Caenorhabditis elegans vulva: A post-embryonic gene regulatory network controlling organogenesis
The Caenorhabditis elegans vulva is an elegant model for dissecting a gene regulatory network (GRN) that directs postembryonic organogenesis. The mature vulva comprises seven cell types (vulA, vulB1, vulB2, vulC, vulD, vulE, and vulF), each with its own unique pattern of spatial and temporal gene ex...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2008-12, Vol.105 (51), p.20095-20099 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Ririe, Ted O Fernandes, Jolene S Sternberg, Paul W |
| description | The Caenorhabditis elegans vulva is an elegant model for dissecting a gene regulatory network (GRN) that directs postembryonic organogenesis. The mature vulva comprises seven cell types (vulA, vulB1, vulB2, vulC, vulD, vulE, and vulF), each with its own unique pattern of spatial and temporal gene expression. The mechanisms that specify these cell types in a precise spatial pattern are not well understood. Using reverse genetic screens, we identified novel components of the vulval GRN, including nhr-113 in vulA. Several transcription factors (lin-11, lin-29, cog-1, egl-38, and nhr-67) interact with each other and act in concert to regulate target gene expression in the diverse vulval cell types. For example, egl-38 (Pax2/5/8) stabilizes the vulF fate by positively regulating vulF characteristics and by inhibiting characteristics associated with the neighboring vulE cells. nhr-67 and egl-38 regulate cog-1, helping restrict its expression to vulE. Computational approaches have been successfully used to identify functional cis-regulatory motifs in the zmp-1 (zinc metalloproteinase) promoter. These results provide an overview of the regulatory network architecture for each vulval cell type. |
| doi_str_mv | 10.1073/pnas.0806377105 |
| format | Article |
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The mature vulva comprises seven cell types (vulA, vulB1, vulB2, vulC, vulD, vulE, and vulF), each with its own unique pattern of spatial and temporal gene expression. The mechanisms that specify these cell types in a precise spatial pattern are not well understood. Using reverse genetic screens, we identified novel components of the vulval GRN, including nhr-113 in vulA. Several transcription factors (lin-11, lin-29, cog-1, egl-38, and nhr-67) interact with each other and act in concert to regulate target gene expression in the diverse vulval cell types. For example, egl-38 (Pax2/5/8) stabilizes the vulF fate by positively regulating vulF characteristics and by inhibiting characteristics associated with the neighboring vulE cells. nhr-67 and egl-38 regulate cog-1, helping restrict its expression to vulE. Computational approaches have been successfully used to identify functional cis-regulatory motifs in the zmp-1 (zinc metalloproteinase) promoter. These results provide an overview of the regulatory network architecture for each vulval cell type.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.0806377105</identifier><identifier>PMID: 19104047</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>Animals ; Biological Sciences ; Caenorhabditis elegans ; Caenorhabditis elegans - embryology ; Caenorhabditis elegans - genetics ; Cell lines ; Cells ; Cellular differentiation ; Chromium ; Computer applications ; Embryo, Nonmammalian ; Embryology ; Embryonic Induction ; Enhancer Elements, Genetic ; Female ; Gene expression ; Gene Networks in Development and Evolution Special Feature Sackler Colloquium ; Gene Regulatory Networks - physiology ; Genes ; Genes, Helminth ; Genetic screening ; Genetic testing ; Metalloproteases - genetics ; Morphogenesis ; Nematodes ; Organogenesis ; Organogenesis - genetics ; Pax2 protein ; Promoters ; Reviews ; Transcription Factors ; Vulva ; Vulva - embryology ; Vulva - growth & development ; zinc metalloproteinase</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2008-12, Vol.105 (51), p.20095-20099</ispartof><rights>Copyright 2008 The National Academy of Sciences of the United States of America</rights><rights>Copyright National Academy of Sciences Dec 23, 2008</rights><rights>2008 by The National Academy of Sciences of the USA</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c554t-2f3409dbeaec9b8dd6f135420ee80b9eb7f98a267d7dba0827307e7d86fe42d93</citedby><cites>FETCH-LOGICAL-c554t-2f3409dbeaec9b8dd6f135420ee80b9eb7f98a267d7dba0827307e7d86fe42d93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/105/51.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/25465784$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/25465784$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27922,27923,53789,53791,58015,58248</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19104047$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ririe, Ted O</creatorcontrib><creatorcontrib>Fernandes, Jolene S</creatorcontrib><creatorcontrib>Sternberg, Paul W</creatorcontrib><title>Caenorhabditis elegans vulva: A post-embryonic gene regulatory network controlling organogenesis</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The Caenorhabditis elegans vulva is an elegant model for dissecting a gene regulatory network (GRN) that directs postembryonic organogenesis. The mature vulva comprises seven cell types (vulA, vulB1, vulB2, vulC, vulD, vulE, and vulF), each with its own unique pattern of spatial and temporal gene expression. The mechanisms that specify these cell types in a precise spatial pattern are not well understood. Using reverse genetic screens, we identified novel components of the vulval GRN, including nhr-113 in vulA. Several transcription factors (lin-11, lin-29, cog-1, egl-38, and nhr-67) interact with each other and act in concert to regulate target gene expression in the diverse vulval cell types. For example, egl-38 (Pax2/5/8) stabilizes the vulF fate by positively regulating vulF characteristics and by inhibiting characteristics associated with the neighboring vulE cells. nhr-67 and egl-38 regulate cog-1, helping restrict its expression to vulE. Computational approaches have been successfully used to identify functional cis-regulatory motifs in the zmp-1 (zinc metalloproteinase) promoter. These results provide an overview of the regulatory network architecture for each vulval cell type.</description><subject>Animals</subject><subject>Biological Sciences</subject><subject>Caenorhabditis elegans</subject><subject>Caenorhabditis elegans - embryology</subject><subject>Caenorhabditis elegans - genetics</subject><subject>Cell lines</subject><subject>Cells</subject><subject>Cellular differentiation</subject><subject>Chromium</subject><subject>Computer applications</subject><subject>Embryo, Nonmammalian</subject><subject>Embryology</subject><subject>Embryonic Induction</subject><subject>Enhancer Elements, Genetic</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene Networks in Development and Evolution Special Feature Sackler Colloquium</subject><subject>Gene Regulatory Networks - physiology</subject><subject>Genes</subject><subject>Genes, Helminth</subject><subject>Genetic screening</subject><subject>Genetic testing</subject><subject>Metalloproteases - genetics</subject><subject>Morphogenesis</subject><subject>Nematodes</subject><subject>Organogenesis</subject><subject>Organogenesis - genetics</subject><subject>Pax2 protein</subject><subject>Promoters</subject><subject>Reviews</subject><subject>Transcription Factors</subject><subject>Vulva</subject><subject>Vulva - embryology</subject><subject>Vulva - growth & development</subject><subject>zinc metalloproteinase</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0r1v1DAYBvAIgei1MDMBEQMSQ9rXjh3HDEjViS-pEgN0Nk7yJvXhs692cvT-exzdqQcsnTK8Pz-x_TjLXhA4JyDKi43T8RxqqEohCPBH2YKAJEXFJDzOFgBUFDWj7CQ7jXEFAJLX8DQ7IZIAAyYW2c-lRufDjW46M5qYo8VBu5hvJ7vV7_PLfOPjWOC6CTvvTJsP6DAPOExWjz7scofjbx9-5a13Y_DWGjfkPqQIP8to4rPsSa9txOeH71l2_enjj-WX4urb56_Ly6ui5ZyNBe1LBrJrUGMrm7rrqp6UnFFArKGR2Ihe1ppWohNdo6GmogSBoqurHhntZHmWfdjnbqZmjV2LaT_aqk0wax12ymuj_p04c6MGv1W0opJykgLeHgKCv50wjmptYovWaod-iqqSkpJ0yw9CChTqqiwTfPMfXPkpuHQLyZCSccohoYs9aoOPMWB_v2UCau5YzR2rY8dpxau_T3r0h1ITeHcA88pjHFecpD-nR6D6ydoR78Zk8wdsIi_3ZBVT5feGclZxUbM0f72f99orPQQT1fX3-YBAuChn8QciJ9CV</recordid><startdate>20081223</startdate><enddate>20081223</enddate><creator>Ririe, Ted O</creator><creator>Fernandes, Jolene S</creator><creator>Sternberg, Paul W</creator><general>National Academy of Sciences</general><general>National Acad Sciences</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20081223</creationdate><title>Caenorhabditis elegans vulva: A post-embryonic gene regulatory network controlling organogenesis</title><author>Ririe, Ted O ; Fernandes, Jolene S ; Sternberg, Paul W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c554t-2f3409dbeaec9b8dd6f135420ee80b9eb7f98a267d7dba0827307e7d86fe42d93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Biological Sciences</topic><topic>Caenorhabditis elegans</topic><topic>Caenorhabditis elegans - embryology</topic><topic>Caenorhabditis elegans - genetics</topic><topic>Cell lines</topic><topic>Cells</topic><topic>Cellular differentiation</topic><topic>Chromium</topic><topic>Computer applications</topic><topic>Embryo, Nonmammalian</topic><topic>Embryology</topic><topic>Embryonic Induction</topic><topic>Enhancer Elements, Genetic</topic><topic>Female</topic><topic>Gene expression</topic><topic>Gene Networks in Development and Evolution Special Feature Sackler Colloquium</topic><topic>Gene Regulatory Networks - physiology</topic><topic>Genes</topic><topic>Genes, Helminth</topic><topic>Genetic screening</topic><topic>Genetic testing</topic><topic>Metalloproteases - genetics</topic><topic>Morphogenesis</topic><topic>Nematodes</topic><topic>Organogenesis</topic><topic>Organogenesis - genetics</topic><topic>Pax2 protein</topic><topic>Promoters</topic><topic>Reviews</topic><topic>Transcription Factors</topic><topic>Vulva</topic><topic>Vulva - embryology</topic><topic>Vulva - growth & development</topic><topic>zinc metalloproteinase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ririe, Ted O</creatorcontrib><creatorcontrib>Fernandes, Jolene S</creatorcontrib><creatorcontrib>Sternberg, Paul W</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ririe, Ted O</au><au>Fernandes, Jolene S</au><au>Sternberg, Paul W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Caenorhabditis elegans vulva: A post-embryonic gene regulatory network controlling organogenesis</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>2008-12-23</date><risdate>2008</risdate><volume>105</volume><issue>51</issue><spage>20095</spage><epage>20099</epage><pages>20095-20099</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>The Caenorhabditis elegans vulva is an elegant model for dissecting a gene regulatory network (GRN) that directs postembryonic organogenesis. The mature vulva comprises seven cell types (vulA, vulB1, vulB2, vulC, vulD, vulE, and vulF), each with its own unique pattern of spatial and temporal gene expression. The mechanisms that specify these cell types in a precise spatial pattern are not well understood. Using reverse genetic screens, we identified novel components of the vulval GRN, including nhr-113 in vulA. Several transcription factors (lin-11, lin-29, cog-1, egl-38, and nhr-67) interact with each other and act in concert to regulate target gene expression in the diverse vulval cell types. For example, egl-38 (Pax2/5/8) stabilizes the vulF fate by positively regulating vulF characteristics and by inhibiting characteristics associated with the neighboring vulE cells. nhr-67 and egl-38 regulate cog-1, helping restrict its expression to vulE. Computational approaches have been successfully used to identify functional cis-regulatory motifs in the zmp-1 (zinc metalloproteinase) promoter. These results provide an overview of the regulatory network architecture for each vulval cell type.</abstract><cop>United States</cop><pub>National Academy of Sciences</pub><pmid>19104047</pmid><doi>10.1073/pnas.0806377105</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Biological Sciences Caenorhabditis elegans Caenorhabditis elegans - embryology Caenorhabditis elegans - genetics Cell lines Cells Cellular differentiation Chromium Computer applications Embryo, Nonmammalian Embryology Embryonic Induction Enhancer Elements, Genetic Female Gene expression Gene Networks in Development and Evolution Special Feature Sackler Colloquium Gene Regulatory Networks - physiology Genes Genes, Helminth Genetic screening Genetic testing Metalloproteases - genetics Morphogenesis Nematodes Organogenesis Organogenesis - genetics Pax2 protein Promoters Reviews Transcription Factors Vulva Vulva - embryology Vulva - growth & development zinc metalloproteinase |
| title | Caenorhabditis elegans vulva: A post-embryonic gene regulatory network controlling organogenesis |
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