Genetically encoded dendritic marker sheds light on neuronal connectivity in Drosophila
In recent years, Drosophila melanogaster has emerged as a powerful model for neuronal circuit development, pathology, and function. A major impediment to these studies has been the lack of a genetically encoded, specific, universal, and phenotypically neutral marker of the somatodendritic compartmen...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2010-11, Vol.107 (47), p.20553-20558 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Nicolaï, Laura J. J. Ramaekers, Ariane Raemaekers, Tim Drozdzecki, Andrzej Mauss, Alex S. Yan, Jiekun Landgraf, Matthias Annaert, Wim Hassan, Bassem A. Rubin, Gerald M. |
| description | In recent years, Drosophila melanogaster has emerged as a powerful model for neuronal circuit development, pathology, and function. A major impediment to these studies has been the lack of a genetically encoded, specific, universal, and phenotypically neutral marker of the somatodendritic compartment. We have developed such a marker and show that it is effective and specific in all neuronal populations tested in the peripheral and central nervous system. The marker, which we name DenMark (Dendritic Marker), is a hybrid protein of the mouse protein ICAM5/Telencephalin and the red fluorescent protein mCherry. We show that DenMark is a powerful tool for revealing novel aspects of the neuroanatomy of developing dendrites, identifying previously unknown dendritic arbors, and elucidating neuronal connectivity. |
| doi_str_mv | 10.1073/pnas.1010198107 |
| format | Article |
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J. ; Ramaekers, Ariane ; Raemaekers, Tim ; Drozdzecki, Andrzej ; Mauss, Alex S. ; Yan, Jiekun ; Landgraf, Matthias ; Annaert, Wim ; Hassan, Bassem A. ; Rubin, Gerald M.</creator><creatorcontrib>Nicolaï, Laura J. J. ; Ramaekers, Ariane ; Raemaekers, Tim ; Drozdzecki, Andrzej ; Mauss, Alex S. ; Yan, Jiekun ; Landgraf, Matthias ; Annaert, Wim ; Hassan, Bassem A. ; Rubin, Gerald M.</creatorcontrib><description>In recent years, Drosophila melanogaster has emerged as a powerful model for neuronal circuit development, pathology, and function. A major impediment to these studies has been the lack of a genetically encoded, specific, universal, and phenotypically neutral marker of the somatodendritic compartment. We have developed such a marker and show that it is effective and specific in all neuronal populations tested in the peripheral and central nervous system. The marker, which we name DenMark (Dendritic Marker), is a hybrid protein of the mouse protein ICAM5/Telencephalin and the red fluorescent protein mCherry. We show that DenMark is a powerful tool for revealing novel aspects of the neuroanatomy of developing dendrites, identifying previously unknown dendritic arbors, and elucidating neuronal connectivity.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.1010198107</identifier><identifier>PMID: 21059961</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>Anatomy ; Animals ; Axons ; Biological Sciences ; Biomarkers ; Brain ; Calyx ; Cell Adhesion Molecules - metabolism ; Central nervous system ; Circuits ; Dendrites ; Dendrites - genetics ; Drosophila ; Drosophila melanogaster ; Drosophila melanogaster - genetics ; Electroretinography ; Genetic Markers - genetics ; Genetics ; Genotype & phenotype ; Hippocampus - cytology ; Hybrids ; Immunohistochemistry ; Insects ; Instars ; Larvae ; Luminescent Proteins - genetics ; Luminescent Proteins - metabolism ; Membrane Glycoproteins - genetics ; Membrane Glycoproteins - metabolism ; Mice ; Microscopy, Confocal ; Mushroom bodies ; Nerve Tissue Proteins - genetics ; Nerve Tissue Proteins - metabolism ; Neural networks ; Neurons ; Neurons - cytology ; Neurosciences ; Pathology ; Proteins ; Recombinant Fusion Proteins - genetics ; Recombinant Fusion Proteins - metabolism ; Red Fluorescent Protein ; Synapses ; telencephalin</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2010-11, Vol.107 (47), p.20553-20558</ispartof><rights>Copyright National Academy of Sciences Nov 23, 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c489t-e5448c1b06a22d36b8ad58dec94e617145e263abd8edfe91d39cb244a625e2083</citedby><cites>FETCH-LOGICAL-c489t-e5448c1b06a22d36b8ad58dec94e617145e263abd8edfe91d39cb244a625e2083</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/107/47.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/25756735$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/25756735$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,725,778,782,801,883,27907,27908,53774,53776,58000,58233</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21059961$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nicolaï, Laura J. J.</creatorcontrib><creatorcontrib>Ramaekers, Ariane</creatorcontrib><creatorcontrib>Raemaekers, Tim</creatorcontrib><creatorcontrib>Drozdzecki, Andrzej</creatorcontrib><creatorcontrib>Mauss, Alex S.</creatorcontrib><creatorcontrib>Yan, Jiekun</creatorcontrib><creatorcontrib>Landgraf, Matthias</creatorcontrib><creatorcontrib>Annaert, Wim</creatorcontrib><creatorcontrib>Hassan, Bassem A.</creatorcontrib><creatorcontrib>Rubin, Gerald M.</creatorcontrib><title>Genetically encoded dendritic marker sheds light on neuronal connectivity in Drosophila</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>In recent years, Drosophila melanogaster has emerged as a powerful model for neuronal circuit development, pathology, and function. A major impediment to these studies has been the lack of a genetically encoded, specific, universal, and phenotypically neutral marker of the somatodendritic compartment. 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We show that DenMark is a powerful tool for revealing novel aspects of the neuroanatomy of developing dendrites, identifying previously unknown dendritic arbors, and elucidating neuronal connectivity.</description><subject>Anatomy</subject><subject>Animals</subject><subject>Axons</subject><subject>Biological Sciences</subject><subject>Biomarkers</subject><subject>Brain</subject><subject>Calyx</subject><subject>Cell Adhesion Molecules - metabolism</subject><subject>Central nervous system</subject><subject>Circuits</subject><subject>Dendrites</subject><subject>Dendrites - genetics</subject><subject>Drosophila</subject><subject>Drosophila melanogaster</subject><subject>Drosophila melanogaster - genetics</subject><subject>Electroretinography</subject><subject>Genetic Markers - genetics</subject><subject>Genetics</subject><subject>Genotype & phenotype</subject><subject>Hippocampus - cytology</subject><subject>Hybrids</subject><subject>Immunohistochemistry</subject><subject>Insects</subject><subject>Instars</subject><subject>Larvae</subject><subject>Luminescent Proteins - genetics</subject><subject>Luminescent Proteins - metabolism</subject><subject>Membrane Glycoproteins - genetics</subject><subject>Membrane Glycoproteins - metabolism</subject><subject>Mice</subject><subject>Microscopy, Confocal</subject><subject>Mushroom bodies</subject><subject>Nerve Tissue Proteins - genetics</subject><subject>Nerve Tissue Proteins - metabolism</subject><subject>Neural networks</subject><subject>Neurons</subject><subject>Neurons - cytology</subject><subject>Neurosciences</subject><subject>Pathology</subject><subject>Proteins</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>Red Fluorescent Protein</subject><subject>Synapses</subject><subject>telencephalin</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkk1vEzEQhi0EoqFw5gSyuHBa6u-1L0hVgYJUiQuIo-XYk8ZhYwd7t1L-PV4lNMCl8sHWzDPvfHgQeknJO0p6frFLrrZXO0Y3wyO0oMTQTglDHqMFIazvtGDiDD2rdUMIMVKTp-iMUSKNUXSBflxDgjF6Nwx7DMnnAAEHSKHEZsVbV35CwXUNoeIh3q5HnBNOMJWc3IB9Tgn8GO_iuMcx4Q8l17xbx8E9R09Wbqjw4nifo--fPn67-tzdfL3-cnV503mhzdiBFEJ7uiTKMRa4WmoXpA7gjQBFeyokMMXdMmgIKzA0cOOXTAinWPMQzc_R-4PublpuIXhIY3GD3ZXYSt_b7KL915Pi2t7mO8ta_02_Cbw9CpT8a4I62m2sHobBJchTtVpSoYTg6mGSG2W4lvphkjLKBdWkkW_-Izd5Km20M9SSGtHPiS8OkG_jrQVW9-1RYuc1sPMa2NMatIjXf0_lnv_z7w3AR2COPMn1VvSWESl5Q14dkE0dczlJyF6qnkv-G5LZw0s</recordid><startdate>20101123</startdate><enddate>20101123</enddate><creator>Nicolaï, Laura J. J.</creator><creator>Ramaekers, Ariane</creator><creator>Raemaekers, Tim</creator><creator>Drozdzecki, Andrzej</creator><creator>Mauss, Alex S.</creator><creator>Yan, Jiekun</creator><creator>Landgraf, Matthias</creator><creator>Annaert, Wim</creator><creator>Hassan, Bassem A.</creator><creator>Rubin, Gerald M.</creator><general>National Academy of Sciences</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20101123</creationdate><title>Genetically encoded dendritic marker sheds light on neuronal connectivity in Drosophila</title><author>Nicolaï, Laura J. J. ; Ramaekers, Ariane ; Raemaekers, Tim ; Drozdzecki, Andrzej ; Mauss, Alex S. ; Yan, Jiekun ; Landgraf, Matthias ; Annaert, Wim ; Hassan, Bassem A. ; Rubin, Gerald M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c489t-e5448c1b06a22d36b8ad58dec94e617145e263abd8edfe91d39cb244a625e2083</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Anatomy</topic><topic>Animals</topic><topic>Axons</topic><topic>Biological Sciences</topic><topic>Biomarkers</topic><topic>Brain</topic><topic>Calyx</topic><topic>Cell Adhesion Molecules - metabolism</topic><topic>Central nervous system</topic><topic>Circuits</topic><topic>Dendrites</topic><topic>Dendrites - genetics</topic><topic>Drosophila</topic><topic>Drosophila melanogaster</topic><topic>Drosophila melanogaster - genetics</topic><topic>Electroretinography</topic><topic>Genetic Markers - genetics</topic><topic>Genetics</topic><topic>Genotype & phenotype</topic><topic>Hippocampus - cytology</topic><topic>Hybrids</topic><topic>Immunohistochemistry</topic><topic>Insects</topic><topic>Instars</topic><topic>Larvae</topic><topic>Luminescent Proteins - genetics</topic><topic>Luminescent Proteins - metabolism</topic><topic>Membrane Glycoproteins - genetics</topic><topic>Membrane Glycoproteins - metabolism</topic><topic>Mice</topic><topic>Microscopy, Confocal</topic><topic>Mushroom bodies</topic><topic>Nerve Tissue Proteins - genetics</topic><topic>Nerve Tissue Proteins - metabolism</topic><topic>Neural networks</topic><topic>Neurons</topic><topic>Neurons - cytology</topic><topic>Neurosciences</topic><topic>Pathology</topic><topic>Proteins</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Recombinant Fusion Proteins - metabolism</topic><topic>Red Fluorescent Protein</topic><topic>Synapses</topic><topic>telencephalin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nicolaï, Laura J. 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J.</au><au>Ramaekers, Ariane</au><au>Raemaekers, Tim</au><au>Drozdzecki, Andrzej</au><au>Mauss, Alex S.</au><au>Yan, Jiekun</au><au>Landgraf, Matthias</au><au>Annaert, Wim</au><au>Hassan, Bassem A.</au><au>Rubin, Gerald M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetically encoded dendritic marker sheds light on neuronal connectivity in Drosophila</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>2010-11-23</date><risdate>2010</risdate><volume>107</volume><issue>47</issue><spage>20553</spage><epage>20558</epage><pages>20553-20558</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>In recent years, Drosophila melanogaster has emerged as a powerful model for neuronal circuit development, pathology, and function. A major impediment to these studies has been the lack of a genetically encoded, specific, universal, and phenotypically neutral marker of the somatodendritic compartment. We have developed such a marker and show that it is effective and specific in all neuronal populations tested in the peripheral and central nervous system. The marker, which we name DenMark (Dendritic Marker), is a hybrid protein of the mouse protein ICAM5/Telencephalin and the red fluorescent protein mCherry. We show that DenMark is a powerful tool for revealing novel aspects of the neuroanatomy of developing dendrites, identifying previously unknown dendritic arbors, and elucidating neuronal connectivity.</abstract><cop>United States</cop><pub>National Academy of Sciences</pub><pmid>21059961</pmid><doi>10.1073/pnas.1010198107</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Anatomy Animals Axons Biological Sciences Biomarkers Brain Calyx Cell Adhesion Molecules - metabolism Central nervous system Circuits Dendrites Dendrites - genetics Drosophila Drosophila melanogaster Drosophila melanogaster - genetics Electroretinography Genetic Markers - genetics Genetics Genotype & phenotype Hippocampus - cytology Hybrids Immunohistochemistry Insects Instars Larvae Luminescent Proteins - genetics Luminescent Proteins - metabolism Membrane Glycoproteins - genetics Membrane Glycoproteins - metabolism Mice Microscopy, Confocal Mushroom bodies Nerve Tissue Proteins - genetics Nerve Tissue Proteins - metabolism Neural networks Neurons Neurons - cytology Neurosciences Pathology Proteins Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Red Fluorescent Protein Synapses telencephalin |
| title | Genetically encoded dendritic marker sheds light on neuronal connectivity in Drosophila |
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