family of thermostable fungal cellulases created by structure-guided recombination

SCHEMA structure-guided recombination of 3 fungal class II cellobiohydrolases (CBH II cellulases) has yielded a collection of highly thermostable CBH II chimeras. Twenty-three of 48 genes sampled from the 6,561 possible chimeric sequences were secreted by the Saccharomyces cerevisiae heterologous ho...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 2009-04, Vol.106 (14), p.5610-5615
Hauptverfasser:	Heinzelman, Pete, Snow, Christopher D, Wu, Indira, Nguyen, Catherine, Villalobos, Alan, Govindarajan, Sridhar, Minshull, Jeremy, Arnold, Frances H
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetates Adenylate cyclase Amino acid sequence Amino acids Biochemistry Biological Sciences Biomass Catalysis cellobiohydrolase Cellulase cellulases Cellulases - genetics Cellulose cellulose 1,4-beta-cellobiosidase Chimeras enzymatic hydrolysis enzyme activity Enzyme Stability Enzymes Fungal Proteins - genetics Gels gene expression Genes genetic engineering genetic recombination genetically engineered microorganisms Genetics half life Half lives heat inactivation heat stability Hot Temperature Humicola Humicola insolens Hydrolysis inactivation Inventories Libraries Mathematical models pH effects phosphoric acid Protein Engineering - methods Recombinant Fusion Proteins Recombination Recombination, Genetic Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Sodium Sugars Temperature Temperature effects Thermal stability thermophilic fungi Yeast
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