Four-Color DNA Sequencing by Synthesis on a Chip Using Photocleavable Fluorescent Nucleotides

We report four-color DNA sequencing by synthesis (SBS) on a chip, using four photocleavable fluorescent nucleotide analogues (dGTP-PC-Bodipy-FL-510, dUTP-PC-R6G, dATP-PC-ROX, and dCTP-PC-Bodipy-650) (PC, photocleavable; Bodipy, 4,4-difluoro-4-bora-3α, 4α-diaza-s-indacene; ROX, 6-carboxy-X-rhodamine;...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 2005-04, Vol.102 (17), p.5926-5931
Hauptverfasser:	Seo, Tae Seok, Bai, Xiaopeng, Kim, Dae Hyun, Meng, Qinglin, Shi, Shundi, Ruparel, Hameer, Li, Zengmin, Turro, Nicholas J., Ju, Jingyue
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Biological Sciences Chemistry Deoxyribonucleic acid DNA DNA - chemistry Fluorescence Fluorescent Dyes Functional groups Irradiation Lasers Ligation Molecular Sequence Data Nucleic Acid Conformation Nucleotides Oligonucleotide Array Sequence Analysis Photochemistry Physical Sciences Polymerase Chain Reaction Semiconductors Sequencing Templates, Genetic Washing
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