</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c573t-e7bf5f1313b8debf63f6e021068728a88a11ca907139a5f92bf35b74552f6af3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>actinobacillus-actinomycetemcomitans</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - physiology</topic><topic>Base Sequence</topic><topic>Bifidobacterium</topic><topic>Bifidobacterium - genetics</topic><topic>Bifidobacterium - growth & development</topic><topic>Bifidobacterium - physiology</topic><topic>Bifidobacterium - ultrastructure</topic><topic>Bifidobacterium 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sterile newborn gastrointestinal tract. To date, the genetic basis of Bifidobacterium colonization and persistence remains poorly understood. Transcriptome analysis of the Bifidobacterium breve UCC2003 2.42-Mb genome in a murine colonization model revealed differential expression of a type IVb tight adherence (Tad) pilus-encoding gene cluster designated "tadââââ." Mutational analysis demonstrated that the tadââââ gene cluster is essential for efficient in vivo murine gut colonization, and immunogold transmission electron microscopy confirmed the presence of Tad pili at the poles of B. breve UCC2003 cells. Conservation of the Tad pilus-encoding locus among other B. breve strains and among sequenced Bifidobacterium genomes supports the notion of a ubiquitous pili-mediated host colonization and persistence mechanism for bifidobacteria.</abstract><cop>United States</cop><pub>National Academy of Sciences</pub><pmid>21690406</pmid><doi>10.1073/pnas.1105380108</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 2011-07, Vol.108 (27), p.11217-11222 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_pnas_primary_108_27_11217 |
| source | Jstor Complete Legacy; MEDLINE; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | actinobacillus-actinomycetemcomitans Amino Acid Sequence Animals Bacterial Proteins - genetics Bacterial Proteins - physiology Base Sequence Bifidobacterium Bifidobacterium - genetics Bifidobacterium - growth & development Bifidobacterium - physiology Bifidobacterium - ultrastructure Bifidobacterium breve Biological Sciences Birth Colonization Comparative Genomic Hybridization Conservation Conserved sequence Digestive tract DNA, Bacterial - genetics Female fimbriae Fimbriae, Bacterial - genetics Fimbriae, Bacterial - physiology Fimbriae, Bacterial - ultrastructure formula-fed infants gastrointestinal system Gastrointestinal tract Gastrointestinal Tract - microbiology Gene clusters Gene expression gene expression regulation Gene Expression Regulation, Bacterial Genes Genetic loci Genome, Bacterial Genomes Genomics Germ-Free Life Gram-positive bacteria health human development Humans identification insights intestinal microorganisms loci Lymphocytes B Male Metagenome Mice Mice, Inbred BALB C Microbial colonization Microbiota Microscopy Microscopy, Electron, Transmission Microscopy, Immunoelectron Molecular Sequence Data Multigene Family Mutation Neonates Pili polysaccharide proteins sequence analysis Sequence Homology, Amino Acid transcriptomics Transmission electron microscopy |
| title | Functional genome analysis of Bifidobacterium breve UCC2003 reveals type IVb tight adherence (Tad) pili as an essential and conserved host-colonization factor |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-08T03%3A05%3A58IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_pnas_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Functional%20genome%20analysis%20of%20Bifidobacterium%20breve%20UCC2003%20reveals%20type%20IVb%20tight%20adherence%20(Tad)%20pili%20as%20an%20essential%20and%20conserved%20host-colonization%20factor&rft.jtitle=Proceedings%20of%20the%20National%20Academy%20of%20Sciences%20-%20PNAS&rft.au=O'Connell%20Motherway,%20Mary&rft.date=2011-07-05&rft.volume=108&rft.issue=27&rft.spage=11217&rft.epage=11222&rft.pages=11217-11222&rft.issn=0027-8424&rft.eissn=1091-6490&rft_id=info:doi/10.1073/pnas.1105380108&rft_dat=%3Cjstor_pnas_%3E27978769%3C/jstor_pnas_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=875522914&rft_id=info:pmid/21690406&rft_jstor_id=27978769&rfr_iscdi=true |