Genome-wide analysis of gene expression reveals function of the bZIP transcription factor HY5 in the UV-B response of Arabidopsis
The light environment is a key factor that governs a multitude of developmental processes during the entire life cycle of plants. An important and increasing part of the incident sunlight encompasses a segment of the UV-B region (280-320 nm) that is not entirely absorbed by the ozone layer in the st...
Gespeichert in:
| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2004-02, Vol.101 (5), p.1397-1402 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1402 |
|---|---|
| container_issue | 5 |
| container_start_page | 1397 |
| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 101 |
| creator | Ulm, R Baumann, A Oravecz, A Mate, Z Adam, E Oakeley, E.J Schafer, E Nagy, F |
| description | The light environment is a key factor that governs a multitude of developmental processes during the entire life cycle of plants. An important and increasing part of the incident sunlight encompasses a segment of the UV-B region (280-320 nm) that is not entirely absorbed by the ozone layer in the stratosphere of the earth. This portion of the solar radiation, which inevitably reaches the sessile plants, can act both as an environmental stress factor and an informational signal. To identify Arabidopsis genes involved in the UV response, we monitored the gene expression profile of UV-B-irradiated seedlings by using high-density oligonucleotide microarrays comprising almost the full Arabidopsis genome (>24,000 genes). A robust set of early low-level UV-B-responsive genes, 100 activated and 7 repressed, was identified. In all cases analyzed, UV-B induction was found to be independent of known photoreceptors. This group of genes is suggested to represent the molecular readout of the signaling cascade triggered by the elusive UV-B photoreceptor(s). Moreover, our analysis identified interactions between cellular responses to different UV-B ranges that led us to postulate the presence of partially distinct but interacting UV-B perception and signaling mechanisms. Finally, we demonstrate that the bZIP transcription factor HY5 is required for UV-B-mediated regulation of a subset of genes. |
| doi_str_mv | 10.1073/pnas.0308044100 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_pnas_</sourceid><recordid>TN_cdi_pnas_primary_101_5_1397_fulltext</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>3148846</jstor_id><sourcerecordid>3148846</sourcerecordid><originalsourceid>FETCH-LOGICAL-c546t-36b6b7a0499139dc25ea2b03bb3da053d76cf058a15d2462d20b688d48af7b2e3</originalsourceid><addsrcrecordid>eNqFkkFv1DAQhS0EosvCmQuCiAOc0o5jx3EOHEoFbaVKIMEiwcVyksnWq6yd2klpj_xznN1Vt3AA-RDJ73vPM5kh5DmFQwoFO-qtDofAQALnFOABmVEoaSp4CQ_JDCArUskzfkCehLACgDKX8JgcUF6wkjE5I79O0bo1pj9Ng4m2ursNJiSuTZZoMcGb3mMIxtnE4zXqLiTtaOthuojMcIlJ9eP8czJ4bUPtTb9RWl0Pzidn3_PE2A20-Ja-jwmhdzbg5Dz2ujKN6-NjT8mjNgbjs913ThYfP3w9OUsvPp2enxxfpHXOxZAyUYmq0MDLkrKyqbMcdVYBqyrWaMhZU4i6hVxqmjcZF1mTQSWkbLjUbVFlyObk3Ta3H6s1NjXaWHWnem_W2t8qp436U7HmUi3dtWKsAMGj_83O793ViGFQaxNq7Dpt0Y1BSaBcFLL8L0jLTICkNIKv_wJXbvRxBkFlQBlszpwcbaHauxA8tncVU1DTDqhpB9R-B6Lj5f1G9_xu6PeAybmPoypX8d8WEXj7T0C1Y9cNeDNE8sWWXIU48juUUS4lF1F-tZVb7ZReehPU4sumt1g5FbG931bS2Lg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>201303030</pqid></control><display><type>article</type><title>Genome-wide analysis of gene expression reveals function of the bZIP transcription factor HY5 in the UV-B response of Arabidopsis</title><source>Jstor Complete Legacy</source><source>MEDLINE</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>Ulm, R ; Baumann, A ; Oravecz, A ; Mate, Z ; Adam, E ; Oakeley, E.J ; Schafer, E ; Nagy, F</creator><creatorcontrib>Ulm, R ; Baumann, A ; Oravecz, A ; Mate, Z ; Adam, E ; Oakeley, E.J ; Schafer, E ; Nagy, F</creatorcontrib><description>The light environment is a key factor that governs a multitude of developmental processes during the entire life cycle of plants. An important and increasing part of the incident sunlight encompasses a segment of the UV-B region (280-320 nm) that is not entirely absorbed by the ozone layer in the stratosphere of the earth. This portion of the solar radiation, which inevitably reaches the sessile plants, can act both as an environmental stress factor and an informational signal. To identify Arabidopsis genes involved in the UV response, we monitored the gene expression profile of UV-B-irradiated seedlings by using high-density oligonucleotide microarrays comprising almost the full Arabidopsis genome (>24,000 genes). A robust set of early low-level UV-B-responsive genes, 100 activated and 7 repressed, was identified. In all cases analyzed, UV-B induction was found to be independent of known photoreceptors. This group of genes is suggested to represent the molecular readout of the signaling cascade triggered by the elusive UV-B photoreceptor(s). Moreover, our analysis identified interactions between cellular responses to different UV-B ranges that led us to postulate the presence of partially distinct but interacting UV-B perception and signaling mechanisms. Finally, we demonstrate that the bZIP transcription factor HY5 is required for UV-B-mediated regulation of a subset of genes.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.0308044100</identifier><identifier>PMID: 14739338</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>Adaptation ; AHY5 protein ; Arabidopsis - genetics ; Arabidopsis - radiation effects ; Arabidopsis Proteins ; Arabidopsis thaliana ; Basic-Leucine Zipper Transcription Factors ; Biological Sciences ; Botany ; DNA microarrays ; Gene expression ; Gene Expression Profiling ; Gene Expression Regulation, Plant - radiation effects ; Genes ; Genetics ; Genome, Plant ; Irradiation ; Nuclear Proteins - physiology ; Oligonucleotide Array Sequence Analysis ; Perception ; Photoreceptor Cells - physiology ; Photoreceptors ; Plants ; Promoter Regions, Genetic ; RNA ; Seedlings ; Transcription factors ; Transcription, Genetic ; Ultraviolet Rays ; Wavelengths</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2004-02, Vol.101 (5), p.1397-1402</ispartof><rights>Copyright 1993-2004 National Academy of Sciences of the United States of America</rights><rights>Copyright National Academy of Sciences Feb 3, 2004</rights><rights>Copyright © 2004, The National Academy of Sciences 2004</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c546t-36b6b7a0499139dc25ea2b03bb3da053d76cf058a15d2462d20b688d48af7b2e3</citedby><cites>FETCH-LOGICAL-c546t-36b6b7a0499139dc25ea2b03bb3da053d76cf058a15d2462d20b688d48af7b2e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/101/5.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3148846$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3148846$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14739338$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ulm, R</creatorcontrib><creatorcontrib>Baumann, A</creatorcontrib><creatorcontrib>Oravecz, A</creatorcontrib><creatorcontrib>Mate, Z</creatorcontrib><creatorcontrib>Adam, E</creatorcontrib><creatorcontrib>Oakeley, E.J</creatorcontrib><creatorcontrib>Schafer, E</creatorcontrib><creatorcontrib>Nagy, F</creatorcontrib><title>Genome-wide analysis of gene expression reveals function of the bZIP transcription factor HY5 in the UV-B response of Arabidopsis</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The light environment is a key factor that governs a multitude of developmental processes during the entire life cycle of plants. An important and increasing part of the incident sunlight encompasses a segment of the UV-B region (280-320 nm) that is not entirely absorbed by the ozone layer in the stratosphere of the earth. This portion of the solar radiation, which inevitably reaches the sessile plants, can act both as an environmental stress factor and an informational signal. To identify Arabidopsis genes involved in the UV response, we monitored the gene expression profile of UV-B-irradiated seedlings by using high-density oligonucleotide microarrays comprising almost the full Arabidopsis genome (>24,000 genes). A robust set of early low-level UV-B-responsive genes, 100 activated and 7 repressed, was identified. In all cases analyzed, UV-B induction was found to be independent of known photoreceptors. This group of genes is suggested to represent the molecular readout of the signaling cascade triggered by the elusive UV-B photoreceptor(s). Moreover, our analysis identified interactions between cellular responses to different UV-B ranges that led us to postulate the presence of partially distinct but interacting UV-B perception and signaling mechanisms. Finally, we demonstrate that the bZIP transcription factor HY5 is required for UV-B-mediated regulation of a subset of genes.</description><subject>Adaptation</subject><subject>AHY5 protein</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - radiation effects</subject><subject>Arabidopsis Proteins</subject><subject>Arabidopsis thaliana</subject><subject>Basic-Leucine Zipper Transcription Factors</subject><subject>Biological Sciences</subject><subject>Botany</subject><subject>DNA microarrays</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Plant - radiation effects</subject><subject>Genes</subject><subject>Genetics</subject><subject>Genome, Plant</subject><subject>Irradiation</subject><subject>Nuclear Proteins - physiology</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Perception</subject><subject>Photoreceptor Cells - physiology</subject><subject>Photoreceptors</subject><subject>Plants</subject><subject>Promoter Regions, Genetic</subject><subject>RNA</subject><subject>Seedlings</subject><subject>Transcription factors</subject><subject>Transcription, Genetic</subject><subject>Ultraviolet Rays</subject><subject>Wavelengths</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkkFv1DAQhS0EosvCmQuCiAOc0o5jx3EOHEoFbaVKIMEiwcVyksnWq6yd2klpj_xznN1Vt3AA-RDJ73vPM5kh5DmFQwoFO-qtDofAQALnFOABmVEoaSp4CQ_JDCArUskzfkCehLACgDKX8JgcUF6wkjE5I79O0bo1pj9Ng4m2ursNJiSuTZZoMcGb3mMIxtnE4zXqLiTtaOthuojMcIlJ9eP8czJ4bUPtTb9RWl0Pzidn3_PE2A20-Ja-jwmhdzbg5Dz2ujKN6-NjT8mjNgbjs913ThYfP3w9OUsvPp2enxxfpHXOxZAyUYmq0MDLkrKyqbMcdVYBqyrWaMhZU4i6hVxqmjcZF1mTQSWkbLjUbVFlyObk3Ta3H6s1NjXaWHWnem_W2t8qp436U7HmUi3dtWKsAMGj_83O793ViGFQaxNq7Dpt0Y1BSaBcFLL8L0jLTICkNIKv_wJXbvRxBkFlQBlszpwcbaHauxA8tncVU1DTDqhpB9R-B6Lj5f1G9_xu6PeAybmPoypX8d8WEXj7T0C1Y9cNeDNE8sWWXIU48juUUS4lF1F-tZVb7ZReehPU4sumt1g5FbG931bS2Lg</recordid><startdate>20040203</startdate><enddate>20040203</enddate><creator>Ulm, R</creator><creator>Baumann, A</creator><creator>Oravecz, A</creator><creator>Mate, Z</creator><creator>Adam, E</creator><creator>Oakeley, E.J</creator><creator>Schafer, E</creator><creator>Nagy, F</creator><general>National Academy of Sciences</general><general>National Acad Sciences</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20040203</creationdate><title>Genome-wide analysis of gene expression reveals function of the bZIP transcription factor HY5 in the UV-B response of Arabidopsis</title><author>Ulm, R ; Baumann, A ; Oravecz, A ; Mate, Z ; Adam, E ; Oakeley, E.J ; Schafer, E ; Nagy, F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c546t-36b6b7a0499139dc25ea2b03bb3da053d76cf058a15d2462d20b688d48af7b2e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Adaptation</topic><topic>AHY5 protein</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - radiation effects</topic><topic>Arabidopsis Proteins</topic><topic>Arabidopsis thaliana</topic><topic>Basic-Leucine Zipper Transcription Factors</topic><topic>Biological Sciences</topic><topic>Botany</topic><topic>DNA microarrays</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Plant - radiation effects</topic><topic>Genes</topic><topic>Genetics</topic><topic>Genome, Plant</topic><topic>Irradiation</topic><topic>Nuclear Proteins - physiology</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Perception</topic><topic>Photoreceptor Cells - physiology</topic><topic>Photoreceptors</topic><topic>Plants</topic><topic>Promoter Regions, Genetic</topic><topic>RNA</topic><topic>Seedlings</topic><topic>Transcription factors</topic><topic>Transcription, Genetic</topic><topic>Ultraviolet Rays</topic><topic>Wavelengths</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ulm, R</creatorcontrib><creatorcontrib>Baumann, A</creatorcontrib><creatorcontrib>Oravecz, A</creatorcontrib><creatorcontrib>Mate, Z</creatorcontrib><creatorcontrib>Adam, E</creatorcontrib><creatorcontrib>Oakeley, E.J</creatorcontrib><creatorcontrib>Schafer, E</creatorcontrib><creatorcontrib>Nagy, F</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ulm, R</au><au>Baumann, A</au><au>Oravecz, A</au><au>Mate, Z</au><au>Adam, E</au><au>Oakeley, E.J</au><au>Schafer, E</au><au>Nagy, F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genome-wide analysis of gene expression reveals function of the bZIP transcription factor HY5 in the UV-B response of Arabidopsis</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>2004-02-03</date><risdate>2004</risdate><volume>101</volume><issue>5</issue><spage>1397</spage><epage>1402</epage><pages>1397-1402</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>The light environment is a key factor that governs a multitude of developmental processes during the entire life cycle of plants. An important and increasing part of the incident sunlight encompasses a segment of the UV-B region (280-320 nm) that is not entirely absorbed by the ozone layer in the stratosphere of the earth. This portion of the solar radiation, which inevitably reaches the sessile plants, can act both as an environmental stress factor and an informational signal. To identify Arabidopsis genes involved in the UV response, we monitored the gene expression profile of UV-B-irradiated seedlings by using high-density oligonucleotide microarrays comprising almost the full Arabidopsis genome (>24,000 genes). A robust set of early low-level UV-B-responsive genes, 100 activated and 7 repressed, was identified. In all cases analyzed, UV-B induction was found to be independent of known photoreceptors. This group of genes is suggested to represent the molecular readout of the signaling cascade triggered by the elusive UV-B photoreceptor(s). Moreover, our analysis identified interactions between cellular responses to different UV-B ranges that led us to postulate the presence of partially distinct but interacting UV-B perception and signaling mechanisms. Finally, we demonstrate that the bZIP transcription factor HY5 is required for UV-B-mediated regulation of a subset of genes.</abstract><cop>United States</cop><pub>National Academy of Sciences</pub><pmid>14739338</pmid><doi>10.1073/pnas.0308044100</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 2004-02, Vol.101 (5), p.1397-1402 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_pnas_primary_101_5_1397_fulltext |
| source | Jstor Complete Legacy; MEDLINE; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Adaptation AHY5 protein Arabidopsis - genetics Arabidopsis - radiation effects Arabidopsis Proteins Arabidopsis thaliana Basic-Leucine Zipper Transcription Factors Biological Sciences Botany DNA microarrays Gene expression Gene Expression Profiling Gene Expression Regulation, Plant - radiation effects Genes Genetics Genome, Plant Irradiation Nuclear Proteins - physiology Oligonucleotide Array Sequence Analysis Perception Photoreceptor Cells - physiology Photoreceptors Plants Promoter Regions, Genetic RNA Seedlings Transcription factors Transcription, Genetic Ultraviolet Rays Wavelengths |
| title | Genome-wide analysis of gene expression reveals function of the bZIP transcription factor HY5 in the UV-B response of Arabidopsis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-06T21%3A03%3A16IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_pnas_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Genome-wide%20analysis%20of%20gene%20expression%20reveals%20function%20of%20the%20bZIP%20transcription%20factor%20HY5%20in%20the%20UV-B%20response%20of%20Arabidopsis&rft.jtitle=Proceedings%20of%20the%20National%20Academy%20of%20Sciences%20-%20PNAS&rft.au=Ulm,%20R&rft.date=2004-02-03&rft.volume=101&rft.issue=5&rft.spage=1397&rft.epage=1402&rft.pages=1397-1402&rft.issn=0027-8424&rft.eissn=1091-6490&rft_id=info:doi/10.1073/pnas.0308044100&rft_dat=%3Cjstor_pnas_%3E3148846%3C/jstor_pnas_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=201303030&rft_id=info:pmid/14739338&rft_jstor_id=3148846&rfr_iscdi=true |