honey bee odorant receptor for the queen substance 9-oxo-2-decenoic acid
By using a functional genomics approach, we have identified a honey bee [Apis mellifera (Am)] odorant receptor (Or) for the queen substance 9-oxo-2-decenoic acid (9-ODA). Honey bees live in large eusocial colonies in which a single queen is responsible for reproduction, several thousand sterile fema...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2007-09, Vol.104 (36), p.14383-14388 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Wanner, Kevin W Nichols, Andrew S Walden, Kimberly K.O Brockmann, Axel Luetje, Charles W Robertson, Hugh M |
| description | By using a functional genomics approach, we have identified a honey bee [Apis mellifera (Am)] odorant receptor (Or) for the queen substance 9-oxo-2-decenoic acid (9-ODA). Honey bees live in large eusocial colonies in which a single queen is responsible for reproduction, several thousand sterile female worker bees complete a myriad of tasks to maintain the colony, and several hundred male drones exist only to mate. The "queen substance" [also termed the queen retinue pheromone (QRP)] is an eight-component pheromone that maintains the queen's dominance in the colony. The main component, 9-ODA, acts as a releaser pheromone by attracting workers to the queen and as a primer pheromone by physiologically inhibiting worker ovary development; it also acts as a sex pheromone, attracting drones during mating flights. However, the extent to which social and sexual chemical messages are shared remains unresolved. By using a custom chemosensory-specific microarray and qPCR, we identified four candidate sex pheromone Ors (AmOr10, -11, -18, and -170) from the honey bee genome based on their biased expression in drone antennae. We assayed the pheromone responsiveness of these receptors by using Xenopus oocytes and electrophysiology. AmOr11 responded specifically to 9-ODA (EC₅₀ = 280 ± 31 nM) and not to any of the other seven QRP components, other social pheromones, or floral odors. We did not observe any responses of the other three Ors to any of the eight QRP pheromone components, suggesting 9-ODA is the only QRP component that also acts as a long-distance sex pheromone. |
| doi_str_mv | 10.1073/pnas.0705459104 |
| format | Article |
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Honey bees live in large eusocial colonies in which a single queen is responsible for reproduction, several thousand sterile female worker bees complete a myriad of tasks to maintain the colony, and several hundred male drones exist only to mate. The "queen substance" [also termed the queen retinue pheromone (QRP)] is an eight-component pheromone that maintains the queen's dominance in the colony. The main component, 9-ODA, acts as a releaser pheromone by attracting workers to the queen and as a primer pheromone by physiologically inhibiting worker ovary development; it also acts as a sex pheromone, attracting drones during mating flights. However, the extent to which social and sexual chemical messages are shared remains unresolved. By using a custom chemosensory-specific microarray and qPCR, we identified four candidate sex pheromone Ors (AmOr10, -11, -18, and -170) from the honey bee genome based on their biased expression in drone antennae. We assayed the pheromone responsiveness of these receptors by using Xenopus oocytes and electrophysiology. AmOr11 responded specifically to 9-ODA (EC₅₀ = 280 ± 31 nM) and not to any of the other seven QRP components, other social pheromones, or floral odors. We did not observe any responses of the other three Ors to any of the eight QRP pheromone components, suggesting 9-ODA is the only QRP component that also acts as a long-distance sex pheromone.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.0705459104</identifier><identifier>PMID: 17761794</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>9-oxo-2-decenoic acid ; Animal behavior ; Animals ; antennae ; Apis mellifera ; Bees ; Bees - anatomy & histology ; Bees - metabolism ; Biological Sciences ; Drone honey bees ; Drone insects ; electroantennography ; Electrophysiology ; Fatty Acids, Monounsaturated - metabolism ; Female ; gene expression ; Genomics ; Honey bees ; Hormones ; Insect antennae ; Insect reproduction ; messenger RNA ; Odors ; olfactory receptors ; Oligopeptides - metabolism ; Oocytes ; Patch-Clamp Techniques ; Pheromones ; Phylogeny ; Queen honey bees ; queen pheromones ; queen retinue pheromone ; queen substance ; Receptors ; Receptors, Odorant - genetics ; Receptors, Odorant - metabolism ; Sex attractants ; sex pheromones ; smell ; Xenopus ; Xenopus laevis</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2007-09, Vol.104 (36), p.14383-14388</ispartof><rights>Copyright 2007 The National Academy of Sciences of the United States of America</rights><rights>Copyright National Academy of Sciences Sep 4, 2007</rights><rights>2007 by The National Academy of Sciences of the USA 2007</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c620t-4b822b6b92a3ec1238a8a739569b500322f6d8e980be73ad393649b557cbccf33</citedby><cites>FETCH-LOGICAL-c620t-4b822b6b92a3ec1238a8a739569b500322f6d8e980be73ad393649b557cbccf33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/104/36.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/25436680$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/25436680$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17761794$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wanner, Kevin W</creatorcontrib><creatorcontrib>Nichols, Andrew S</creatorcontrib><creatorcontrib>Walden, Kimberly K.O</creatorcontrib><creatorcontrib>Brockmann, Axel</creatorcontrib><creatorcontrib>Luetje, Charles W</creatorcontrib><creatorcontrib>Robertson, Hugh M</creatorcontrib><title>honey bee odorant receptor for the queen substance 9-oxo-2-decenoic acid</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>By using a functional genomics approach, we have identified a honey bee [Apis mellifera (Am)] odorant receptor (Or) for the queen substance 9-oxo-2-decenoic acid (9-ODA). Honey bees live in large eusocial colonies in which a single queen is responsible for reproduction, several thousand sterile female worker bees complete a myriad of tasks to maintain the colony, and several hundred male drones exist only to mate. The "queen substance" [also termed the queen retinue pheromone (QRP)] is an eight-component pheromone that maintains the queen's dominance in the colony. The main component, 9-ODA, acts as a releaser pheromone by attracting workers to the queen and as a primer pheromone by physiologically inhibiting worker ovary development; it also acts as a sex pheromone, attracting drones during mating flights. However, the extent to which social and sexual chemical messages are shared remains unresolved. By using a custom chemosensory-specific microarray and qPCR, we identified four candidate sex pheromone Ors (AmOr10, -11, -18, and -170) from the honey bee genome based on their biased expression in drone antennae. We assayed the pheromone responsiveness of these receptors by using Xenopus oocytes and electrophysiology. AmOr11 responded specifically to 9-ODA (EC₅₀ = 280 ± 31 nM) and not to any of the other seven QRP components, other social pheromones, or floral odors. We did not observe any responses of the other three Ors to any of the eight QRP pheromone components, suggesting 9-ODA is the only QRP component that also acts as a long-distance sex pheromone.</description><subject>9-oxo-2-decenoic acid</subject><subject>Animal behavior</subject><subject>Animals</subject><subject>antennae</subject><subject>Apis mellifera</subject><subject>Bees</subject><subject>Bees - anatomy & histology</subject><subject>Bees - metabolism</subject><subject>Biological Sciences</subject><subject>Drone honey bees</subject><subject>Drone insects</subject><subject>electroantennography</subject><subject>Electrophysiology</subject><subject>Fatty Acids, Monounsaturated - metabolism</subject><subject>Female</subject><subject>gene expression</subject><subject>Genomics</subject><subject>Honey bees</subject><subject>Hormones</subject><subject>Insect antennae</subject><subject>Insect reproduction</subject><subject>messenger RNA</subject><subject>Odors</subject><subject>olfactory receptors</subject><subject>Oligopeptides - metabolism</subject><subject>Oocytes</subject><subject>Patch-Clamp Techniques</subject><subject>Pheromones</subject><subject>Phylogeny</subject><subject>Queen honey bees</subject><subject>queen pheromones</subject><subject>queen retinue pheromone</subject><subject>queen substance</subject><subject>Receptors</subject><subject>Receptors, Odorant - genetics</subject><subject>Receptors, Odorant - metabolism</subject><subject>Sex attractants</subject><subject>sex pheromones</subject><subject>smell</subject><subject>Xenopus</subject><subject>Xenopus laevis</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkTtvFDEUhS0EIkugpgJGFEgUk1w_xo8GCUVAkCJRQGrL9niys5q1F9sTJf8ej3aVBZoUlovz3aN77kHoNYYzDIKe74LJZyCgY53CwJ6gFQaFW84UPEUrACJayQg7QS9y3gCA6iQ8RydYCI6FYit0uY7B3zfW-yb2MZlQmuSd35WYmqG-svbN79n70OTZ5mKC841q411sSdtXMMTRNcaN_Uv0bDBT9q8O_ym6_vrl18Vle_Xj2_eLz1et4wRKy6wkxHKriKHeYUKlkUZQ1XFlOwBKyMB76ZUE6wU1PVW0hrFdJ5x1bqD0FH3a--5mu_V93aAkM-ldGrcm3etoRv2vEsa1vom3GivOJCfV4MPBIMWaLBe9HbPz02SCj3PWXBIGArNHQQJEkkpX8P1_4CbOKdQrVAZTBoQvbud7yKWYc_LDw8oY9NKlXrrUxy7rxNu_kx75Q3kV-HgAlsmjHdOU6xpAUj3M01T8Xals8whbkTd7ZJNr-w8M6RjlXELV3-31wURtbtKY9fXPJSCABEYUp38ACcDExw</recordid><startdate>20070904</startdate><enddate>20070904</enddate><creator>Wanner, Kevin W</creator><creator>Nichols, Andrew S</creator><creator>Walden, Kimberly K.O</creator><creator>Brockmann, Axel</creator><creator>Luetje, Charles W</creator><creator>Robertson, Hugh M</creator><general>National Academy of Sciences</general><general>National Acad Sciences</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20070904</creationdate><title>honey bee odorant receptor for the queen substance 9-oxo-2-decenoic acid</title><author>Wanner, Kevin W ; 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Honey bees live in large eusocial colonies in which a single queen is responsible for reproduction, several thousand sterile female worker bees complete a myriad of tasks to maintain the colony, and several hundred male drones exist only to mate. The "queen substance" [also termed the queen retinue pheromone (QRP)] is an eight-component pheromone that maintains the queen's dominance in the colony. The main component, 9-ODA, acts as a releaser pheromone by attracting workers to the queen and as a primer pheromone by physiologically inhibiting worker ovary development; it also acts as a sex pheromone, attracting drones during mating flights. However, the extent to which social and sexual chemical messages are shared remains unresolved. By using a custom chemosensory-specific microarray and qPCR, we identified four candidate sex pheromone Ors (AmOr10, -11, -18, and -170) from the honey bee genome based on their biased expression in drone antennae. We assayed the pheromone responsiveness of these receptors by using Xenopus oocytes and electrophysiology. AmOr11 responded specifically to 9-ODA (EC₅₀ = 280 ± 31 nM) and not to any of the other seven QRP components, other social pheromones, or floral odors. We did not observe any responses of the other three Ors to any of the eight QRP pheromone components, suggesting 9-ODA is the only QRP component that also acts as a long-distance sex pheromone.</abstract><cop>United States</cop><pub>National Academy of Sciences</pub><pmid>17761794</pmid><doi>10.1073/pnas.0705459104</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 9-oxo-2-decenoic acid Animal behavior Animals antennae Apis mellifera Bees Bees - anatomy & histology Bees - metabolism Biological Sciences Drone honey bees Drone insects electroantennography Electrophysiology Fatty Acids, Monounsaturated - metabolism Female gene expression Genomics Honey bees Hormones Insect antennae Insect reproduction messenger RNA Odors olfactory receptors Oligopeptides - metabolism Oocytes Patch-Clamp Techniques Pheromones Phylogeny Queen honey bees queen pheromones queen retinue pheromone queen substance Receptors Receptors, Odorant - genetics Receptors, Odorant - metabolism Sex attractants sex pheromones smell Xenopus Xenopus laevis |
| title | honey bee odorant receptor for the queen substance 9-oxo-2-decenoic acid |
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