Transmission of Y chromosomes from XY female mice was made possible by the replacement of cytoplasm during oocyte maturation
The B6.YTIR sex-reversed female mouse is anatomically normal at young ages but fails to produce offspring. We have previously shown that its oocytes go through the meiotic cell cycle up to the second metaphase; however, the meiotic spindle is not properly organized, the second meiotic division goes...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2008-09, Vol.105 (37), p.13918-13923 |
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| creator | Obata, Yayoi Villemure, Michele Kono, Tomohiro Taketo, Teruko |
| description | The B6.YTIR sex-reversed female mouse is anatomically normal at young ages but fails to produce offspring. We have previously shown that its oocytes go through the meiotic cell cycle up to the second metaphase; however, the meiotic spindle is not properly organized, the second meiotic division goes awry after activation or fertilization, and none of the oocytes initiate embryonic development. In the present study, we transferred the nuclei of GV-stage oocytes from XY females into the enucleated GV-stage oocytes from (B6.DBA)F1.XX females. The resultant reconstructed oocytes properly assembled second meiotic spindles after in vitro maturation and produced healthy offspring after in vitro fertilization. Some male pups inherited maternal Y chromosomes. We conclude that the cytoplasm of the XY oocyte is insufficient to support spindle formation at the second metaphase whereas its replacement with the cytoplasmic material from an XX oocyte allows normal development. |
| doi_str_mv | 10.1073/pnas.0802680105 |
| format | Article |
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We have previously shown that its oocytes go through the meiotic cell cycle up to the second metaphase; however, the meiotic spindle is not properly organized, the second meiotic division goes awry after activation or fertilization, and none of the oocytes initiate embryonic development. In the present study, we transferred the nuclei of GV-stage oocytes from XY females into the enucleated GV-stage oocytes from (B6.DBA)F1.XX females. The resultant reconstructed oocytes properly assembled second meiotic spindles after in vitro maturation and produced healthy offspring after in vitro fertilization. Some male pups inherited maternal Y chromosomes. We conclude that the cytoplasm of the XY oocyte is insufficient to support spindle formation at the second metaphase whereas its replacement with the cytoplasmic material from an XX oocyte allows normal development.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.0802680105</identifier><identifier>PMID: 18772381</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>Age ; Animal reproduction ; Animals ; Biological Sciences ; Cell cycle ; Cell Differentiation ; Cell division ; Cells, Cultured ; Chromosomes ; Control groups ; Cytoplasm ; Cytoplasm - metabolism ; DNA-Binding Proteins - genetics ; Embryogenesis ; Female ; Fertilization ; Karyotype ; Karyotyping ; Male ; Meiosis ; Metaphase ; Mice ; Microtubules ; Mitotic spindle apparatus ; Nuclei ; Oocytes ; Oocytes - cytology ; Oocytes - metabolism ; Polymorphism, Genetic - genetics ; Reproductive technologies ; Rodents ; Sex chromosomes ; Spindle Apparatus - genetics ; Spindles ; Transcription Factors - genetics ; X Chromosome - genetics ; Y chromosome ; Y Chromosome - genetics</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2008-09, Vol.105 (37), p.13918-13923</ispartof><rights>Copyright 2008 The National Academy of Sciences of the United States of America</rights><rights>Copyright National Academy of Sciences Sep 16, 2008</rights><rights>2008 by The National Academy of Sciences of the USA</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c620t-220982cd2f24f8a189002a64de6d5b2ebeaed9a784fbce22f2eda7158b785f783</citedby><cites>FETCH-LOGICAL-c620t-220982cd2f24f8a189002a64de6d5b2ebeaed9a784fbce22f2eda7158b785f783</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/105/37.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/25464151$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/25464151$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27903,27904,53769,53771,57995,58228</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18772381$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Obata, Yayoi</creatorcontrib><creatorcontrib>Villemure, Michele</creatorcontrib><creatorcontrib>Kono, Tomohiro</creatorcontrib><creatorcontrib>Taketo, Teruko</creatorcontrib><title>Transmission of Y chromosomes from XY female mice was made possible by the replacement of cytoplasm during oocyte maturation</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The B6.YTIR sex-reversed female mouse is anatomically normal at young ages but fails to produce offspring. We have previously shown that its oocytes go through the meiotic cell cycle up to the second metaphase; however, the meiotic spindle is not properly organized, the second meiotic division goes awry after activation or fertilization, and none of the oocytes initiate embryonic development. In the present study, we transferred the nuclei of GV-stage oocytes from XY females into the enucleated GV-stage oocytes from (B6.DBA)F1.XX females. The resultant reconstructed oocytes properly assembled second meiotic spindles after in vitro maturation and produced healthy offspring after in vitro fertilization. Some male pups inherited maternal Y chromosomes. We conclude that the cytoplasm of the XY oocyte is insufficient to support spindle formation at the second metaphase whereas its replacement with the cytoplasmic material from an XX oocyte allows normal development.</description><subject>Age</subject><subject>Animal reproduction</subject><subject>Animals</subject><subject>Biological Sciences</subject><subject>Cell cycle</subject><subject>Cell Differentiation</subject><subject>Cell division</subject><subject>Cells, Cultured</subject><subject>Chromosomes</subject><subject>Control groups</subject><subject>Cytoplasm</subject><subject>Cytoplasm - metabolism</subject><subject>DNA-Binding Proteins - genetics</subject><subject>Embryogenesis</subject><subject>Female</subject><subject>Fertilization</subject><subject>Karyotype</subject><subject>Karyotyping</subject><subject>Male</subject><subject>Meiosis</subject><subject>Metaphase</subject><subject>Mice</subject><subject>Microtubules</subject><subject>Mitotic spindle apparatus</subject><subject>Nuclei</subject><subject>Oocytes</subject><subject>Oocytes - cytology</subject><subject>Oocytes - metabolism</subject><subject>Polymorphism, Genetic - genetics</subject><subject>Reproductive technologies</subject><subject>Rodents</subject><subject>Sex chromosomes</subject><subject>Spindle Apparatus - genetics</subject><subject>Spindles</subject><subject>Transcription Factors - genetics</subject><subject>X Chromosome - genetics</subject><subject>Y chromosome</subject><subject>Y Chromosome - genetics</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhiMEokvhzAmwOCBxSGs7duJckFDFl1SJA61ET5bjjHeziuPUToCV-PFMtKsucOnJ1swz73y8Wfac0TNGq-J8HEw6o4ryUlFG5YNsxWjN8lLU9GG2opRXuRJcnGRPUtpSSmup6OPshKmq4oViq-z3VTRD8l1KXRhIcOSG2E0MPqTgIRGHX_L9hjjwpgfiOwvkp0nEmxbIGLCqwXCzI9MGSISxNxY8DNOiZHdTwEDypJ1jN6xJCBhCETPN0UzY72n2yJk-wbPDe5pdf_xwdfE5v_z66cvF-8vclpxOOee0Vty23HHhlGGqxsVMKVooW9lwaMBAW5tKCddY4IhBayomVVMp6SpVnGbv9rrj3HhoLQ4YTa_H2HkTdzqYTv-bGbqNXocfmkshpBQo8OYgEMPtDGnSeDELfW8GCHPSZS1lUZbyXpBTzkqcCsHX_4HbMMcBr4AMKxTH8RE630M24qkjuLuRGdWL_3rxXx_9x4qXf2965A-GI0AOwFJ5lJO6qDQrarZ0fXsPot3c9xP8mpB9sWe3aQrxDsa7lYLJpd2rfd6ZoM06dklff1sWpEwKWihR_AEiT9ox</recordid><startdate>20080916</startdate><enddate>20080916</enddate><creator>Obata, Yayoi</creator><creator>Villemure, Michele</creator><creator>Kono, Tomohiro</creator><creator>Taketo, Teruko</creator><general>National Academy of Sciences</general><general>National Acad Sciences</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20080916</creationdate><title>Transmission of Y chromosomes from XY female mice was made possible by the replacement of cytoplasm during oocyte maturation</title><author>Obata, Yayoi ; Villemure, Michele ; Kono, Tomohiro ; Taketo, Teruko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c620t-220982cd2f24f8a189002a64de6d5b2ebeaed9a784fbce22f2eda7158b785f783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Age</topic><topic>Animal reproduction</topic><topic>Animals</topic><topic>Biological Sciences</topic><topic>Cell cycle</topic><topic>Cell Differentiation</topic><topic>Cell division</topic><topic>Cells, Cultured</topic><topic>Chromosomes</topic><topic>Control groups</topic><topic>Cytoplasm</topic><topic>Cytoplasm - metabolism</topic><topic>DNA-Binding Proteins - genetics</topic><topic>Embryogenesis</topic><topic>Female</topic><topic>Fertilization</topic><topic>Karyotype</topic><topic>Karyotyping</topic><topic>Male</topic><topic>Meiosis</topic><topic>Metaphase</topic><topic>Mice</topic><topic>Microtubules</topic><topic>Mitotic spindle apparatus</topic><topic>Nuclei</topic><topic>Oocytes</topic><topic>Oocytes - cytology</topic><topic>Oocytes - metabolism</topic><topic>Polymorphism, Genetic - genetics</topic><topic>Reproductive technologies</topic><topic>Rodents</topic><topic>Sex chromosomes</topic><topic>Spindle Apparatus - genetics</topic><topic>Spindles</topic><topic>Transcription Factors - genetics</topic><topic>X Chromosome - genetics</topic><topic>Y chromosome</topic><topic>Y Chromosome - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Obata, Yayoi</creatorcontrib><creatorcontrib>Villemure, Michele</creatorcontrib><creatorcontrib>Kono, Tomohiro</creatorcontrib><creatorcontrib>Taketo, Teruko</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Obata, Yayoi</au><au>Villemure, Michele</au><au>Kono, Tomohiro</au><au>Taketo, Teruko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transmission of Y chromosomes from XY female mice was made possible by the replacement of cytoplasm during oocyte maturation</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>2008-09-16</date><risdate>2008</risdate><volume>105</volume><issue>37</issue><spage>13918</spage><epage>13923</epage><pages>13918-13923</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>The B6.YTIR sex-reversed female mouse is anatomically normal at young ages but fails to produce offspring. We have previously shown that its oocytes go through the meiotic cell cycle up to the second metaphase; however, the meiotic spindle is not properly organized, the second meiotic division goes awry after activation or fertilization, and none of the oocytes initiate embryonic development. In the present study, we transferred the nuclei of GV-stage oocytes from XY females into the enucleated GV-stage oocytes from (B6.DBA)F1.XX females. The resultant reconstructed oocytes properly assembled second meiotic spindles after in vitro maturation and produced healthy offspring after in vitro fertilization. Some male pups inherited maternal Y chromosomes. 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| subjects | Age Animal reproduction Animals Biological Sciences Cell cycle Cell Differentiation Cell division Cells, Cultured Chromosomes Control groups Cytoplasm Cytoplasm - metabolism DNA-Binding Proteins - genetics Embryogenesis Female Fertilization Karyotype Karyotyping Male Meiosis Metaphase Mice Microtubules Mitotic spindle apparatus Nuclei Oocytes Oocytes - cytology Oocytes - metabolism Polymorphism, Genetic - genetics Reproductive technologies Rodents Sex chromosomes Spindle Apparatus - genetics Spindles Transcription Factors - genetics X Chromosome - genetics Y chromosome Y Chromosome - genetics |
| title | Transmission of Y chromosomes from XY female mice was made possible by the replacement of cytoplasm during oocyte maturation |
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