Droplet microfluidic technology for single-cell high-throughput screening

We present a droplet-based microfluidic technology that enables high-throughput screening of single mammalian cells. This integrated platform allows for the encapsulation of single cells and reagents in independent aqueous microdroplets (1 pL to 10 nL volumes) dispersed in an immiscible carrier oil...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 2009-08, Vol.106 (34), p.14195-14200
Hauptverfasser:	Brouzes, Eric, Medkova, Martina, Savenelli, Neal, Marran, Dave, Twardowski, Mariusz, Hutchison, J. Brian, Rothberg, Jonathan M, Link, Darren R, Perrimon, Norbert, Samuels, Michael L
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological Sciences Biomedical technology Cell growth Cell Survival - drug effects Cells Cytotoxicity Drug Evaluation, Preclinical - methods Dyes Emulsions Encapsulation Fluorescent Dyes - chemistry Humans Libraries Mammals Microfluidic Analytical Techniques - methods Microfluidics - instrumentation Microfluidics - methods Mitomycin - chemistry Mitomycin - pharmacology Nozzles Physical Sciences Preclinical drug evaluation Reproducibility of Results Time Factors Toxicity U937 Cells Validation studies Viability
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